Discovery of Peptidic Siderophore Degradation by Screening Natural Product Profiles in Marine-Derived Bacterial Mono- and Cocultures

Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Coral reefs are hotspots of marine biodiversity, which results in the synthesis a wide variety compounds with unique molecular scaffolds, and bioactivities, rendering an ecosystem interest. The chemodiversity stems from intricate relationships between inhabitants reef, as chemistry produced partakes intra- interspecies communication, settlement, nutrient acquisition, defense. However, coral declining at unprecedented rate due to climate change, pollution, increased incidence pathogenic diseases. Among pathogens, Vibrio spp. bacteria key players resulting high mortality. Thus, alternative strategies such application beneficial isolated disease-resilient species being explored lower burden species. Here, we apply coculturing coral-derived leverage recent advancements untargeted metabolomics discover engineerable traits. By chasing chemical change coculture, report Microbulbifer spp.-mediated degradation amphibactins, by sequester iron. Additional biochemical experiments revealed that occurs peptide backbone requires enzyme fraction Microbulbifer. A reduction iron affinity is expected loss one Fe(III) binding moiety. Therefore, hypothesize this shapes community behaviors it pertains limiting environment, survival. Furthermore, sp. suppressed natural product bacteria. Understanding mechanisms behind these interactions will enable engineering probiotic capable lowering burdens during heat waves disease.

Language: Английский

---

When Microbial Interactions Go Wrong: Coral Bleaching, Disease, and Dysbiosis

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 169 - 180

Published: Jan. 1, 2025

Language: Английский

---

Ecosystem Microbiology of Coral Reefs

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 131 - 144

Published: Jan. 1, 2025

Language: Английский

---

Novel metagenomics analysis of stony coral tissue loss disease

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 3, 2024

Stony coral tissue loss disease (SCTLD) has devastated reefs off the coast of Florida and continues to spread throughout Caribbean. Although a number bacterial taxa have consistently been associated with SCTLD, no pathogen definitively implicated in etiology SCTLD. Previous studies predominantly focused on prokaryotic community through 16S rRNA sequencing healthy affected tissues. Here, we provide different analytical approach by applying bioinformatics pipeline publicly available metagenomic samples SCTLD lesions tissues from four stony species. To compensate for lack reference genomes, used data apparently approximate host genome microbiome reference. These reads were then as which matched removed diseased lesion samples, remaining only taxonomically classified at DNA protein levels. For classifications, identification protocol originally designed identify pathogens human fast sequence aligner. assess utility our pipeline, species-level analysis candidate genus,

Language: Английский

---

Trade-off between photosymbiosis and innate immunity influences cnidarian’s response to pathogenic bacteria

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 291(2032)

Published: Oct. 1, 2024

Mutualistic relationships with photosynthetic organisms are common in cnidarians, which form an intracellular symbiosis dinoflagellates the family Symbiodiniaceae. The establishment and maintenance of these symbionts associated suppression key host immune factors. Because this, there potential trade-offs between nutrition that cnidarian hosts gain from their ability to successfully defend themselves pathogens. To investigate trade-offs, we utilized facultatively symbiotic polyps upside-down jellyfish Cassiopea xamachana exposed aposymbiotic pathogen Serratia marcescens . Symbiotic had a lower probability survival following S. exposure. Gene expression analyses 24 hours exposure indicate animals mounted more damaging response, higher levels inflammation oxidative stress likely resulting severe disruptions cellular homeostasis. Underlying this response may be differences constitutive pathogen-induced transcription factors rather than broadscale during symbiosis. Our findings polyps, hosting limits C. xamachana’ s survive exposure, indicating trade-off immunity has implications for coral disease research.

Language: Английский

---

Novel metagenomics analysis of stony coral tissue loss disease

G3 Genes Genomes Genetics, Journal Year: 2024, Volume and Issue: 14(8)

Published: June 20, 2024

Abstract Stony coral tissue loss disease (SCTLD) has devastated reefs off the coast of Florida and continues to spread throughout Caribbean. Although a number bacterial taxa have consistently been associated with SCTLD, no pathogen definitively implicated in etiology SCTLD. Previous studies predominantly focused on prokaryotic community through 16S rRNA sequencing healthy affected tissues. Here, we provide different analytical approach by applying bioinformatics pipeline publicly available metagenomic samples SCTLD lesions tissues from 4 stony species. To compensate for lack reference genomes, used data apparently approximate host genome microbiome reference. These reads were then as which matched removed diseased lesion samples, remaining only taxonomically classified at DNA protein levels. For classifications, identification protocol originally designed identify pathogens human fast sequence aligner. assess utility our pipeline, species-level analysis candidate genus, Vibrio, was demonstrate pipeline's effectiveness. Our revealed both complementary unique members compared prior metagenome same dataset.

Language: Английский

---

Machine Learning Approaches for Classifying and Characterizing Coral Diseases

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 19, 2024

Background: Anthropogenic climate change has had devastating effects on the Florida and Caribbean reef systems, primarily due to increased disease outbreaks. Climate contributes rising frequency of marine diseases by expanding pathogen ranges heightening host susceptibility environmental stress. Specifically, there been a stark rise in events targeting multiple coral species, resulting high mortality rates declining biodiversity. Although many these present similar visual symptoms, they exhibit varying require distinct treatment protocols. Advances transcriptomics research have enhanced our understanding responses different diseases, but more sophisticated methods are required classify that appear visually similar. Results: This study provides first machine learning algorithm can two common diseases: stony tissue loss (SCTLD) white plague (WP). This also identifies 463 biomarkers, with 275 unique SCTLD 167 WP. These biomarkers highlight differences immune algorithms were tested validated samples collected in situ, supporting biomarker efficacy identified for classification. The final model was built partial least squares discriminant analysis highly predictive an AUC 0.9895 low error rates. Conclusion: study provides diagnostic tool reliably distinguishes between phenotypically provide characterizations

Language: Английский

---

Assessment of nutrient amendments on stony coral tissue loss disease in Southeast Florida

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: Oct. 10, 2024

Florida’s coral reefs are facing a multi-year outbreak of stony tissue loss disease (SCTLD) with dramatic consequences for communities. However, potential anthropogenic and environmental drivers SCTLD progression severity remain poorly understood. This study was designed to determine the impacts nutrient amendments on spread in Southeast Florida. In situ fertilizer 30 g Osmocote™ slow-release were used mimic effects agricultural urban runoff. Fifteen healthy thirty SCTLD-affected Montastraea cavernosa colonies tagged divided into three experimental groups: apparently colonies, nutrient-amended control colonies. lesion progression, loss, prevalence tracked over 30-day amendment period an additional 40 days after removal if there any latent or recovery effects. Coral time using Structure-from-Motion photogrammetry recorded from diver surveys within 3-m radius surrounding five centroid each group. Throughout experiment, temperature, concentrations, status also monitored. Ultimately, we observed no significant differences between groups, suggesting that had effect during this experiment

Language: Английский

---

Metabolomic profiles of stony coral species from the Dry Tortugas National Park display inter- and intraspecies variation

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 12, 2024

Abstract Coral reefs are experiencing unprecedented loss in coral cover due to increased incidence of disease and bleaching events. Thus, understanding mechanisms susceptibility resilience, which vary by species, is important. In this regard, untargeted metabolomics serves as an important hypothesis-building tool enabling delineation molecular factors underlying or resilience. study, we characterize metabolomes four species visually healthy stony corals, including Meandrina meandrites , Orbicella faveolata Colpophyllia natans Montastraea cavernosa collected at least a year before tissue reached the Dry Tortugas, Florida demonstrate that both symbiont host-derived biochemical pathways species. Metabolomes displayed minimal intraspecies variability highest biological activity against pathogens when compared other study. Application advanced metabolite annotation methods enabled several interspecies variability. Specifically, endosymbiont-derived vitamin E family compounds, betaine lipids, acylcarnitines were among top predictors Since features contributed inter- variation synthesized endosymbiotic Symbiodiniaceae, could be major source these compounds our data will guide further investigations into Symbiodiniaceae-derived pathways. Importance. Previous research profiling gene expression, proteins, metabolites produced during thermal stress has reported importance resistance. However, corals their role diseases limited. We surveyed with differing susceptibilities devastating applied approaches determine host Using approach, propose survey immune markers such acylcarnitines, infer resilience diseases. As time-resolved multi-omics datasets generated for disease-impacted approach findings valuable providing insight

Language: Английский

---

Two methodologies and timescales show no promotion of antibiotic resistance from in-water coral disease treatments

Published: Nov. 1, 2024

Abstract The decimation of reefs caused by stony coral tissue loss disease prompted the use a topical amoxicillin treatment to prevent mortality. Application this led concerns about unintentional impacts such as potential alteration microbiome and possible spread antibiotic resistance. We used two different methodologies – microbial RNA sequencing qPCR array assess these concerns. was conducted on mucus samples collected before 24 hours after application wild Montastraea cavernosa . No differences in expression resistance genes (ARGs) were detected. Additionally, there no notable changes communities between samples. Microbial ARGs over longer timescales Colpophylia natans , comparing never-treated corals with ones treated single time seven months prior those multiple times more prior. clinically relevant represented arrays detected across any A small number above-detection reads (4 corals, 2 once-treated 0 multi-treated corals) may indicate weak amplification similar environmental (non-anthropogenic) corals. Results that localized mortality SCTLD-affected is neither disrupting nor driving proliferation

Language: Английский

---