Abundance of Oligoflexales bacteria is associated with algal symbiont density independent of thermal stress in Aiptasia anemones DOI Creative Commons
Emily G. Aguirre,

Marissa J. Fine,

Carly D. Kenkel

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 17, 2023

ABSTRACT Many multicellular organisms, such as humans, plants, and invertebrates, depend on symbioses with microbes for metabolic cooperation exchange. Reef-building corals, an ecologically important order of are particularly vulnerable to environmental stress in part because their nutritive symbiosis dinoflagellate algae, yet also benefit from these other microbial associations. While coral microbiomes remain difficult study complexity, the anemone Aiptasia is emerging a simplified model. Research has demonstrated co-occurrences between microbiome composition abundance type algal symbionts cnidarians. However, whether patterns result general stress-induced shifts or depletions algal-associated bacteria remains unclear. Our aimed distinguish effect changes symbiont density thermal symbiotic strain CC7 by comparing them aposymbiotic anemones, depleted native symbiont, Symbiodinium linucheae . analysis indicated that overall, had greatest impact disrupting microbiome. We found three bacterial classes made up most relative (60-85 %) all samples, but rare fluctuated states following stress. observed S. correlated Oligoflexales, suggesting may be primary algae. The findings this help expand knowledge prospective multipartite cnidarian holobiont how they respond disturbance.

Language: Английский

Microbiome origin and stress-related changes in bacterial abundance of the photosymbiotic sea slug Berghia stephanieae (Á. Valdés, 2005) DOI Creative Commons
Corinna Sickinger,

Sofie Marie Brackwehr,

Jenny Melo Clavijo

et al.

Symbiosis, Journal Year: 2024, Volume and Issue: 93(2), P. 177 - 192

Published: June 1, 2024

Abstract The precise mechanisms that allow animals and phototrophic organisms to form a stable photosymbiotic relationship are still unknown. While previous studies focused on genomic adaptations of the animal host, more recent research looked into role bacteria in photosymbiosis. Here, we analyzed core microbiome sea slug Berghia stephanieae its food source Exaiptasia diaphana understand if linked bacterial metabolic pathways differ between unstable This feeds solely model cnidarian E. steals their photobionts which can only maintain for week. We additionally examined influence light starvation stress slug’s composition, common experimental set-ups elucidate slugs. Our results show B. differed significantly suggesting is not obtained from or water column indicates vertical transmission. Further, differences imply does support photosymbiosis due an insufficient nitrogen cycle part photobiont. Starving slugs induced shift towards increased abundance potential pathogens led downregulation sulphur cycle. Yet, darkness resulted depletion most switch toward fixation. emphasizes different holobiont members contribute essential nutrient cycles, it important look beyond photobiont holobiont.

Language: Английский

Citations

1
Abundance of Oligoflexales bacteria is associated with algal symbiont density, independent of thermal stress in Aiptasia anemones DOI Creative Commons
Emily G. Aguirre,

Marissa J. Fine,

Carly D. Kenkel

et al.

Ecology and Evolution, Journal Year: 2023, Volume and Issue: 13(12)

Published: Dec. 1, 2023

Abstract Many multicellular organisms, such as humans, plants, and invertebrates, depend on symbioses with microbes for metabolic cooperation exchange. Reef‐building corals, an ecologically important order of are particularly vulnerable to environmental stress in part because their nutritive symbiosis dinoflagellate algae, yet also benefit from these other microbial associations. While coral microbiomes remain difficult study complexity, the anemone Aiptasia is emerging a simplified model. Research has demonstrated co‐occurrences between microbiome composition abundance type algal symbionts cnidarians. However, whether patterns result general stress‐induced shifts or depletions algal‐associated bacteria remains unclear. Our aimed distinguish effect changes symbiont density thermal symbiotic strain CC7 by comparing them aposymbiotic anemones, depleted native symbiont, Symbiodinium linucheae . analysis indicated that overall had greatest impact disrupting microbiome. We found three bacterial classes made up most relative (60%–85%) all samples, but rare fluctuated states following stress. observed S. correlated Oligoflexales, suggesting may be primary algae. The findings this help expand knowledge prospective multipartite cnidarian holobiont how they respond disturbance.

Language: Английский

Citations

3
The bacterial microbiome of symbiotic and menthol-bleached polyps of long-term aquarium-reared Galaxea fascicularis DOI Creative Commons
Giulia Puntin, Jane C. Y. Wong, Till Röthig

et al.

Peer Community Journal, Journal Year: 2024, Volume and Issue: 4

Published: June 27, 2024

Coral reefs support the livelihood of half a billion people but are at high risk collapse due to vulnerability corals climate change and local anthropogenic stressors. While understanding coral functioning is essential guide conservation efforts, research challenged by complex nature corals. They exist as metaorganisms (holobionts), constituted association between (coral) animal host, its obligate endosymbiotic algae (Symbiodiniaceae), other microorganisms comprising bacteria, viruses, archaea, fungi protists. Researchers therefore increasingly turn model organisms unravel holobiont complexity, dynamics, how these determine health fitness The Galaxea fascicularis an emerging organism for symbiosis with demonstrated suitability aquarium rearing reproduction, manipulation host-Symbiodiniaceae symbiosis. However, little known about response G. microbiome menthol bleaching—the experimental removal Symbiodiniaceae which represents first step in coral-algal manipulation. For this, we characterized bacterial symbiotic menthol-bleached originating from Red Sea South China (Hong Kong) that were long-term aquarium-reared separate facilities. We found coral-associated microbiomes composed relatively few taxa (10-78 ASVs). Symbiotic polyps (clonal replicates) same colony had similar microbiomes, distinct those colonies despite co-culturing shared aquaria. A pattern seemingly differential bleaching two facilities emerged, warranting further investigation into role conditions. Nevertheless, changes community composition overall appeared be stochastic suggesting dysbiotic state. Considering importance treatment captive research, our results—although preliminary—contribute fundamental knowledge development research.

Language: Английский

Citations

0
Abundance of Oligoflexales bacteria is associated with algal symbiont density independent of thermal stress in Aiptasia anemones DOI Creative Commons
Emily G. Aguirre,

Marissa J. Fine,

Carly D. Kenkel

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 17, 2023

ABSTRACT Many multicellular organisms, such as humans, plants, and invertebrates, depend on symbioses with microbes for metabolic cooperation exchange. Reef-building corals, an ecologically important order of are particularly vulnerable to environmental stress in part because their nutritive symbiosis dinoflagellate algae, yet also benefit from these other microbial associations. While coral microbiomes remain difficult study complexity, the anemone Aiptasia is emerging a simplified model. Research has demonstrated co-occurrences between microbiome composition abundance type algal symbionts cnidarians. However, whether patterns result general stress-induced shifts or depletions algal-associated bacteria remains unclear. Our aimed distinguish effect changes symbiont density thermal symbiotic strain CC7 by comparing them aposymbiotic anemones, depleted native symbiont, Symbiodinium linucheae . analysis indicated that overall, had greatest impact disrupting microbiome. We found three bacterial classes made up most relative (60-85 %) all samples, but rare fluctuated states following stress. observed S. correlated Oligoflexales, suggesting may be primary algae. The findings this help expand knowledge prospective multipartite cnidarian holobiont how they respond disturbance.

Language: Английский

Citations

1