Innate lymphoid cells, immune functional dynamics, epithelial parallels, and therapeutic frontiers in infections DOI
Wafa Nouari, Mourad Aribi

International Reviews of Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 28

Published: April 17, 2025

Innate lymphoid cells (ILCs) have emerged as pivotal players in the field of immunology, expanding our understanding innate immunity beyond conventional paradigms. This comprehensive review delves into multifaceted world ILCs, beginning with their serendipitous discovery and traversing ontogeny heterogeneity. We explore distinct subsets ILCs unraveling intriguing plasticity, which adds a layer complexity to functional repertoire. As we journey through activities address role immune responses against various infections, categorizing interactions helminthic parasites, bacterial pathogens, fungal viral invaders. Notably, this offers detailed examination context specific such Mycobacterium tuberculosis, Citrobacter rodentium, Clostridium difficile, Salmonella typhimurium, Helicobacter pylori, Listeria monocytogenes, Staphylococcus aureus, Pseudomonas aeruginosa, Influenza virus, Cytomegalovirus, Herpes simplex severe acute respiratory syndrome coronavirus 2. selection aimed for exploration infectious contexts, opting microorganisms based on extensive research findings rather than considerations virulence or emergence. Furthermore, raise questions about potential resemblances between epithelial cells, shedding light interconnectedness within mucosal microenvironment. The culminates critical assessment therapeutic prospects targeting during infection, emphasizing promise novel immunotherapeutic targets. Nevertheless, due recent evolving understanding, effectively manipulating is challenging. Ensuring specificity safety while evaluating long-term effects clinical settings will be crucial.

Language: Английский

Arsenic-Induced Modulation of Virulence and Drug Resistance in Pseudomonas aeruginosa DOI
Yingjie Song, Tao Li, Fang‐Jie Zhao

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 488, P. 137352 - 137352

Published: Jan. 24, 2025

Language: Английский

Citations

0
In Vitro and In Vivo Evaluation of the De Novo Designed Antimicrobial Peptide P6.2 Against a KPC-Producing P. aeruginosa Clinical Isolate DOI Creative Commons
Melina Martínez,

Merlina Corleto,

Melanie Weschenfeller

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 339 - 339

Published: Feb. 27, 2025

The antimicrobial peptide P6.2 was previously de novo designed as an alpha helix cationic amphipathic molecule. In previous work, we have shown that this displayed significant activity against both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria. However, while lacked biofilm-inhibiting properties the P. aeruginosa strain PA01, it anti-inflammatory effects in a murine acute lung infection model challenged with pathogen. its possible synergy meropenem were evaluated vitro vivo using Galleria mellonella carbapenem-resistant KPC-producing clinical isolate of aeruginosa. Firstly, cytotoxic effect on A549 RAW264.7 cell lines assayed, showing no cytotoxicity at 64 µg/mL below. Then, MIC (minimal inhibitory concentration) bactericidal carbapenemase-producing M13513 determined. showed between 32 µg/mL, rapid (less than 45 min). stability different temperatures bovine serum 37 °C also analyzed, good almost degradation after 15 min incubation 100 or 24 h serum, respectively. antibiofilm evaluated, although did not show biofilm activity, demonstrate disruptive together inside pre-formed biofilm. synergistic carbapenem then analyzed by killing kinetics, revealing interaction antibiotic strain. Finally, larvae model. Interestingly, G. mellonella, alone completely clear caused M13513. when combined meropenem, demonstrated effect, leading to increased survival rates infected larvae. results presented here highlight potential displays used combination carbapenems clinically relevant

Language: Английский

Citations

0
Revealing the impact of Pseudomonas aeruginosa quorum sensing molecule 2'-aminoacetophenone on human bronchial-airway epithelium and pulmonary endothelium using a human airway-on-a-chip DOI Open Access
Shifu Aggarwal, Arijit Chakraborty, Vijay Kumar Singh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Abstract Pseudomonas aeruginosa (PA) causes severe respiratory infections utilizing multiple virulence functions. Our previous findings on PA quorum sensing (QS)-regulated small molecule, 2’-aminoacetophenone (2-AA), secreted by the bacteria in infected tissues, revealed its effect immune and metabolic functions favouring a long-term presence of host. However, studies 2-AA’s specific effects bronchial-airway epithelium pulmonary endothelium remain elusive. To evaluate 2AA’s spatiotemporal changes human airway, considering endothelial cells as first point contact when route lung infection is hematogenic, we utilized microfluidic airway-on-chip lined polarized endothelium. Using this platform, performed RNA-sequencing to analyse responses 2-AA-treated primary microvascular (HPMEC) adjacent normal bronchial epithelial (NHBE) from healthy female donors potential cross-talk between these cells. Analyses unveiled signaling biosynthesis pathways be differentially regulated 2-AA cells, including HIF-1 pyrimidine signaling, glycosaminoglycan, glycosphingolipid biosynthesis, while were fatty acid metabolism, phosphatidylinositol estrogen receptor proinflammatory pathways. Significant overlap both cell types response was found genes implicated cellular In contrast, that related barrier permeability, cholesterol oxidative phosphorylation upon exposure studied. Murine in-vivo additional vitro culture confirmed accumulation Results also biomarkers associated with cystic fibrosis idiopathic modulated types, transmembrane regulator expression affected only The 2-AA-mediated within microphysiological dynamic environment mimicking airway enhance our understanding QS molecule. This study provides novel insights into their interactions, paving way for innovative, cell-specific therapeutic strategies combat infections.

Language: Английский

Citations

0
Unveiling the molecular epidemiology of Pseudomonas aeruginosa in lung infections among cystic fibrosis patients in the Brazilian Amazon DOI Creative Commons

Maria Isabel Montoril Gouveia,

Edilene do Socorro Nascimento Falcão Sarges, Herald Souza dos Reis

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 9, 2025

Pseudomonas aeruginosa is a major pathogen in cystic fibrosis (CF), where chronic and intermittent infections significantly affect patient outcomes. This study aimed to investigate the molecular epidemiology of P. CF patients from Brazilian Amazon, focusing on genotypic diversity, resistance profiles, virulence factors. A cross-sectional included 72 isolates 44 treated at regional reference center between 2018 2019. Antimicrobial susceptibility patterns were determined using VITEK-2 system Kirby-Bauer disk diffusion. Virulotypes defined by detection exoS, exoU, exoT, exoY, algU, algD genes. Genetic diversity was assessed multilocus sequence typing (MLST). Demographic data, clinical severity, spirometry results also collected. Among patients, 54.55% experienced infections, while 45.45% had infections. Chronic associated with older age, lower FEV1, reduced Shwachman-Kulczycki scores. Multidrug observed 15.3% isolates, particularly against ciprofloxacin piperacillin/tazobactam. The exoU gene present 55.56% an uncommon finding populations. High genetic evident, 37 types (STs), including 14 novel STs. High-risk clones (HRCs) constituted 25% ST274 being most prevalent (12.5%). Longitudinal analysis revealed transient colonization dominated stable clones. highlights dynamics Amazon. linked severe lung impairment , HRCs. These findings underscore need for robust surveillance mitigate burden

Language: Английский

Citations

0
Innate lymphoid cells, immune functional dynamics, epithelial parallels, and therapeutic frontiers in infections DOI
Wafa Nouari, Mourad Aribi

International Reviews of Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 28

Published: April 17, 2025

Innate lymphoid cells (ILCs) have emerged as pivotal players in the field of immunology, expanding our understanding innate immunity beyond conventional paradigms. This comprehensive review delves into multifaceted world ILCs, beginning with their serendipitous discovery and traversing ontogeny heterogeneity. We explore distinct subsets ILCs unraveling intriguing plasticity, which adds a layer complexity to functional repertoire. As we journey through activities address role immune responses against various infections, categorizing interactions helminthic parasites, bacterial pathogens, fungal viral invaders. Notably, this offers detailed examination context specific such Mycobacterium tuberculosis, Citrobacter rodentium, Clostridium difficile, Salmonella typhimurium, Helicobacter pylori, Listeria monocytogenes, Staphylococcus aureus, Pseudomonas aeruginosa, Influenza virus, Cytomegalovirus, Herpes simplex severe acute respiratory syndrome coronavirus 2. selection aimed for exploration infectious contexts, opting microorganisms based on extensive research findings rather than considerations virulence or emergence. Furthermore, raise questions about potential resemblances between epithelial cells, shedding light interconnectedness within mucosal microenvironment. The culminates critical assessment therapeutic prospects targeting during infection, emphasizing promise novel immunotherapeutic targets. Nevertheless, due recent evolving understanding, effectively manipulating is challenging. Ensuring specificity safety while evaluating long-term effects clinical settings will be crucial.

Language: Английский

Citations

0