LncRNA CYP1B1-AS1 as a clinical biomarker exacerbates sepsis inflammatory response via targeting miR- 18a- 5p DOI Creative Commons

Lixia Xu,

Jingpo Li,

Li Li

et al.

BMC Immunology, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 16, 2025

Sepsis, characterized by high morbidity and mortality, necessitates the identification of novel diagnostic prognostic biomarkers to enhance patient outcomes. Prior research has highlighted potential clinical utility long non-coding RNAs (lncRNAs) in sepsis. This study aimed investigate significance underlying mechanisms serum lncRNA Cytochrome P450 family 1 subfamily B member antisense RNA (CYP1B1-AS1) expression Differentially expressed lncRNAs sepsis patients were explored via GEO database. Sepsis Control subjects included. An vitro cellular model was established with LPS-stimulated THP- cells. RT-qPCR assessed CYP1B1-AS1 miR- 18a- 5p expression. ROC analysis evaluated predictive value. Kaplan-Meier curves Cox regression analyzed value CYP1B1-AS1. Flow cytometry ELISA cell apoptosis inflammatory factors levels. Dual luciferase reporter, RIP, pull down validate target binding relationship. The GSE217700 database shows that upregulated Serum levels higher than controls. positively correlated SOFA APACHE II scores distinguished from 28-day mortality rate for 29.31%. High predicts a worse prognosis is risk factor. targets 5p. Silencing reduced LPS-inducted factor promotion, which inhibitor reversed. serves as biomarker diagnosis poor prognosis, potentially promoting inflammation targeting

Language: Английский

LncRNA CYP1B1-AS1 as a clinical biomarker exacerbates sepsis inflammatory response via targeting miR- 18a- 5p DOI Creative Commons

Lixia Xu,

Jingpo Li,

Li Li

et al.

BMC Immunology, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 16, 2025

Sepsis, characterized by high morbidity and mortality, necessitates the identification of novel diagnostic prognostic biomarkers to enhance patient outcomes. Prior research has highlighted potential clinical utility long non-coding RNAs (lncRNAs) in sepsis. This study aimed investigate significance underlying mechanisms serum lncRNA Cytochrome P450 family 1 subfamily B member antisense RNA (CYP1B1-AS1) expression Differentially expressed lncRNAs sepsis patients were explored via GEO database. Sepsis Control subjects included. An vitro cellular model was established with LPS-stimulated THP- cells. RT-qPCR assessed CYP1B1-AS1 miR- 18a- 5p expression. ROC analysis evaluated predictive value. Kaplan-Meier curves Cox regression analyzed value CYP1B1-AS1. Flow cytometry ELISA cell apoptosis inflammatory factors levels. Dual luciferase reporter, RIP, pull down validate target binding relationship. The GSE217700 database shows that upregulated Serum levels higher than controls. positively correlated SOFA APACHE II scores distinguished from 28-day mortality rate for 29.31%. High predicts a worse prognosis is risk factor. targets 5p. Silencing reduced LPS-inducted factor promotion, which inhibitor reversed. serves as biomarker diagnosis poor prognosis, potentially promoting inflammation targeting

Language: Английский

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