Structural Characterization and Effect of Acetylated Polysaccharides From Flammulina Velutipes on High‐Fat Diet‐Induced Dyslipidemia DOI
Yu Xiao, Jinyi Li, Yiwen Zhang

et al.

Starch - Stärke, Journal Year: 2025, Volume and Issue: unknown

Published: May 16, 2025

ABSTRACT This study investigated the structural characteristics, in vitro antioxidant activity, and hypolipidemic effects of acetylated Flammulina velutipes polysaccharides (AFVP) mice with high‐fat diet (HFD)‐induced dyslipidemia. Structural characterization revealed that AFVP comprised β ‐glycosidic linked pyranose residues an average molecular weight 1.80 × 10⁴ Da, predominantly consisting mannose, glucose, galactose monomers. At 1200 mg/L concentration, demonstrated significant capacity a reducing power 2.25 ± 0.07, exhibiting hydroxyl radical scavenging activity 37.59% 1.26% DPPH elimination efficiency 87.12% 2.13%. Additionally, reduced liver levels IL‐6, LEP, TNF‐α, as well serum ALP, AST, ALT, LDL‐C, TC, TG. It enhanced activities SOD, GSH‐Px, CAT decreased MDA accumulation. Furthermore, treatment increased HDL‐C ADPN hyperlipidemic mice, while inhibiting fat These findings suggested exhibits HFD‐induced hyperlipidemia, providing potential therapeutic approach for hyperlipidemia laying foundation development natural‐based pharmaceuticals.

Language: Английский

Probing the Structural Elements of Polysaccharide Adjuvants for Enhancing Respiratory Mucosal Response: From Surmounting Multi-Obstacles to Eliciting Cascade Immunity DOI
Zhixiang Cui, Hezhi Wang, Qin Lu

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

The immunomodulatory effects and excellent tolerability of polysaccharides make them optimal candidates for pulmonary vaccine adjuvants. Yet, the structure-immunostimulatory activity relationship remains unrevealed. Here, we developed nanovaccines decorated with four distinct structures─hyaluronic acid (HA), pectin (PC), chondroitin sulfate (SC), heparan (SH)─all sharing similar particle sizes zeta potential. Polysaccharides containing groups (SC, SH) exhibited superior efficacy in overcoming natural inhalation barriers recruiting dendritic cells (DC). DC stimulation assays revealed that HA SH significantly upregulated expression costimulation signals, IL-6 secretion rising over 8.7-fold compared to pure OVA. Fluorescence resonance energy transfer demonstrated their detachment within lysosomal microenvironment, thereby enhancing antigen cross-presentation. However, vivo findings only showed CCR7 chemokine swiftly migrated lymph nodes. Molecular docking Western blot analyses further elucidated involvement TLR─MyD88─TRAF6─NF-κB/MAPK/IRF-7 signaling pathways. Notably, SH-modified induced a more robust cellular humoral immune response potential memory. This study confirms enhance activation combining acetyl offers promising adjuvant configuration augmenting mucosal, cellular, immunity.

Language: Английский

Citations

1
Screening of aqueous plant extracts for immunomodulatory effects on immune cells and cytokine production: in vitro and in vivo analyses DOI Creative Commons

Theerawat Dobutr,

Nisachon Jangpromma, Rina Patramanon

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(4), P. e42692 - e42692

Published: Feb. 1, 2025

This study investigates the immunomodulatory effects of various aqueous plant extracts on immune cells and cytokine production. In vitro, several extracts, including holy basil (Ocimum sanctum), patawali (Tinospora crispa), Indian borage (Plectranthus amboinicus L.), significantly increased CD3+ T-cell populations, while soap pod (Acacia concinna), garlic (Allium sativum neem (Azadirachta indica) also boosted CD45RA+ B-cells. vivo, had subtle spleen morphology Peyer's Patches, with milk bush (Euphorbia tirucalli L.) enhancing responses, stimulated Additionally, we observed that Neem suppressed B-cell populations. Furthermore, analysis showed reduced IL-2, pod, basil, TNF-alpha levels. Soap elevated IL-10 IL-17A, indicating both anti-inflammatory pro-inflammatory signaling, induced an increase in IL-4. conclusion, Thai medicinal plants show strong potential as immunostimulants immunosuppressants. They can enhance lymphocyte proliferation, particularly T-cells, modulate Their play a key role regulating Th1, Th2, Th17 Thus, these could serve natural agents alternative medicines for boosting or modulating function.

Language: Английский

Citations

0
Extraction methods, structural diversity and potential biological activities of Artemisia L. polysaccharides (APs): A review DOI
Adil Hussain

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142802 - 142802

Published: April 1, 2025

Language: Английский

Citations

0
Effects of pectin-type polysaccharides derived from Houttuynia cordata on the intestinal immune system in native C3H/HeN mice DOI

So Min Kim,

Hyun Young Shin,

Se Hyeon Sim

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142995 - 142995

Published: April 1, 2025

Language: Английский

Citations

0
Protective effects of jiawei yupingfeng polysaccharides on cyclophosphamide-induced intestinal injury in mice DOI

Runmin Wu,

Nianshou Zhao,

LI Hong-ya

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 310, P. 143405 - 143405

Published: April 22, 2025

Language: Английский

Citations

0
Immunomodulatory properties of an arabinan polysaccharide extracted from Sida cordifolia: isolation, characterization and in vitro mechanism of action DOI

Fang Yuan Cheng,

Xiaobei Pan, Anna Notaro

et al.

Carbohydrate Polymers, Journal Year: 2025, Volume and Issue: unknown, P. 123724 - 123724

Published: May 1, 2025

Language: Английский

Citations

0
Structural Characterization and Effect of Acetylated Polysaccharides From Flammulina Velutipes on High‐Fat Diet‐Induced Dyslipidemia DOI
Yu Xiao, Jinyi Li, Yiwen Zhang

et al.

Starch - Stärke, Journal Year: 2025, Volume and Issue: unknown

Published: May 16, 2025

ABSTRACT This study investigated the structural characteristics, in vitro antioxidant activity, and hypolipidemic effects of acetylated Flammulina velutipes polysaccharides (AFVP) mice with high‐fat diet (HFD)‐induced dyslipidemia. Structural characterization revealed that AFVP comprised β ‐glycosidic linked pyranose residues an average molecular weight 1.80 × 10⁴ Da, predominantly consisting mannose, glucose, galactose monomers. At 1200 mg/L concentration, demonstrated significant capacity a reducing power 2.25 ± 0.07, exhibiting hydroxyl radical scavenging activity 37.59% 1.26% DPPH elimination efficiency 87.12% 2.13%. Additionally, reduced liver levels IL‐6, LEP, TNF‐α, as well serum ALP, AST, ALT, LDL‐C, TC, TG. It enhanced activities SOD, GSH‐Px, CAT decreased MDA accumulation. Furthermore, treatment increased HDL‐C ADPN hyperlipidemic mice, while inhibiting fat These findings suggested exhibits HFD‐induced hyperlipidemia, providing potential therapeutic approach for hyperlipidemia laying foundation development natural‐based pharmaceuticals.

Language: Английский

Citations

0