Lactobacillus johnsonii N6.2 Phospholipids Induce T Cell Anergy upon Cognate Dendritic Cell Interactions DOI Creative Commons
Alexandra Cuaycal, Monica Lamberti, Graciela L. Lorca

et al.

Metabolites, Journal Year: 2025, Volume and Issue: 15(5), P. 284 - 284

Published: April 22, 2025

Background/Objectives: Lactobacillus johnsonii N6.2 is a gut symbiont with probiotic properties. L. delayed the progression of type 1 diabetes (T1D) in diabetic-prone rats. The intake demonstrated immune cell modulation healthy volunteers, leading to improved wellness and fewer reported symptoms like headaches abdominal pain. These systemic immune-modulating benefits are attributed N6.2’s bioactive fractions, including extracellular vesicles (EVs) purified phospholipids (PLs). We have previously shown that PLs modulate dendritic (DC) function towards regulatory-like phenotype. Here, we further characterize regulatory effects on adaptive immunity, specifically upon DC T interactions. hypothesized PL-stimulated DCs suppress cell-mediated responses maintain tolerance intra- extra-intestinal sites. Methods: Bone marrow-derived cells (BMDCs) were generated from Sprague-Dawley rats stimulated PLs. Isogenic isolated PBMCs obtained via terminal exsanguination. In vitro cellular assays, co-culture experiments, gene expression analysis by qRT-PCR, flow cytometry assays conducted assess Results: BMDCs upregulated markers exhibited an immature-like phenotype reduced surface maturation but increased migratory molecules (ICAM-1). presented immunosuppressive functions cognate interactions presence TCR stimulation. Specifically, BMCDs suppressed Th1 effector induced anergy-related genes after co-culturing for 72 h. Conclusions: This study highlights capacity components DC:T immunosuppression. Our findings suggest N6.2-purified play role regulating offering potential managing immune-related diseases T1D.

Language: Английский

Lactobacillus johnsonii N6.2 Phospholipids Induce T Cell Anergy upon Cognate Dendritic Cell Interactions DOI Creative Commons
Alexandra Cuaycal, Monica Lamberti, Graciela L. Lorca

et al.

Metabolites, Journal Year: 2025, Volume and Issue: 15(5), P. 284 - 284

Published: April 22, 2025

Background/Objectives: Lactobacillus johnsonii N6.2 is a gut symbiont with probiotic properties. L. delayed the progression of type 1 diabetes (T1D) in diabetic-prone rats. The intake demonstrated immune cell modulation healthy volunteers, leading to improved wellness and fewer reported symptoms like headaches abdominal pain. These systemic immune-modulating benefits are attributed N6.2’s bioactive fractions, including extracellular vesicles (EVs) purified phospholipids (PLs). We have previously shown that PLs modulate dendritic (DC) function towards regulatory-like phenotype. Here, we further characterize regulatory effects on adaptive immunity, specifically upon DC T interactions. hypothesized PL-stimulated DCs suppress cell-mediated responses maintain tolerance intra- extra-intestinal sites. Methods: Bone marrow-derived cells (BMDCs) were generated from Sprague-Dawley rats stimulated PLs. Isogenic isolated PBMCs obtained via terminal exsanguination. In vitro cellular assays, co-culture experiments, gene expression analysis by qRT-PCR, flow cytometry assays conducted assess Results: BMDCs upregulated markers exhibited an immature-like phenotype reduced surface maturation but increased migratory molecules (ICAM-1). presented immunosuppressive functions cognate interactions presence TCR stimulation. Specifically, BMCDs suppressed Th1 effector induced anergy-related genes after co-culturing for 72 h. Conclusions: This study highlights capacity components DC:T immunosuppression. Our findings suggest N6.2-purified play role regulating offering potential managing immune-related diseases T1D.

Language: Английский

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