Dielectrophoresis for Isolating Low-Abundance Bacteria Obscured by Impurities in Environmental Samples DOI Creative Commons
Jaeyoung Yu, Hajime Yuasa, Ikuo Hırono

et al.

Marine Biotechnology, Journal Year: 2025, Volume and Issue: 27(2)

Published: March 14, 2025

Abstract Bacterial communities associated with living organisms play critical roles in maintaining health and ecological balance. While dominant bacteria have been widely studied, understanding the role of low-abundance has become increasingly important due to their unique roles, such as regulating bacterial community dynamics supporting host-specific functions. However, detecting these remains challenging, impurities environmental samples mask signals compromise accuracy analyses. This study explored use dielectrophoresis (DEP) a practical approach isolate obscured by impurities, comparing its utility conventional centrifugation methods. Using two shrimp species, Neocaridina denticulata Penaeus japonicus , DEP effectively isolated fractions while reducing enabling detection undetected centrifuged samples. These newly detected were potentially linked diverse functions, nutrient cycling immune modulation, highlighting highly potential support host-microbial interactions. Overall, we believe that offers solution for overlooked methods exploring diversity functional contributions aquaculture biotechnology.

Language: Английский

Dielectrophoresis for Isolating Low-Abundance Bacteria Obscured by Impurities in Environmental Samples DOI Creative Commons
Jaeyoung Yu, Hajime Yuasa, Ikuo Hırono

et al.

Marine Biotechnology, Journal Year: 2025, Volume and Issue: 27(2)

Published: March 14, 2025

Abstract Bacterial communities associated with living organisms play critical roles in maintaining health and ecological balance. While dominant bacteria have been widely studied, understanding the role of low-abundance has become increasingly important due to their unique roles, such as regulating bacterial community dynamics supporting host-specific functions. However, detecting these remains challenging, impurities environmental samples mask signals compromise accuracy analyses. This study explored use dielectrophoresis (DEP) a practical approach isolate obscured by impurities, comparing its utility conventional centrifugation methods. Using two shrimp species, Neocaridina denticulata Penaeus japonicus , DEP effectively isolated fractions while reducing enabling detection undetected centrifuged samples. These newly detected were potentially linked diverse functions, nutrient cycling immune modulation, highlighting highly potential support host-microbial interactions. Overall, we believe that offers solution for overlooked methods exploring diversity functional contributions aquaculture biotechnology.

Language: Английский

Citations

0