Elucidating stearoyl metabolism and NCOA4-mediated ferroptosis in gastric cancer liver metastasis through multi-omics single-cell integrative mendelian analysis: advancing personalized immunotherapy strategies

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 15, 2025

The metabolism of stearoyl-GPE plays a key role in the liver metastasis gastric cancer. This investigation delves into mechanisms underlying intricate tumor microenvironment (TME) heterogeneity triggered by stearoyl cancer with (LMGC), offering novel perspectives for LMGC. Utilizing Mendelian randomization, we determined that significantly contributes to progression (GC). Following this, bulk transcriptome analyses and single-cell multiomics techniques investigate roles metabolism-related genes, particularly NCOA4, regulating LMGC TME. Our analysis highlights crucial modulating complex LMGC, impacting monocyte cells. Through sequencing spatial transcriptomics, have identified metabolic genes specific within cell population, including NCOA4. Regarding relationship between ferroptosis, metabolism, findings, it is plausible pathways intersect involved ferroptosis. Ferroptosis, characterized iron-dependent lipid peroxidation, represents regulated form death. activity Stearoyl-CoA desaturase (SCD), critical enzyme has been associated modulation composition susceptibility Furthermore, integral cellular processes related oxidative stress both which are significant factors context study enhances understanding ferroptosis promoting its regulation heterogeneity. In addition, this deeper dynamics provides basis development better interventions combat metastasis.

Language: Английский

Integrating multi-omics techniques and in vitro experiments reveals that GLRX3 regulates the immune microenvironment and promotes hepatocellular carcinoma cell proliferation and invasion through iron metabolism pathways

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 25, 2024

Hepatocellular carcinoma (HCC) is a common malignancy worldwide, and its development closely related to abnormalities in iron metabolism. This study aims systematically analyze changes metabolism the tumor microenvironment of HCC using single-cell sequencing technology, investigate potential mechanisms by which regulation affects survival liver cancer patients.

Language: Английский