Photoprotective Effects of Oral Pterostilbene in UVB-Induced Skin Photoaging BALB/c Mouse Model DOI Open Access
Raveena Vaidheswary Muralitharan,

Reda Omar,

Jing Ren Poh

et al.

Jundishapur Journal of Natural Pharmaceutical Products, Journal Year: 2025, Volume and Issue: 20(2)

Published: April 9, 2025

Background: Photoaging results from prolonged and repeated exposure to solar ultraviolet-B (UVB) radiation. While sunscreen is effective in preventing ultraviolet (UV)-induced skin damage, oral antioxidants may offer additional protective benefits. Pterostilbene (PS), a compound found blueberries Pterocarpus marsupium heartwood, recognized for its anti-aging anti-photocarcinogenic properties. However, photoprotective effects on photoaging have not been extensively studied. Objectives: This study investigates the of PS UVB-induced BALB/c mouse model. Methods: Sixteen 7-week-old female mice were randomly assigned groups using an online random number generator. The received daily doses corn oil or at 30 60 mg/kg oil. After 2-week period without UVB exposure, (-) group remained unexposed, while (+), (+) PS30, PS60 subjected increasing three times week eight weeks, resulting cumulative 3.7 J/cm2. Weekly pinch tests conducted throughout 8-week period. Photographs taken before culling, samples collected Hematoxylin & Eosin (H&E) staining, Masson's trichrome reduced glutathione (GSH) assay, melanin content assay. Results: PS30 showed erythema wrinkles, exhibited most significant improvement photoaged skin, with eliminated erythema, smoother skin. (4.05 ± 0.11 seconds) (3.65 0.06 significantly test compared (4.96 0.12 seconds), indicating improved elasticity. Additionally, (61.87 3.58 µm) (43.64 2.08 epidermal thickness (67.39 1.69 µm). Collagen increased (1.41 0.04) (1.27 0.02) (1.11 0.01). GSH levels (µmoL/mg) higher (0.56 0.01) (0.48 0.01), no difference between doses. Melanin (0.34 (0.35 (0.387 0.04). Conclusions: Oral demonstrated photoaging, suggesting potential as anti-photoaging agent cosmeceutical nutraceutical industries.

Language: Английский

Photoprotective Effects of Oral Pterostilbene in UVB-Induced Skin Photoaging BALB/c Mouse Model DOI Open Access
Raveena Vaidheswary Muralitharan,

Reda Omar,

Jing Ren Poh

et al.

Jundishapur Journal of Natural Pharmaceutical Products, Journal Year: 2025, Volume and Issue: 20(2)

Published: April 9, 2025

Background: Photoaging results from prolonged and repeated exposure to solar ultraviolet-B (UVB) radiation. While sunscreen is effective in preventing ultraviolet (UV)-induced skin damage, oral antioxidants may offer additional protective benefits. Pterostilbene (PS), a compound found blueberries Pterocarpus marsupium heartwood, recognized for its anti-aging anti-photocarcinogenic properties. However, photoprotective effects on photoaging have not been extensively studied. Objectives: This study investigates the of PS UVB-induced BALB/c mouse model. Methods: Sixteen 7-week-old female mice were randomly assigned groups using an online random number generator. The received daily doses corn oil or at 30 60 mg/kg oil. After 2-week period without UVB exposure, (-) group remained unexposed, while (+), (+) PS30, PS60 subjected increasing three times week eight weeks, resulting cumulative 3.7 J/cm2. Weekly pinch tests conducted throughout 8-week period. Photographs taken before culling, samples collected Hematoxylin & Eosin (H&E) staining, Masson's trichrome reduced glutathione (GSH) assay, melanin content assay. Results: PS30 showed erythema wrinkles, exhibited most significant improvement photoaged skin, with eliminated erythema, smoother skin. (4.05 ± 0.11 seconds) (3.65 0.06 significantly test compared (4.96 0.12 seconds), indicating improved elasticity. Additionally, (61.87 3.58 µm) (43.64 2.08 epidermal thickness (67.39 1.69 µm). Collagen increased (1.41 0.04) (1.27 0.02) (1.11 0.01). GSH levels (µmoL/mg) higher (0.56 0.01) (0.48 0.01), no difference between doses. Melanin (0.34 (0.35 (0.387 0.04). Conclusions: Oral demonstrated photoaging, suggesting potential as anti-photoaging agent cosmeceutical nutraceutical industries.

Language: Английский

Citations

0