Look Beyond Plasma Membrane Biophysics: Revealing Considerable Variability of the Dipole Potential Between Plasma and Organelle Membranes of Living Cells DOI Open Access
Máté Szabó, Bence Cs. Szabo,

Kitti Kurtan

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 889 - 889

Published: Jan. 22, 2025

Due to the lack of measurement techniques suitable for examining compartments intact, living cells, membrane biophysics is almost exclusively investigated in plasma despite fact that its alterations intracellular organelles may also contribute disease pathogenesis. Here, we employ a novel, easy-to-use, confocal microscopy-based approach utilizing F66, an environment-sensitive fluorophore combination with fluorescent organelle markers and quantitative image analysis determine magnitude molecular order-related dipole potential various tumor neural cell lines. Our comparative demonstrates considerable variations be large enough modulate protein functions, inward decreasing gradient on route secretory/endocytic pathway (plasma >> lysosome > Golgi endoplasmic reticulum), whereas mitochondrial membranes are characterized by slightly larger than lysosomes. sensitive quantify biophysical properties selectively their and, therefore, identify affected therapeutic targets diseases associated lipid composition thus such as tumors, metabolic, neurodegenerative, or lysosomal storage disorders.

Language: Английский

Look Beyond Plasma Membrane Biophysics: Revealing Considerable Variability of the Dipole Potential Between Plasma and Organelle Membranes of Living Cells DOI Open Access
Máté Szabó, Bence Cs. Szabo,

Kitti Kurtan

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 889 - 889

Published: Jan. 22, 2025

Due to the lack of measurement techniques suitable for examining compartments intact, living cells, membrane biophysics is almost exclusively investigated in plasma despite fact that its alterations intracellular organelles may also contribute disease pathogenesis. Here, we employ a novel, easy-to-use, confocal microscopy-based approach utilizing F66, an environment-sensitive fluorophore combination with fluorescent organelle markers and quantitative image analysis determine magnitude molecular order-related dipole potential various tumor neural cell lines. Our comparative demonstrates considerable variations be large enough modulate protein functions, inward decreasing gradient on route secretory/endocytic pathway (plasma >> lysosome > Golgi endoplasmic reticulum), whereas mitochondrial membranes are characterized by slightly larger than lysosomes. sensitive quantify biophysical properties selectively their and, therefore, identify affected therapeutic targets diseases associated lipid composition thus such as tumors, metabolic, neurodegenerative, or lysosomal storage disorders.

Language: Английский

Citations

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