Human regulatory γδT lymphocytes as novel autoimmunity-protective cells: Lessons from alopecia areata DOI Creative Commons
Amos Gilhar, Aviad Keren,

Nyra Goldstein

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Abstract Regulatory T cells control autoimmune diseases (AID). Yet, much less is known about the functions of evolutionarily older Foxp3 + human regulatory γδT (γδTregs). Here, we have explored these in one most common AID, hair loss disorder, alopecia areata (AA). Lesional AA skin showed significantly more γδTreg than non-lesional or healthy skin. Next, investigated how γδTregs impact on experimentally induced scalp xenotransplants SCID/beige mice. PBMC-derived autologous were pre-activated with IL-2, IL-15, and zoledronate in vitro injected intradermally into xenografts before after induction by CD8 vivo. not only prevented development lesions, but also promoted regrowth established lesions xenotransplants, accompanied a reduced perifollicular lymphocytic infiltrate restoration follicle (HF) immune privilege (IP) . We then co-cultured organ-cultured, stressed (MICA-overexpressing) HFs presence/absence pathogenic CD8+/NKG2D that induce HF IP collapse secreting interferon-g, all under conditions. Under ex vivo conditions, mitigated cells, primarily through IL-10 TGF-β1 secretion, enhanced keratinocyte proliferation their apoptosis while preventing premature catagen (= hallmarks). These findings model AID introduce as important lymphocytes invite novel cell-based therapies cell-dependent AIDs characterized such AA.

Language: Английский

Insulin resistance and cancer: molecular links and clinical perspectives DOI
Alfredo Caturano, Enes Erul,

Roberto Nilo

et al.

Molecular and Cellular Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 15, 2025

Language: Английский

Citations

0
Human regulatory γδT lymphocytes as novel autoimmunity-protective cells: Lessons from alopecia areata DOI Creative Commons
Amos Gilhar, Aviad Keren,

Nyra Goldstein

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Abstract Regulatory T cells control autoimmune diseases (AID). Yet, much less is known about the functions of evolutionarily older Foxp3 + human regulatory γδT (γδTregs). Here, we have explored these in one most common AID, hair loss disorder, alopecia areata (AA). Lesional AA skin showed significantly more γδTreg than non-lesional or healthy skin. Next, investigated how γδTregs impact on experimentally induced scalp xenotransplants SCID/beige mice. PBMC-derived autologous were pre-activated with IL-2, IL-15, and zoledronate in vitro injected intradermally into xenografts before after induction by CD8 vivo. not only prevented development lesions, but also promoted regrowth established lesions xenotransplants, accompanied a reduced perifollicular lymphocytic infiltrate restoration follicle (HF) immune privilege (IP) . We then co-cultured organ-cultured, stressed (MICA-overexpressing) HFs presence/absence pathogenic CD8+/NKG2D that induce HF IP collapse secreting interferon-g, all under conditions. Under ex vivo conditions, mitigated cells, primarily through IL-10 TGF-β1 secretion, enhanced keratinocyte proliferation their apoptosis while preventing premature catagen (= hallmarks). These findings model AID introduce as important lymphocytes invite novel cell-based therapies cell-dependent AIDs characterized such AA.

Language: Английский

Citations

0