Strategies for biofilm optimization of plastic-degrading microorganisms and isolating biofilm formers from plastic-contaminated environments DOI Creative Commons

Adam McFall,

Scott A Coughlin, Gary Hardiman

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 1(1)

Published: Jan. 1, 2024

Abstract The perpetual disposal of plastic waste, combined with ineffective waste management strategies, has resulted in widespread environmental pollution. Microbial biodegradation represents an emerging solution to this problem. However, studies tend overlook the fundamental prerequisite initial surface colonization via biofilm formation. This study had two independent but connected aims relating by microorganisms: enhance formation known degraders, translational potential for improved degradation, and isolate microorganisms from microplastic contaminated environments ability colonize surfaces. Planktonic responses diverse carbon energy sources were investigated over 7 days, using Bacillus subtilis 168, Fusarium solani (Martius) Saccardo, Ideonella sakaiensis 201-F6, Pseudomonas putida KT2440, Rhodococcus ruber C208. enabled optimal conditions each strain be determined. In parallel, samples containing synthetic or natural polymeric substances (anaerobic digestate, landfill leachate, compost) incubated polyethylene terephthalate films, capable colonizing their yielded eight bacterial isolates three genera: Bacillus, Lysinibacillus, Proteus. These genera contain species that have been shown degrade plastics other recalcitrant polymers, demonstrating success our approach. also suggests discrete types may create different ecological niches which can exploited unique colonizers. Our findings underscore importance considering microbial biofilms context biodegradation.

Language: Английский

Plastic pollution and fungal, protozoan, and helminth pathogens – A neglected environmental and public health issue? DOI Creative Commons
Michael J. Ormsby,

Ayorinde Akinbobola,

Richard S. Quilliam

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 882, P. 163093 - 163093

Published: March 28, 2023

Plastic waste is ubiquitous in the environment and can become colonised by distinct microbial biofilm communities, known collectively as 'plastisphere.' The plastisphere facilitate increased survival dissemination of human pathogenic prokaryotes (e.g., bacteria); however, our understanding potential for plastics to harbour disseminate eukaryotic pathogens lacking. Eukaryotic microorganisms are abundant natural environments represent some most important disease-causing agents, responsible tens millions infections, deaths worldwide. While prokaryotic communities terrestrial, freshwater, marine relatively well characterised, such biofilms will also contain species. Here, we critically review fungal, protozoan, helminth associate with plastisphere, consider regulation mechanisms this interaction. As volume continues rise there an urgent need understand role survival, virulence, dissemination, transfer pathogens, effect have on environmental health.

Language: Английский

Citations

20
Structural properties and microbial diversity of the biofilm colonizing plastic substrates in Terra Nova Bay (Antarctica) DOI Creative Commons
Maria Papale, Stefano Fazi,

M. Severini

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 943, P. 173773 - 173773

Published: June 5, 2024

Microbial colonization on plastic polymers has been extensively explored, however the temporal dynamics of biofilm community in Antarctic environments are almost unknown. As a contribute to fill this knowledge gap, structural characteristics and microbial diversity associated with polyvinyl chloride (PVC) polyethylene (PE) panels submerged at 5 m depth collected after 3, 9 12 months were investigated four coastal sites Ross Sea. Additional placed 20 retrieved months. Chemical characterization was performed by FTIR-ATR Raman (through Surface-Enhanced Scattering, SERS) spectroscopy. Bacterial composition quantified single cell level Catalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) Confocal Laser Scanning Microscopy (CLSM); assessed 16S rRNA gene sequencing. This multidisciplinary approach provided new insights into during biofouling process, shedding light succession substrates Significant differences between free-living communities found, more consolidated structured PVC compared PE. Spectral features ascribable tyrosine, polysaccharides, nucleic acids lipids characterized PVC-associated biofilms. Pseudomonadota (among Gammaproteobacteria) Alphaproteobacteria dominated community. Interestingly, Road Bay, close Italian "Mario Zucchelli" research station, growth - already observed summer season, 3 submersion – continued afterwards leading massive abundance end winter (after months). After months, higher percentages Gammaproteobacteria Bay than not-impacted site found. These observations lead us hypothesize that fouling developed first could serve as starter pioneering stimulating successive winter.

Language: Английский

Citations

7
Fungal Diversity and Dynamics during Long-Term Immersion of Conventional and Biodegradable Plastics in the Marine Environment DOI Creative Commons

Aurélie Philippe,

Cyril Noël, B. Eyheraguibel

et al.

Diversity, Journal Year: 2023, Volume and Issue: 15(4), P. 579 - 579

Published: April 20, 2023

Plastics are associated with a worldwide pollution crisis, strong negative impacts on both terrestrial and aquatic ecosystems. In marine environments, various organisms colonizing plastic debris, but few studies have focused fungal communities despite their non-trivial ecological roles in the environment. this study, different types of plastics (biodegradable conventional) immersed natural environments under laboratory controlled settings were collected after long-term colonization. Using metabarcoding approach targeting two genetic markers, namely, ITS2 region V4 hypervariable 18S rRNA gene, we highlighted that polymers distinct from those found surrounding seawater. They also differed significantly between sampling locations nature polymers, indicating colonization was impacted by sites plastics, clear dissimilarities conventional biodegradable polymers. Specifically for PVC polymer (Polyvinyl chloride), observed successive stages biofilm development maturation immersion A noticeable change around 30–40 days settings, suggesting dynamic likely transition formation to biofouling. Overall, study strengthens idea kingdom is an integrated part “plastisphere”.

Language: Английский

Citations

16
Enrichment of native plastic‐associated biofilm communities to enhance polyester degrading activity DOI Creative Commons
Sophie A. Howard, Clodagh M. Carr,

Habteab Isaack Sbahtu

et al.

Environmental Microbiology, Journal Year: 2023, Volume and Issue: 25(12), P. 2698 - 2718

Published: July 28, 2023

Abstract Plastic pollution is an increasing worldwide problem urgently requiring a solution. While recycling rates are globally, only 9% of all plastic waste has been recycled, and with the cost limited downstream uses recycled plastic, alternative needed. Here, we found that expanded polystyrene (EPS) promoted high levels bacterial biofilm formation sought out environmental EPS to characterize these native communities. We demonstrated attached communities had degrading activity. then performed long‐term enrichment experiment where placed robust selection pressure on by limiting carbon availability such was source. Seven resulting enriched increased activity compared starting Pseudomonas stutzeri predominantly identified in six seven as strongest polyester degrader. Sequencing one isolate P. revealed two putative polyesterases MHETase. This indicates plastic‐associated biofilms source for bacteria have plastic‐degrading potential, this potential can be unlocked through selective further vitro experiments, biodegradative better than nature.

Language: Английский

Citations

15
Plastisphere composition in a subtropical estuary: Influence of season, incubation time and polymer type on plastic biofouling DOI
Tobias Sérvulo, John D. Taylor, Maíra Proietti

et al.

Environmental Pollution, Journal Year: 2023, Volume and Issue: 332, P. 121873 - 121873

Published: May 25, 2023

Language: Английский

Citations

14
Short‐term plastisphere colonization dynamics across six plastic types DOI Creative Commons
Vinuri Silva, Vilma Pérez, Bronwyn M. Gillanders

et al.

Environmental Microbiology, Journal Year: 2023, Volume and Issue: 25(12), P. 2732 - 2745

Published: June 21, 2023

Abstract Marine plastic pollution is a major concern worldwide, but the understanding of plastisphere dynamics remains limited in southern hemisphere. To address this knowledge gap, we conducted study South Australia to investigate prokaryotic community and its temporal changes over 4 weeks. We submerged six types (i.e., High‐Density Polyethylene [HDPE], Polyvinyl chloride [PVC], Low‐Density [LDPE], Polypropylene [PP], Polystyrene [PS] understudied textile, polyester [PET]) wood seawater sampled them weekly characterize using 16S rRNA gene metabarcoding. Our results showed that composition shifted significantly short time scales weeks), each type had distinct groups unique genera. In particular, PVC was dominated by Cellvibrionaceae taxa, distinguishing it from other plastics. Additionally, textile polyester, which rarely studied research, supported growth group 25 genera (which included potential pathogenic Legionella genus). Overall, provides valuable insights into colonization contributes narrowing research gap on hemisphere plastisphere.

Language: Английский

Citations

12
Comparative assessment of microplastic ingestion among deep sea decapods: distribution analysis in Sardinian and Catalan waters DOI

L Chiacchio,

Alessandro Cau, Anna Soler‐Membrives

et al.

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 120962 - 120962

Published: Jan. 1, 2025

Language: Английский

Citations

0
Putative Plastic Degrading Communities within New Zealand's Geothermal Environments DOI

Nikolai Pavlov,

Jessica A. Wallbank, Syrie M. Hermans

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Putative Plastic Degrading Communities within New Zealand’s Geothermal Environments DOI Creative Commons
Н.В. Павлов, Jessica A. Wallbank, Sabine Hermans

et al.

Published: May 1, 2025

Language: Английский

Citations

0
Microbial colonization patterns and biodegradation of petrochemical and biodegradable plastics in lake waters: insights from a field experiment DOI Creative Commons
Francesca Di Pippo, V. Bocci, Stefano Amalfitano

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 6, 2023

Once dispersed in water, plastic materials become promptly colonized by biofilm-forming microorganisms, commonly known as plastisphere.By combining DNA sequencing and Confocal Laser Scanning Microscopy (CLSM), we investigated the plastisphere colonization patterns following exposure to natural lake waters (up 77 days) of either petrochemical or biodegradable (low density polyethylene - LDPE, terephthalate PET, polylactic acid PLA, starch-based MaterBi® Mb) comparison planktonic community composition. Chemical composition, water wettability, morphology surfaces were evaluated, through Transform Infrared Spectroscopy (ATR-FTIR), Electron (SEM), static contact angle analysis, assess possible effects microbial biodegradation activity.The phylogenetic composition communities was notably different. Pioneering colonisers, likely selected from waters, found associated with all materials, along a core more than 30 abundant bacterial families polymers. The different derived hydrocarbons (i.e., LDPE PET) (PLA Mb), used opportunistic aquatic microorganisms adhesion rather carbon sources. Mb-associated (i.e. mostly members family Burkholderiaceae) able degrade starch residues on polymer surfaces, although Mb matrix maintained its original chemical structure morphology. Overall, our findings provide insights into complex interactions between highlighting importance understanding dynamics better manage fate debris environment.

Language: Английский

Citations

10