Insights in Pharmaceutical Pollution: The Prospective Role of eDNA Metabarcoding

Toxics, Journal Year: 2023, Volume and Issue: 11(11), P. 903 - 903

Published: Nov. 5, 2023

Environmental pollution is a growing threat to natural ecosystems and one of the world’s most pressing concerns. The increasing worldwide use pharmaceuticals has elevated their status as significant emerging contaminants. Pharmaceuticals enter aquatic environments through multiple pathways related anthropogenic activity. Their high consumption, insufficient waste treatment, incapacity organisms completely metabolize them contribute accumulation in environments, posing all life forms. Various analytical methods have been used quantify pharmaceuticals. Biotechnology advancements based on next-generation sequencing (NGS) techniques, like eDNA metabarcoding, enabled development new for assessing monitoring ecotoxicological effects metabarcoding valuable biomonitoring tool pharmaceutical because it (a) provides an efficient method assess predict status, (b) identifies sources, (c) tracks changes levels over time, (d) assesses ecological impact pollution, (e) helps prioritize cleanup mitigation efforts, (f) offers insights into diversity composition microbial other bioindicator communities. This review highlights issue while emphasizing importance using modern NGS-based actions its environmental more consistently effectively.

Language: Английский

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eDNA-based seasonal monitoring reveals fish diversity patterns in mangrove habitats of Guinea-Bissau

Regional Studies in Marine Science, Journal Year: 2025, Volume and Issue: unknown, P. 104013 - 104013

Published: Jan. 1, 2025

Language: Английский

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Environmental DNA and its role in identifying yeast biodiversity in mangrove ecosystem: A literature review

E3S Web of Conferences, Journal Year: 2025, Volume and Issue: 605, P. 03005 - 03005

Published: Jan. 1, 2025

Yeast biodiversity in mangrove ecosystems is crucial for their health and ecological functions. However, traditional methods often fail to detect yeast species that are difficult culture or slow growing, leaving a significant portion of this undetected. This study explores the use environmental DNA (eDNA) as an alternative method identifying ecosystems, addressing data gaps left by conventional methods. Data collected from various relevant literature sources, including scientific journals, books, research reports. The review reveals eDNA analysis can improve conservation management providing robust assessments. Future should focus on understanding persistence, implications, improving bioinformatics better interpretation. In-depth long-term studies recommended temporal spatial dynamics communities ecosystems. Further validation conditions needed ensure reliability. Research interactions with other ecosystem components impact human activities like pollution habitat change also important.

Language: Английский

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Habitat management and restoration as missing pieces in flats ecosystems conservation and the fishes and fisheries that they support

Fisheries, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

ABSTRACT Flats ecosystems are dynamic, shallow, nearshore marine environments that interconnected and provide immense ecological socio-economic benefits. These habitats support a diversity of fish populations various fisheries, yet they increasingly threatened by anthropogenic stressors, including overfishing, habitat degradation, coastal development, the cascading effects climate change. Effective management restoration essential but often missing for flats ecosystems. Despite navigating landscape imperfect knowledge these systems, decisive action implementation protection is currently needed through policy practice. We present comprehensive set 10 strategic guiding principles necessary integrating conservation flat include calls ecosystem-based ­management, adaptive strategies leverage diverse partnerships, scientific research, legislative initiatives, local traditional knowledge. Drawing on successes in other environmental realms, we emphasize importance evidence-informed approaches to address complexities uncertainties aim advance restoration, promoting integrity strengthening resilience important environments.

Language: Английский

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Aquatic eDNA outperforms sedimentary eDNA for the detection of estuarine fish communities in subtropical coastal vegetated ecosystems

Journal of Fish Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Abstract In South Africa, mangrove forests and seagrass meadows often co‐occur in estuarine systems combination host rich, endemic biodiversity. There is an urgent need to explore community diversity coastal vegetated ecosystems, given the degrading effects of climate change anthropogenic pressures. Environmental DNA metabarcoding a promising biomonitoring tool African although studies highlight optimise standardise sampling protocols. This study aimed contribute developing protocols by understanding applicability environmental (eDNA) within habitats investigating using two different sample types. Aquatic sedimentary samples from three estuaries along east coast with MiFish metabarcoding, detected 148 operational taxonomic units (OTUs) representing 67 fish genera 50 families, only 16% were resolved species. We observed that aquatic eDNA 97% total diversity, suggesting this efficient comprehensively detect ichthyofaunal diversity. did not communities between mangroves seagrasses our findings underscore importance taking hydrological connectivity into consideration for design. Overall, work reinforces key considerations future focused on fauna estuaries, therefore contributes optimising support

Language: Английский

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Metazoan diversity and community assemblages in sediments across a Western Pacific Trench-Arc-Basin system: insights from eDNA metabarcoding

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 10

Published: Jan. 17, 2024

Trench-arc-basin (TAB) systems are widely distributed in the deep sea, yet our understanding of their biodiversity patterns and community assemblages remains limited. In this study, we collected sediment samples from 22 stations across a Western Pacific TAB system identified 85 families marine benthos 15 phyla by using eDNA metabarcoding with 18S rRNA gene V4 region. Nematodes were most dominant metazoan taxa followed echinoderms, arthropods, annelids. The highest species specificity observed at located near seamounts. highly heterogeneous system, likely induced large geographic barriers high habitat heterogeneity. Furthermore, total organic carbon content median grain size drive overall composition, water depth exerts significant influence on richness abundance. Our results provide insight into diversity distribution data for further comparisons modeling studies.

Language: Английский

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Exploring fish communities in the mud volcano polluted river using environmental DNA metabarcoding

Environmental Advances, Journal Year: 2024, Volume and Issue: 16, P. 100534 - 100534

Published: April 14, 2024

In ecosystems subjected to environmental disturbances such as mud volcanoes, understanding the dynamics of fish communities is essential for ecological assessment and conservation. This study employs DNA (eDNA) metabarcoding first time explore within a challenging unique environment—a volcano-polluted river. High-throughput sequencing was utilized assess alpha beta diversity, revealing distinct patterns in community composition response discharge activity. The results indicate that affected sites exhibit remarkably different during active inactive discharge, with impact extending downstream river mouth. Notably, application eDNA identified vulnerable species, Rasbora lateristriata, emphasizing its utility detecting high-risk species. Despite challenges species identification reference databases, use longer 16S rRNA fragments offered enhanced taxonomic resolution interspecific sequence variation. approach showcases potential assessing diversity polluted aquatic ecosystems, significance biodiversity surveys monitoring. underscores value an efficient comprehensive tool studying freshwater highlights need expanding databases address genetic coverage gaps, particularly less-studied environments.

Language: Английский

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Application of eDNA Metabarcoding in the Assessment of Fish Biodiversity in Philippine Mangroves: Challenges and Opportunities

Regional Studies in Marine Science, Journal Year: 2024, Volume and Issue: 77, P. 103642 - 103642

Published: June 24, 2024

Biodiversity assessments are important in designing mangrove conservation and restoration programs. In the Philippines, conventional biodiversity assessment methods (e.g., trap nets, fish visual census) can be time-consuming, labor-intensive, expensive. recent years, environmental DNA (eDNA) metabarcoding has been an emerging tool for rapid monitoring as it is fast, non-intrusive, provide broader detection of fauna. But, still subject to various field sampling laboratory analysis constraints. Here, we applied eDNA method document assess mangroves from two biogeographic regions Philippines: Oriental Mindoro West Philippine Sea Sorsogon Northern Sea. Using 12 S genetic markers water samples, detected 89 species 44 families. Only twelve were commonly both sites. Several found Philippine's list economically aquatic organisms while one (Epinephelus fuscoguttatus) was classified Vulnerable IUCN's Red List Threatened Species. Seventy-six percent more probably because sites within a seascape mangroves, seagrasses, coral reefs. contrast, lesser could limited points coastal fringes. Our results serve baseline data first obtained using this country. However, observed some limitations that should addressed improve method: (1) lack or absence comprehensive reference database specific organisms; (2) low reads which attributed insufficient on-site filtration due turbid seawaters common mangroves; (3) possible cross-contaminations affect comparative analyses. Despite these limitations, able demonstrate usefulness technique doing address knowledge gaps studies contribute its

Language: Английский

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Advancing River Health Assessments: Integrating Microscopy and Molecular Techniques through Diatom Indices

Water, Journal Year: 2024, Volume and Issue: 16(6), P. 853 - 853

Published: March 15, 2024

This paper reviews the evolution and integration of diatom-based water quality assessments with environmental DNA (eDNA) techniques for advancing river ecosystem health evaluations. Traditional methods, relying on microscopy diatom indices, have significantly contributed to our understanding aquatic ecosystems but face challenges such as need taxonomic expertise labor-intensive nature sample collection. Recent advancements in molecular biology, particularly eDNA analysis, offer opportunities overcome these limitations, providing more accurate comprehensive assessments. study highlights benefits combining traditional modern techniques, enhancing precision efficiency By addressing standardizing methods improving species identification through reference libraries advanced bioinformatics tools, this integrated approach aims refine advance effectiveness strategies monitoring managing amidst changes.

Language: Английский

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Unveiling cryptic macroinvertebrate sentinels to enhance biomonitoring in tropical rivers: Bridging traditional approaches with DNA barcoding in the Indo-Burma biodiversity hotspot

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 958, P. 178064 - 178064

Published: Dec. 17, 2024

Human activities present significant threats to tropical freshwater ecosystems, notably in many global biodiversity hotspots, that are further increased by inadequate taxonomic knowledge and the lack of appropriate biomonitoring tools. This study integrates globally validated approaches with DNA-based identification methods create a macroinvertebrate-based tool for diagnosing ecosystem health assessing river ecosystems Myanmar (Indo-Burma bioregion). To evaluate site degradation, comprehensive data on water habitat quality, as well land use information, were collected. Riverine macroinvertebrates sampled kick sampling, subsequent DNA barcoding analysis was used establish molecular units (MTUs) key bioindicator groups, including Ephemeroptera, Plecoptera, Trichoptera, Coleoptera, Odonata (EPTCO) species-level nomenclature lacking. Tolerance scores local fauna derived along an environmental degradation gradient enable comparisons widely adopted assessment tools relying macroinvertebrate metrics. In both areas, upper parts networks generally undisturbed human while stressors associated urban agricultural evident lower catchments. The highest precision found when establishing tolerance adjusted species composition each area separately. Although family-level-based multimetric approach significantly related main gradient, assessments utilizing cryptic emerged being most precise indicator areas. Our highlights synergistic benefits merging traditional ecosystems. halt decline curb extent escalating nature crisis, such integrated will be highly valuable understudied biodiversity-rich aquatic

Language: Английский

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