Enhancing seafood traceability: tracking the origin of seabass and seabream from the tuscan coast area by the analysis of the gill bacterial communities

Animal Microbiome, Journal Year: 2024, Volume and Issue: 6(1)

Published: March 14, 2024

The seafood consumption and trade have increased over the years, along its expected expansion pose major challenges to industry government institutions. In particular, global in fish products consequent are linked reliable authentication, necessary guarantee lawful healthy consumption. Alterations or errors this process can lead commercial fraud and/or health threats. Consequently, development of new investigative tools became crucial ensuring unwanted scenarios. Here we used NGS techniques through targeted metagenomics approach on V3-V4 region 16S rRNA genes characterize gill bacterial communities wild-caught seabream (Sparus aurata) seabass (Dicentrarchus labrax) within different fisheries areas "Costa degli Etruschi'' area Tuscan coast. Our challenge involved possibility discriminating between microbiota both species collected from three fishing sites very close each other (all 100 km) important a tourist point view.

Language: Английский

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Molecular interactions in an holobiont-pathogen model: Integromics in gilthead seabream infected with Sparicotyle chrysophrii

Aquaculture, Journal Year: 2023, Volume and Issue: 581, P. 740365 - 740365

Published: Nov. 20, 2023

The gill ectoparasite Sparicotyle chrysophrii is the major parasitic pathogen in gilthead seabream (Sparus aurata) aquaculture with limited management and treatment strategies. In this work, we assessed modulation of microbiota during sparicotylosis by sequencing complete 16S rRNA gene, host's molecular pathways occurring at local systemic levels performing RNA gill, spleen liver. results obtained other available data from same animals were integrated to unveil routes interactions host-microbiota-parasite system. Sparicotylosis significantly modulated decreasing diversity fish high infection intensity (H). LEfSe analysis revealed nine microbial biomarkers, highlighting 2013Ark19i (Candidatus Ichthyocystis sparus), already present control fish, but dramatically increasing H fish. This bacterium, proposed as causing agent epitheliocystis, correlated bacterial cysts observed histopathology, revealing a secondary these animals. study, 759, 337, 603 differentially expressed transcripts (DET) found gills, liver, respectively, 16 commonly regulated transcripts, including genes related parasite pathogenesis, such haemoglobin subunits or hypoxia-inducible factors. Relevant infected immune system; response stress, starvation hypoxia; apoptosis; haemostasis. Several DET enriched shared previous plasma proteomics particularly integrative separated specifically affected primary (parasite) (bacterium) infections. These show how changes microbiota, facilitating growth pathogenic bacteria development infections aggravating disease outcome. They also evidence host involved pathogenesis. Key identified using approach contribute understanding can constitute targets for further research pointing solutions infection.

Language: Английский

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Deciphering spread of quinolone resistance in mariculture ponds: Cross-species and cross-environment transmission of resistome

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 487, P. 137198 - 137198

Published: Jan. 17, 2025

Language: Английский

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Relationship between otolith size and fish total length of Saurida lessepsianus Russell, Golani and Tikochinski, 2015 collected from the coast of Muğla province, Aegean Sea, Türkiye

Acta Zoologica, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Abstract The relationship between fish total length and otolith length, width weight was studied in the synodontid species Saurida lessepsianus collected from coast of Muğla province, Aegean Sea, Türkiye. is regarded as a marketable objective study at hand to evaluate statistical association weight. This evidence will be significant future investigations on biology this species, which offer key data about feeding, age growth species. Linear non‐linear regression models were utilized. results obtained examination first address traits for S. Turkish marine waters.

Language: Английский

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Network-based integration of omics, physiological and environmental data in real-world Elbe estuarine Zander

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 942, P. 173656 - 173656

Published: June 1, 2024

Coastal and estuarine environments are under endogenic exogenic pressures jeopardizing survival diversity of inhabiting biota. Information possible synergistic effects multiple (a)biotic stressors holobiont interaction largely missing in estuaries like the Elbe but importance to estimate unforeseen on animals' physiology. Here, we seek leverage host-transcriptional RNA-seq gill mucus microbial 16S rRNA metabarcoding data coupled with physiological abiotic measurements a network analysis approach decipher impact health juvenile Sander lucioperca along one largest European estuaries. We find mesohaline areas characterized by tissue specific transcriptional responses matching osmosensing remodeling. Liver transcriptomes instead emphasized that zander from highly turbid were undergoing starvation which was supported compromised body condition. Potential pathogenic bacteria, including Shewanella, Acinetobacter, Aeromonas Chryseobacterium, dominated microbiome freshwater transition oxygen minimum zone. Their occurrence coincided strong adaptive innate immune response host enhanced energy demand liver supporting their potential pathogenicity. Taken together, show fish species its factors whose is expected increase consequences climate change. further present method for close-meshed detection main bacterial disease productive ecosystem.

Language: Английский

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Reveal your microbes, and i’ll reveal your origins: geographical traceability via Scomber colias intestinal tract metagenomics

Animal Microbiome, Journal Year: 2025, Volume and Issue: 7(1)

Published: May 6, 2025

The commercial demand for small pelagic fish, such as Atlantic chub mackerel (Scomber colias), renders them susceptible to provenance fraud. Scomber colias specimens intestinal tract bacteriome from five distinct fishing areas along the Portuguese coastline were analyzed by 4th-generation sequencing. Bacteria diversity indices and differential abundance revealed dissimilarities in operational taxonomic unit (OTU) among sites. Random forest-based model yielded an 85% accuracy rate attributing sample based on OTU relative abundance. Further refinement of microbial features using Indicator Species Analysis, Linear Discriminant Analysis Effect Size (LEfSe) Gini scores enabled identification 3-5 bacterial location biomarkers per site. sequences linked pathogenic bacteria, particularly Center-North Center-South areas. While this doesn't imply active pathogens, it highlights potential public health concerns complements efforts improve seafood microbiological quality traceability.

Language: Английский

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Low salinity activates a virulence program in the generalist marine pathogen Photobacterium damselae subsp. damselae

mSystems, Journal Year: 2023, Volume and Issue: 8(3)

Published: June 8, 2023

Facultative marine bacterial pathogens sense environmental signals so that the expression of virulence factors is upregulated on entry into hosts and downregulated during free-living lifestyle in environment. In this study, we utilized transcriptome sequencing to compare transcriptional profiles Photobacterium damselae subsp. damselae, a generalist pathogen causes disease diverse animals fatal infections humans at NaCl concentrations mimic or host internal milieu, respectively. We here show concentration constitutes major regulatory signal shapes uncover 1,808 differentially expressed genes (888 920 response low-salt conditions). Growth 3% NaCl, salinity mimics lifestyle, involved energy production, nitrogen metabolism, transport compatible solutes, utilization trehalose fructose, carbohydrate amino acid metabolism with strong upregulation arginine deiminase system (ADS). addition, observed marked increase resistance antibiotics NaCl. On contrary, low conditions (1% NaCl) those encountered triggered gene profile maximized production type 2 secretion (T2SS)-dependent cytotoxins damselysin, phobalysin P, putative PirAB-like toxin, observations were corroborated by analysis secretome. Low also iron-acquisition systems, efflux pumps, other functions related stress virulence. The results study greatly expand our knowledge salinity-responsive adaptations versatile pathogen. IMPORTANCE Pathogenic Vibrionaceae species experience continuous shifts their life cycles. However, impact changes regulation has been studied small number Vibrio species. analyzed (Pdd), facultative pathogen, salinity, demonstrate growth 1% comparison triggers program expression, T2SS-dependent decrease bacteria proposed constitute upregulates genetic invasion tissue damage, nutrient scavenging (notably iron), responses. This will surely inspire new research Pdd pathobiology, as well important family taxa whose regulons still await investigation.

Language: Английский

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Molecular Identification of Photobacterium damselae in Wild Marine Fish from the Eastern Mediterranean Sea

Fishes, Journal Year: 2023, Volume and Issue: 8(2), P. 60 - 60

Published: Jan. 18, 2023

Infectious diseases caused by marine bacterial pathogens inflict increasing economic losses to fisheries and aquaculture, while also posing a growing risk public health affected species conservation. In this study, four wild fish were collected at five fishing sites in Israel, divided into two regions—north (Acre, Haifa, Shefayim) center-south (Tel-Aviv Ashdod), screened for Photobacterium damselae. An initial screening was carried out using PCR analysis with specifically designed primers on DNA extracted from livers kidneys. P. damselae-positive samples had their 16S rRNA amplicons sequenced. Later, an attempt specify relevant sub-species performed, three-layered gene screen: Car, ureC toxR. Of 334 samples, 47 (14%) found be damselae-positive, of which 20 identified as damselae subsp. piscicida (Phdp), (Phdd) 25 could not subspecies. Our results strengthen the view that residing polluted environment are receptive pathogenic microorganisms. To assess how presence may affect population management conservation, research should followed studies aimed at: (i) quantifying levels pollutants pathogen emergence, (ii) creating standard pollution-level index basis setting criteria, above authorities take measures precaution.

Language: Английский

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Bacterial community trends associated with sea scallop, Placopecten magellanicus, larvae in a hatchery system

Aquaculture Reports, Journal Year: 2023, Volume and Issue: 32, P. 101693 - 101693

Published: Aug. 3, 2023

Atlantic sea scallops, Placopecten magellanicus, are the most economically important marine bivalves along northeastern coast of North America. Wild harvest landings generate hundreds millions dollars, and wild-caught adults juvenile spat increasingly being cultured in aquaculture facilities coastal farms. However, last two weeks larval maturation phase hatcheries often plagued by large mortality events. Research into other scallop- aquacultured-species point to bacterial infections or altered functionality microbial communities which associate with host. Despite intense filtering sterilization seawater, changing tank water every 48 h, harmful microbes can still persist biofilms is high. There no previous studies associated growing scallop hatchery tanks, nor wild scallops. We characterized veliger-stage larvae, using 16 S rRNA gene V3-V4 region sequenced on Illumina MiSeq platform. Hatchery larvae had lower richness (number bacteria taxa present) than biofilms, a similar community (which were both biofilms. Bacterial similarity between samples fluctuated over trial repeated patterns rise fall, showed some correlation lunar cycle that may be proxy for effects spring tides trends seawater phages propagated tanks. These results future work, will inform methods increase survival these facilities, example, implementing additional avoiding collection during tides, reduce concern promote more diverse would compete against pathogens.

Language: Английский

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The gills and skin microbiota of five pelagic fish species from the Atlantic Ocean

International Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: May 13, 2024

Abstract The gills and skin microbiota microbiome of wild fish remain far more under-investigated compared to that farmed species, despite these animal-microbe interactions hold the same ecophysiological roles in both cases. In this study, bacterial profiles their presumptive metabolisms were investigated five open-sea fishes: bullet tuna ( Auxis sp.), common dolphinfish Coryphaena hippurus ), Atlantic little tunny Euthynnus alletteratus bonito Sarda sarda ) white marlin Kajikia albida ). Gills tissues collected from two three individuals per specimens caught by recreational trolling during summer 2019, 16S rRNA gene diversity was analysed high-throughput sequencing. communities among species clearly different but not microbiota. dominant operational taxonomic units belonged Moraxellaceae, Pseudomonadaceae, Rhodobacteraceae, Staphylococcaceae Vibrionaceae families. Despite differences composition, between fishes here ≥ 94% similar dominated basic metabolism, most likely reflecting continuous exposure surrounding seawater.

Language: Английский

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