A microfluidic investigation unveils the role of gravity and shear stress on Pseudomonas fluorescens motility and biofilm growth DOI Creative Commons
Daniele Marra, Manuela Rizzo, Sergio Caserta

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

Abstract Biofilms proliferation in confined environments poses a recurring challenge across several fields, ranging from contamination biomedical devices to fouling of industrial equipment, potential system loss function long term human space missions. It's crucial note that surfaces contact with the fluid, where submerged biofilm develops, experience different bulk stresses resulting combination flow and gravity, factor often overlooked studies. In our research, we aim quantify synergistic effect gravity shear stress on monotrichous bacteria motility growth, considering Pseudomonas fluorescens SBW25 as model organisms. Role was investigated by comparing top bottom rectangular microfluidic channels under controlled laminar flow. Results proved induces asymmetric distribution bacterial cells along channel cell density surface contamination. We report for first time also evolution over during spatial reorganization, providing detailed quantitative analysis classification Both morphology development are affected external mechanical stresses, biocontamination flow, depending intensity direction vector.

Language: Английский

Stress Response of Aspergillus niger Spores to Copper Surfaces and the Implications for Antifungal Surface Functionalization DOI Creative Commons

Stella Marie Timofeev,

Katharina Siems, Daniel Müller

et al.

Advanced Materials Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Abstract Fungal contaminations pose a persistent challenge in the fields of healthcare, agriculture, and industry, primarily due to their environmental adaptability increasing resistance antifungal agents. In this study Aspergillus niger is utilized as model organism. This work evaluates copper, brass, steel surfaces functionalized with ultrashort pulsed laser‐induced periodic surface structures (USP‐DLIP) designed 3 9 µm topographies. spore viability assays show that periodicities on copper achieve 99% reduction viability, indicating increased ion release key factor enhanced effectivity. Scanning electron microscopy (SEM) analysis confirm substantial damage, linked testing measured by inductively coupled plasma triple quadrupole mass spectrometry (ICP‐QQQ) spectrometry. Interestingly, structured reveal trend toward activity despite inert nature. Whereas brass do not significant improvement activity. These findings suggest USP‐DLIP structuring stainless‐steel have considerable potential for applications, although interactions between structures, released ions, fungal spores are highly complex. Yet, offers promising advantages developing advanced materials.

Language: Английский

Citations

0
Microbial contamination and pharmaceutical stability in space environment: addressing dual challenge with innovative technologies and sustainable practices DOI Creative Commons

Manali Patel,

Priya Patel, Nasreen S. Munshi

et al.

Frontiers in Space Technologies, Journal Year: 2025, Volume and Issue: 6

Published: April 30, 2025

As human physiology gets altered in the presence of space flight conditions such as microgravity and radiation, microbes also behave differently space. Many microorganisms have been isolated from stations spacecrafts after travel. Biofilm formation biofilm-related fouling specifically water recovery system are known. Microbes evolved various physiological genetic adaptations which allow them to grow develop biofilm harsh environments well. They contaminate spacecraft environment, its surfaces, system, food products affect astronauts’ health due decreased immunity antibiotic resistance hence, choice antibiotics is a challenging task for treating infection during There negative impact environment on stability antimicrobial agents many get degraded ionizing radiation. This article describes strategies that may decrease risk microbial contamination associated problems expeditions. Various preventive measures suggested use disinfectants periodic cleaning, development coated surfaces kill microbes, space-hardy anti-microbial drugs, etc. Multifaceted approach integrates technological innovation, interdisciplinary collaboration, robust policy frameworks essential. Space based research can further help innovations healthcare management earth.

Language: Английский

Citations

0
Microbial Resilience in Space: Biofilms, Risks and Strategies for Space Exploration DOI
Vinothkannan Ravichandran, Balasubramaniam Krishnan,

Munira Tinwala

et al.

Life Sciences in Space Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
A microfluidic investigation unveils the role of gravity and shear stress on Pseudomonas fluorescens motility and biofilm growth DOI Creative Commons
Daniele Marra, Manuela Rizzo, Sergio Caserta

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

Abstract Biofilms proliferation in confined environments poses a recurring challenge across several fields, ranging from contamination biomedical devices to fouling of industrial equipment, potential system loss function long term human space missions. It's crucial note that surfaces contact with the fluid, where submerged biofilm develops, experience different bulk stresses resulting combination flow and gravity, factor often overlooked studies. In our research, we aim quantify synergistic effect gravity shear stress on monotrichous bacteria motility growth, considering Pseudomonas fluorescens SBW25 as model organisms. Role was investigated by comparing top bottom rectangular microfluidic channels under controlled laminar flow. Results proved induces asymmetric distribution bacterial cells along channel cell density surface contamination. We report for first time also evolution over during spatial reorganization, providing detailed quantitative analysis classification Both morphology development are affected external mechanical stresses, biocontamination flow, depending intensity direction vector.

Language: Английский

Citations

0