International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1703 - 1703
Published: Feb. 17, 2025
Migratory fish often encounter hypoxic zones during migration, which can lead to varying degrees of stress. This issue has become increasingly severe due human activities and climate change, have resulted in the expansion aquatic environments. However, there is limited research on how these species respond stress subsequent recovery. In this study, we used Eleutheronema tetradactylum, a well-recognized migratory economically valuable species, as model organism. Histological analysis revealed extensive neuronal damage hypoxia exposure, with recovery observed even after 12 h reoxygenation. Differential gene expression highlighted progressive alterations genes associated response, neuroactive ligand interactions, cellular repair mechanisms. Time-series differentially expressed (DEGs) identified critical profiles throughout hypoxia-recovery process hub for each stage. Furthermore, dynamic changes miRNA proteomic indicated active regulation several key biological pathways, including MAPK, HIF-1, ECM-receptor interactions. Through miRNA-mRNA-protein correlation analysis, propose that predicts regulatory pathways interactions across various stages brain E. tetradactylum. study presents first integrated miRNA, mRNA, protein entire brains. The molecular could serve biomarkers future mechanisms fish.
Language: Английский