Investigation of the mechanisms involved in the biocontrol activities of natural products from a marine soil bacterium against rice blast DOI Creative Commons
Liwang Fei, Rahila Hafeez, Junliang Zhang

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Abstract BACKGROUND Rice blast, caused by Pyricularia oryzae , is a devastating fungal disease threatening global rice production. Overreliance on chemical fungicides has raised environmental concerns and led to resistant strains, necessitating the development of sustainable alternatives. This study integrated marine microbiology natural antifungal compounds create eco‐friendly alternatives for management. RESULTS We identified Pseudomonas aeruginosa R64 with broad‐spectrum antimicrobial activity from mangrove soil in Mai Po Nature Reserve. The fermentation extract (RFE) exhibited multifaceted inhibition P. suppressing mycelial growth, conidiation, conidial germination appressorial formation, while disturbing cell wall membrane function. It also attenuated virulence impairing penetration invasive growth. Further analysis phenazines quinolines as primary RFE, corroborated PCR detection corresponding phenazine biosynthetic gene clusters. Comparative bioassays two main bioactive components phenazine‐1‐carboxamide (PCN) phenazine‐1‐carboxylic acid (PCA), against implicated PCN principal effector. RFE had higher efficacy than tricyclazole growth inhibition, but were less effective isoprothiolane. Furthermore, displayed lower acute ecotoxicity an indicator organism isoprothiolane, suggesting their potential biopesticides blast CONCLUSION Natural products bacterium inhibited key developmental infection processes effectively reducing development. promising low mangrove‐associated bacteria highlight untapped innovative, fungicide mining agriculture. © 2025 Author(s). Pest Management Science published John Wiley & Sons Ltd behalf Society Chemical Industry.

Language: Английский

Investigation of the mechanisms involved in the biocontrol activities of natural products from a marine soil bacterium against rice blast DOI Creative Commons
Liwang Fei, Rahila Hafeez, Junliang Zhang

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Abstract BACKGROUND Rice blast, caused by Pyricularia oryzae , is a devastating fungal disease threatening global rice production. Overreliance on chemical fungicides has raised environmental concerns and led to resistant strains, necessitating the development of sustainable alternatives. This study integrated marine microbiology natural antifungal compounds create eco‐friendly alternatives for management. RESULTS We identified Pseudomonas aeruginosa R64 with broad‐spectrum antimicrobial activity from mangrove soil in Mai Po Nature Reserve. The fermentation extract (RFE) exhibited multifaceted inhibition P. suppressing mycelial growth, conidiation, conidial germination appressorial formation, while disturbing cell wall membrane function. It also attenuated virulence impairing penetration invasive growth. Further analysis phenazines quinolines as primary RFE, corroborated PCR detection corresponding phenazine biosynthetic gene clusters. Comparative bioassays two main bioactive components phenazine‐1‐carboxamide (PCN) phenazine‐1‐carboxylic acid (PCA), against implicated PCN principal effector. RFE had higher efficacy than tricyclazole growth inhibition, but were less effective isoprothiolane. Furthermore, displayed lower acute ecotoxicity an indicator organism isoprothiolane, suggesting their potential biopesticides blast CONCLUSION Natural products bacterium inhibited key developmental infection processes effectively reducing development. promising low mangrove‐associated bacteria highlight untapped innovative, fungicide mining agriculture. © 2025 Author(s). Pest Management Science published John Wiley & Sons Ltd behalf Society Chemical Industry.

Language: Английский

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