Neurobiological Alterations Induced by SARS-CoV-2: Insights from Variant-Specific Host Gene Expression Patterns in hACE2-Expressing Mice

Viruses, Journal Year: 2025, Volume and Issue: 17(3), P. 329 - 329

Published: Feb. 27, 2025

Since the onset of COVID-19 pandemic, various severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants have emerged. Although primary site SARS-CoV-2 infection is lungs, it can also affect brain and induce neurological symptoms. However, specific effects different on remain unclear. In this study, a whole-transcriptome analysis was conducted using tissues K18-hACE2 mice infected with ancestral B.1 (Wuhan) variant major concern, including B.1.1.7 (Alpha), B.1.351 (Beta), B.1.617.2 (Delta) B.1.529 (Omicron). After sequencing, differential gene expression, ontology (GO) genome pathway enrichment analyses were performed. An Immune Cell Abundance Identifier (ImmuCellAI) used to identify abundance cell populations. Additionally, RT-qPCR validate RNA-seq data. The viral load hierarchical clustering divided samples into two clusters notable differences in expression at day 6 post-infection for all compared control group. GO Kyoto Encyclopedia genes genomes revealed similar patterns variants. ImmuCellAI changes immune populations, decrease CD4+ T B proportions increase CD8+ dendritic proportions. A co-expression network that some genes, such as STAT1, interleukin-6 (IL-6) tumor necrosis factor alpha (TNF-α), dysregulated IL-6, CXCL10 IRF7 further validated analysis. conclusion, study provides, first time, an extensive transcriptome mouse after

Language: Английский

Hederagenin ameliorates ferroptosis-induced damage by regulating PPARα/Nrf2/GPX4 signaling pathway in HT22 cells: An in vitro and in silico study

Bioorganic Chemistry, Journal Year: 2024, Volume and Issue: 155, P. 108119 - 108119

Published: Dec. 30, 2024

Language: Английский