Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Aug. 8, 2023
Abstract
Sun-drying
is
a
traditional
process
for
preparing
dried
shrimp
in
coastal
area
of
South
China,
but
its
impacts
on
nutrition
and
the
formation
flavor-contributory
substances
remain
largely
unknown.
This
study
aimed
to
examine
effects
production
microbiota
metabolites
shrimp.
16S
rDNA
amplicon
sequencing
was
employed
identify
170
operational
taxonomic
units
(OTUs),
with
Vibrio
,
Photobacterium
Shewanella
emerging
as
primary
pathogenic
bacteria
samples.
Lactococcus
lactis
identified
principal
potential
probiotic
accrue
during
process,
found
contribute
significantly
development
desirable
flavors.
LC-MS-based
analyses
sample
metabolomes
revealed
notable
increase
compounds
associated
unsaturated
fatty
acid
biosynthesis,
arachidonic
metabolism,
amino
flavonoid
flavanol
biosynthesis
throughout
drying
process.
Subsequent
exploration
relationship
between
bacterial
flora
highlighted
predominant
coexistence
Bifidobacterium
Clostridium
contributing
heterocyclic
organic
acids
their
derivatives.
Conversely,
Arthrobacter
Staphylococcus
were
inhibit
each
other,
primarily
presence
compounds.
comprehensive
investigation
provides
valuable
insights
into
dynamic
changes
shrimps
spanning
different
periods,
which
we
expect
enhancing
techniques
safety
measures
processing.
Animal Microbiome,
Journal Year:
2024,
Volume and Issue:
6(1)
Published: March 14, 2024
The
seafood
consumption
and
trade
have
increased
over
the
years,
along
its
expected
expansion
pose
major
challenges
to
industry
government
institutions.
In
particular,
global
in
fish
products
consequent
are
linked
reliable
authentication,
necessary
guarantee
lawful
healthy
consumption.
Alterations
or
errors
this
process
can
lead
commercial
fraud
and/or
health
threats.
Consequently,
development
of
new
investigative
tools
became
crucial
ensuring
unwanted
scenarios.
Here
we
used
NGS
techniques
through
targeted
metagenomics
approach
on
V3-V4
region
16S
rRNA
genes
characterize
gill
bacterial
communities
wild-caught
seabream
(Sparus
aurata)
seabass
(Dicentrarchus
labrax)
within
different
fisheries
areas
"Costa
degli
Etruschi''
area
Tuscan
coast.
Our
challenge
involved
possibility
discriminating
between
microbiota
both
species
collected
from
three
fishing
sites
very
close
each
other
(all
100
km)
important
a
tourist
point
view.
Aquaculture,
Journal Year:
2023,
Volume and Issue:
581, P. 740365 - 740365
Published: Nov. 20, 2023
The
gill
ectoparasite
Sparicotyle
chrysophrii
is
the
major
parasitic
pathogen
in
gilthead
seabream
(Sparus
aurata)
aquaculture
with
limited
management
and
treatment
strategies.
In
this
work,
we
assessed
modulation
of
microbiota
during
sparicotylosis
by
sequencing
complete
16S
rRNA
gene,
host's
molecular
pathways
occurring
at
local
systemic
levels
performing
RNA
gill,
spleen
liver.
results
obtained
other
available
data
from
same
animals
were
integrated
to
unveil
routes
interactions
host-microbiota-parasite
system.
Sparicotylosis
significantly
modulated
decreasing
diversity
fish
high
infection
intensity
(H).
LEfSe
analysis
revealed
nine
microbial
biomarkers,
highlighting
2013Ark19i
(Candidatus
Ichthyocystis
sparus),
already
present
control
fish,
but
dramatically
increasing
H
fish.
This
bacterium,
proposed
as
causing
agent
epitheliocystis,
correlated
bacterial
cysts
observed
histopathology,
revealing
a
secondary
these
animals.
study,
759,
337,
603
differentially
expressed
transcripts
(DET)
found
gills,
liver,
respectively,
16
commonly
regulated
transcripts,
including
genes
related
parasite
pathogenesis,
such
haemoglobin
subunits
or
hypoxia-inducible
factors.
Relevant
infected
immune
system;
response
stress,
starvation
hypoxia;
apoptosis;
haemostasis.
Several
DET
enriched
shared
previous
plasma
proteomics
particularly
integrative
separated
specifically
affected
primary
(parasite)
(bacterium)
infections.
These
show
how
changes
microbiota,
facilitating
growth
pathogenic
bacteria
development
infections
aggravating
disease
outcome.
They
also
evidence
host
involved
pathogenesis.
Key
identified
using
approach
contribute
understanding
can
constitute
targets
for
further
research
pointing
solutions
infection.
Acta Zoologica,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 13, 2025
Abstract
The
relationship
between
fish
total
length
and
otolith
length,
width
weight
was
studied
in
the
synodontid
species
Saurida
lessepsianus
collected
from
coast
of
Muğla
province,
Aegean
Sea,
Türkiye.
is
regarded
as
a
marketable
objective
study
at
hand
to
evaluate
statistical
association
weight.
This
evidence
will
be
significant
future
investigations
on
biology
this
species,
which
offer
key
data
about
feeding,
age
growth
species.
Linear
non‐linear
regression
models
were
utilized.
results
obtained
examination
first
address
traits
for
S.
Turkish
marine
waters.
The Science of The Total Environment,
Journal Year:
2024,
Volume and Issue:
942, P. 173656 - 173656
Published: June 1, 2024
Coastal
and
estuarine
environments
are
under
endogenic
exogenic
pressures
jeopardizing
survival
diversity
of
inhabiting
biota.
Information
possible
synergistic
effects
multiple
(a)biotic
stressors
holobiont
interaction
largely
missing
in
estuaries
like
the
Elbe
but
importance
to
estimate
unforeseen
on
animals'
physiology.
Here,
we
seek
leverage
host-transcriptional
RNA-seq
gill
mucus
microbial
16S
rRNA
metabarcoding
data
coupled
with
physiological
abiotic
measurements
a
network
analysis
approach
decipher
impact
health
juvenile
Sander
lucioperca
along
one
largest
European
estuaries.
We
find
mesohaline
areas
characterized
by
tissue
specific
transcriptional
responses
matching
osmosensing
remodeling.
Liver
transcriptomes
instead
emphasized
that
zander
from
highly
turbid
were
undergoing
starvation
which
was
supported
compromised
body
condition.
Potential
pathogenic
bacteria,
including
Shewanella,
Acinetobacter,
Aeromonas
Chryseobacterium,
dominated
microbiome
freshwater
transition
oxygen
minimum
zone.
Their
occurrence
coincided
strong
adaptive
innate
immune
response
host
enhanced
energy
demand
liver
supporting
their
potential
pathogenicity.
Taken
together,
show
fish
species
its
factors
whose
is
expected
increase
consequences
climate
change.
further
present
method
for
close-meshed
detection
main
bacterial
disease
productive
ecosystem.
Animal Microbiome,
Journal Year:
2025,
Volume and Issue:
7(1)
Published: May 6, 2025
The
commercial
demand
for
small
pelagic
fish,
such
as
Atlantic
chub
mackerel
(Scomber
colias),
renders
them
susceptible
to
provenance
fraud.
Scomber
colias
specimens
intestinal
tract
bacteriome
from
five
distinct
fishing
areas
along
the
Portuguese
coastline
were
analyzed
by
4th-generation
sequencing.
Bacteria
diversity
indices
and
differential
abundance
revealed
dissimilarities
in
operational
taxonomic
unit
(OTU)
among
sites.
Random
forest-based
model
yielded
an
85%
accuracy
rate
attributing
sample
based
on
OTU
relative
abundance.
Further
refinement
of
microbial
features
using
Indicator
Species
Analysis,
Linear
Discriminant
Analysis
Effect
Size
(LEfSe)
Gini
scores
enabled
identification
3-5
bacterial
location
biomarkers
per
site.
sequences
linked
pathogenic
bacteria,
particularly
Center-North
Center-South
areas.
While
this
doesn't
imply
active
pathogens,
it
highlights
potential
public
health
concerns
complements
efforts
improve
seafood
microbiological
quality
traceability.
mSystems,
Journal Year:
2023,
Volume and Issue:
8(3)
Published: June 8, 2023
Facultative
marine
bacterial
pathogens
sense
environmental
signals
so
that
the
expression
of
virulence
factors
is
upregulated
on
entry
into
hosts
and
downregulated
during
free-living
lifestyle
in
environment.
In
this
study,
we
utilized
transcriptome
sequencing
to
compare
transcriptional
profiles
Photobacterium
damselae
subsp.
damselae,
a
generalist
pathogen
causes
disease
diverse
animals
fatal
infections
humans
at
NaCl
concentrations
mimic
or
host
internal
milieu,
respectively.
We
here
show
concentration
constitutes
major
regulatory
signal
shapes
uncover
1,808
differentially
expressed
genes
(888
920
response
low-salt
conditions).
Growth
3%
NaCl,
salinity
mimics
lifestyle,
involved
energy
production,
nitrogen
metabolism,
transport
compatible
solutes,
utilization
trehalose
fructose,
carbohydrate
amino
acid
metabolism
with
strong
upregulation
arginine
deiminase
system
(ADS).
addition,
observed
marked
increase
resistance
antibiotics
NaCl.
On
contrary,
low
conditions
(1%
NaCl)
those
encountered
triggered
gene
profile
maximized
production
type
2
secretion
(T2SS)-dependent
cytotoxins
damselysin,
phobalysin
P,
putative
PirAB-like
toxin,
observations
were
corroborated
by
analysis
secretome.
Low
also
iron-acquisition
systems,
efflux
pumps,
other
functions
related
stress
virulence.
The
results
study
greatly
expand
our
knowledge
salinity-responsive
adaptations
versatile
pathogen.
IMPORTANCE
Pathogenic
Vibrionaceae
species
experience
continuous
shifts
their
life
cycles.
However,
impact
changes
regulation
has
been
studied
small
number
Vibrio
species.
analyzed
(Pdd),
facultative
pathogen,
salinity,
demonstrate
growth
1%
comparison
triggers
program
expression,
T2SS-dependent
decrease
bacteria
proposed
constitute
upregulates
genetic
invasion
tissue
damage,
nutrient
scavenging
(notably
iron),
responses.
This
will
surely
inspire
new
research
Pdd
pathobiology,
as
well
important
family
taxa
whose
regulons
still
await
investigation.
Fishes,
Journal Year:
2023,
Volume and Issue:
8(2), P. 60 - 60
Published: Jan. 18, 2023
Infectious
diseases
caused
by
marine
bacterial
pathogens
inflict
increasing
economic
losses
to
fisheries
and
aquaculture,
while
also
posing
a
growing
risk
public
health
affected
species
conservation.
In
this
study,
four
wild
fish
were
collected
at
five
fishing
sites
in
Israel,
divided
into
two
regions—north
(Acre,
Haifa,
Shefayim)
center-south
(Tel-Aviv
Ashdod),
screened
for
Photobacterium
damselae.
An
initial
screening
was
carried
out
using
PCR
analysis
with
specifically
designed
primers
on
DNA
extracted
from
livers
kidneys.
P.
damselae-positive
samples
had
their
16S
rRNA
amplicons
sequenced.
Later,
an
attempt
specify
relevant
sub-species
performed,
three-layered
gene
screen:
Car,
ureC
toxR.
Of
334
samples,
47
(14%)
found
be
damselae-positive,
of
which
20
identified
as
damselae
subsp.
piscicida
(Phdp),
(Phdd)
25
could
not
subspecies.
Our
results
strengthen
the
view
that
residing
polluted
environment
are
receptive
pathogenic
microorganisms.
To
assess
how
presence
may
affect
population
management
conservation,
research
should
followed
studies
aimed
at:
(i)
quantifying
levels
pollutants
pathogen
emergence,
(ii)
creating
standard
pollution-level
index
basis
setting
criteria,
above
authorities
take
measures
precaution.
Aquaculture Reports,
Journal Year:
2023,
Volume and Issue:
32, P. 101693 - 101693
Published: Aug. 3, 2023
Atlantic
sea
scallops,
Placopecten
magellanicus,
are
the
most
economically
important
marine
bivalves
along
northeastern
coast
of
North
America.
Wild
harvest
landings
generate
hundreds
millions
dollars,
and
wild-caught
adults
juvenile
spat
increasingly
being
cultured
in
aquaculture
facilities
coastal
farms.
However,
last
two
weeks
larval
maturation
phase
hatcheries
often
plagued
by
large
mortality
events.
Research
into
other
scallop-
aquacultured-species
point
to
bacterial
infections
or
altered
functionality
microbial
communities
which
associate
with
host.
Despite
intense
filtering
sterilization
seawater,
changing
tank
water
every
48
h,
harmful
microbes
can
still
persist
biofilms
is
high.
There
no
previous
studies
associated
growing
scallop
hatchery
tanks,
nor
wild
scallops.
We
characterized
veliger-stage
larvae,
using
16
S
rRNA
gene
V3-V4
region
sequenced
on
Illumina
MiSeq
platform.
Hatchery
larvae
had
lower
richness
(number
bacteria
taxa
present)
than
biofilms,
a
similar
community
(which
were
both
biofilms.
Bacterial
similarity
between
samples
fluctuated
over
trial
repeated
patterns
rise
fall,
showed
some
correlation
lunar
cycle
that
may
be
proxy
for
effects
spring
tides
trends
seawater
phages
propagated
tanks.
These
results
future
work,
will
inform
methods
increase
survival
these
facilities,
example,
implementing
additional
avoiding
collection
during
tides,
reduce
concern
promote
more
diverse
would
compete
against
pathogens.
International Microbiology,
Journal Year:
2024,
Volume and Issue:
unknown
Published: May 13, 2024
Abstract
The
gills
and
skin
microbiota
microbiome
of
wild
fish
remain
far
more
under-investigated
compared
to
that
farmed
species,
despite
these
animal-microbe
interactions
hold
the
same
ecophysiological
roles
in
both
cases.
In
this
study,
bacterial
profiles
their
presumptive
metabolisms
were
investigated
five
open-sea
fishes:
bullet
tuna
(
Auxis
sp.),
common
dolphinfish
Coryphaena
hippurus
),
Atlantic
little
tunny
Euthynnus
alletteratus
bonito
Sarda
sarda
)
white
marlin
Kajikia
albida
).
Gills
tissues
collected
from
two
three
individuals
per
specimens
caught
by
recreational
trolling
during
summer
2019,
16S
rRNA
gene
diversity
was
analysed
high-throughput
sequencing.
communities
among
species
clearly
different
but
not
microbiota.
dominant
operational
taxonomic
units
belonged
Moraxellaceae,
Pseudomonadaceae,
Rhodobacteraceae,
Staphylococcaceae
Vibrionaceae
families.
Despite
differences
composition,
between
fishes
here
≥
94%
similar
dominated
basic
metabolism,
most
likely
reflecting
continuous
exposure
surrounding
seawater.