Exosomes Derived From Human Gingival Mesenchymal Stem Cells Induce Metabolic Reprogramming of Inflammatory Macrophages DOI
Jialu Wang, Yingzhe Hu, Zhiguo Wang

et al.

Journal Of Clinical Periodontology, Journal Year: 2025, Volume and Issue: unknown

Published: May 19, 2025

ABSTRACT Aim To investigate the influence and mechanism of exosomes derived from human gingival mesenchymal stem cells (GMSC‐Exo) regulating macrophage polarisation through metabolic reprogramming. Materials Methods Human acute monocytic leukaemia (THP‐1)‐derived macrophages were treated with GMSC‐Exo or Porphyromonas gingivalis lipopolysaccharide ( P.g ‐LPS) in vitro. Metabolic inhibitors used to study role reprogramming GMSC‐Exo‐induced polarisation, while hypoxia‐inducible factor‐1 alpha (HIF‐1α) modulators employed explore HIF‐1α signalling pathway's impact on The periodontitis metabolism was assessed using a rat model vivo. Results In vitro experiments confirmed that promoted pro‐inflammatory M1 type (classically activated) anti‐inflammatory M2 (alternatively by promoting (glycolysis oxidative phosphorylation). this process, activation pathway inhibited. vivo revealed could regulate inflammatory microenvironment periodontal tissue pattern macrophages. Conclusion By inhibiting pathway, trigger macrophages, thereby transformation phenotype phenotype. This change enhances local environment, aiding repair regeneration.

Language: Английский

Combining Advanced Therapies with Alternative Treatments: A New Approach to Managing Antimicrobial Resistance? DOI Creative Commons
Greta Kaspute,

Arunas Zebrauskas,

Akvile Streckyte

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(5), P. 648 - 648

Published: May 15, 2025

Bacterial antimicrobial resistance (AMR) represents a critical public health threat, with increasing compromising the effectiveness of treatments worldwide. Resistance trends, such as fluctuating benzylpenicillin in Staphylococcus aureus, highlight growing urgency, projections indicating rise to various antibiotics, including complete gentamicin and tetracycline by 2027. Despite substantial efforts develop new antibiotics drug delivery systems, these approaches must undergo rigorous clinical evaluation ensure their safety efficacy. In parallel, alternative therapies, phytotherapy apitherapy, have garnered attention for potential combating infections. Natural substances like tea tree essential oils propolis, which exhibit properties, are being increasingly incorporated into novel systems. However, much research on materials is not new, several studies already exploring effectiveness. To address escalating AMR crisis, combining advanced therapies medicine could offer promising solution. Advanced therapy products target bacterial genomes enhance natural substances. This integrated approach remains underexplored pre-clinical trials, presenting future opportunities more effective strategies AMR. Given rapid spread resistant infections, there an urgent need innovative agents overcome emerging mechanisms improve diagnoses treatments.

Language: Английский

Citations

0
Exosomes Derived From Human Gingival Mesenchymal Stem Cells Induce Metabolic Reprogramming of Inflammatory Macrophages DOI
Jialu Wang, Yingzhe Hu, Zhiguo Wang

et al.

Journal Of Clinical Periodontology, Journal Year: 2025, Volume and Issue: unknown

Published: May 19, 2025

ABSTRACT Aim To investigate the influence and mechanism of exosomes derived from human gingival mesenchymal stem cells (GMSC‐Exo) regulating macrophage polarisation through metabolic reprogramming. Materials Methods Human acute monocytic leukaemia (THP‐1)‐derived macrophages were treated with GMSC‐Exo or Porphyromonas gingivalis lipopolysaccharide ( P.g ‐LPS) in vitro. Metabolic inhibitors used to study role reprogramming GMSC‐Exo‐induced polarisation, while hypoxia‐inducible factor‐1 alpha (HIF‐1α) modulators employed explore HIF‐1α signalling pathway's impact on The periodontitis metabolism was assessed using a rat model vivo. Results In vitro experiments confirmed that promoted pro‐inflammatory M1 type (classically activated) anti‐inflammatory M2 (alternatively by promoting (glycolysis oxidative phosphorylation). this process, activation pathway inhibited. vivo revealed could regulate inflammatory microenvironment periodontal tissue pattern macrophages. Conclusion By inhibiting pathway, trigger macrophages, thereby transformation phenotype phenotype. This change enhances local environment, aiding repair regeneration.

Language: Английский

Citations

0