Croton hirtus attenuating streptozotocin-induced neuroinflammation in rats DOI Creative Commons

R Prakash,

Jayaram Rajangam,

Binoy Varghese Cheriyan

et al.

Digital Chinese Medicine, Journal Year: 2023, Volume and Issue: 6(3), P. 295 - 306

Published: Sept. 1, 2023

The present study was aimed to investigate the neuroprotective effect of Croton hirtus (CH) extract against streptozotocin (STZ) in rats. (i) sub-chronic toxicity consisted 24 adult rats either sex weighing from 160 200 g were divided into four groups with six each group. Rats group 1 received Dimethyl sulfoxide (DMSO) mixed saline; 2, 3, and 4 100, 200, 400 mg/kg methanolic CH (MECH) orally by gavage administration for 28 d, respectively. functional observation battery locomotor activity graded as part their neurobehavioral brain regions, including cortex hippocampus, analyzed neuropathological abnormalities. (ii) main research 30 male Wistar g, which five group, control (I), STZ (II), Donepezil (III), MECH (100 mg/kg, IV), (200 V) groups. I citrate buffer DMSO saline 14 d. II via intracerebroventricular injection (3 bilateral ICV-STZ) on days 3 followed III, IV, V [3 mg/(kg·d), p.o.] [100 tested learning memory parameters such Morris water maze (MWM) passive avoidance test (PAT). They sacrificed after completing behavioural experiments; brains harvested estimate acetylcholinesterase (AChE), lipid peroxidation (LPO), glutathione (GSH), superoxide dismutase (SOD) using UV-Visible Spectrophotometer; caspase-3 evaluated total fluorescence emission spectra; amyloid β (Aβ) levels detected enzyme-linked immuosorbent assay (ELISA); histopathological examination conducted CA1 region hippocampus. results revealed that open field line crossing, rearing, entering middle square, defecating, or urinating did not differ significantly (P > 0.05). show any lesions neuronal cells inflammation both regions compared findings demonstrated STZ-treated showed a significant increase impairment < 0.001), AChE, caspase-3, Aβ (1-40 1-42), LPO increased decrease found SOD 0.001) GSH 0.01). Moreover, damage In contrast, STZ-induced biochemical impairments considerably decreased treatment dose-dependently. prevented deficit induced due antioxidant action. Restoration cholinergic functioning may be cause improvement. Therefore, able treat cognitive disorders like Alzheimer's disease (AD).

Language: Английский

Natural dietary ROS scavenger-based nanomaterials for ROS-related chronic disease prevention and treatment DOI

Jiani Xie,

Rutuan Dong,

Tairan Zhang

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 490, P. 151756 - 151756

Published: April 30, 2024

Language: Английский

Citations

17
A novel approach to alleviate acetaminophen-induced hepatotoxicity with hybrid balloon flower root-derived exosome-like nanoparticles (BDEs) with silymarin via inhibition of hepatocyte MAPK pathway and apoptosis DOI Creative Commons
Jisu Kim, Chao Gao, Pengcheng Guo

et al.

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: June 18, 2024

Abstract Introduction Balloon flower root-derived exosome-like nanoparticles (BDEs) have recently been proposed as physiologically active molecules with no cytotoxicity. However, the therapeutic effects of drug-induced hepatotoxicity BDEs not elucidated. contain a large amount platycodin D, which is widely known to be effective in regulating inflammation and ameliorating systemic toxicity. Thus, main activity attributed inhibiting inflammatory response alleviating In this study, we fabricated hybrid fused liposomes containing silymarin (SM) enhance synergistic effect on inhibition acetaminophen-induced (APAP). Objective Considering potential BDEs, achieve improve outcomes, constructed soy lecithin-based liposome loaded SM. Since can provide higher thermal stability greater structural integrity, these might more resistant clearance enzymatic degradation drug molecules. Methods Hybrid liposome-loaded SM (BDEs@lipo-SM) were by thin-film hydration extrusion. BDEs@lipo-SM characterized using dynamic light scattering high-performance liquid chromatography. After confirmation physical properties BDEs@lipo-SM, various evaluated. Results internalized hepatocytes immune cells significantly decreased mRNA expression apoptosis inflammation-relevant cytokines hepatocyte MAPK pathway. induced an increase glutathione levels inhibited APAP-induced hepatotoxicity. Conclusion From know that are reliable safe nanovesicles natural metabolites derived from balloon flower, they facilitate intercellular communication. also easily modified loading capacity, targeting effects, long-term accumulation vivo. benefits for acute liver injury alleviate cell death They efficiently delivered effectively inhibit signaling pathway apoptosis, accelerates recovery model. These findings highlight represent attractive delivery vehicle delivery. Graphical abstract

Language: Английский

Citations

5
Dynamics of thioredoxin 1, thioredoxin reductase 1 and glutathione-S-transferase Pi content in the spleen and liver of rats during the latent period of growth and metastasis of sarcoma C45 DOI Creative Commons
Е. И. Сурикова, E. M. Frantsiyants, И. В. Каплиева

et al.

Research and Practical Medicine Journal, Journal Year: 2025, Volume and Issue: 12(1), P. 40 - 51

Published: March 1, 2025

Purpose of the study . To examine dynamics content thioredoxin 1 (Trx1), reductase (TR1), and glutathione S-transferase Pi (GST Pi) in spleen liver during latent period growth metastasis C45, which occurs approximately one to two weeks after tumor transplantation. Materials methods The experiment was conducted on 28 male rats a white, nonlinear strain. A model hematogenous created by transplanting sarcoma 45 (C45) into spleen, previously dislocated under skin three prior commencement experiment. experimental Groups were as follows: Group ( n = 7) comprised intact rats; 2 skin; 3 week later; 4 later. In each case, had undergone transplantation C45 been skin. concentrations Trx1, TR1, GST quantified homogenates through use enzyme linked immunosorbent assay (ELISA). Results. exhibited significantly elevated p ≤ 0.01) Trx1 TR1 level liver, at 8.3 3.4 times higher, respectively, comparison spleen. Conversely, did not demonstrate notable discrepancy between organs. levels diminished relative 1. 3, increased, while 4, these 1.7 (p 0.05) 5.5 0.001) times, compared Additionally, lower average 2. results demonstrated statistically significant increase with threefold found be 4.6 1.8 remained (2.9 0.01), restored observed 1, along Pi. Conclusion initially greater significance Trx system for homeostasis than that is obvious. During metastasis, antioxidant redox regulating functions activated it inhibited, disrupting organ. activity detoxification glutathionylation processes regulated decreased corresponded reflect possible mechanism an imbalance protective metabolic systems forming basis it.

Language: Английский

Citations

0
Ethanolic extract of ginger enhances liver health and lipid metabolism in high-cystine diet-induced hyperlipidemia by boosting the antioxidant milieu DOI Creative Commons

Mohammed A Abdel-Rasol,

Nadia M. El‐Beih,

Wael M. El‐Sayed

et al.

Mediterranean Journal of Nutrition and Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 16, 2025

Background Hypercholesterolemia is a global health concern and risk factor for metabolic disorders due to its association with oxidative stress. Objective This study aimed evaluate the therapeutic potential of ginger ethanolic extract (GE) water suspension (GS) against hypercholesterolemia-induced stress in cystine-fed rats. Methods Rats were randomly assigned seven groups. Control, dimethylsulfoxide (DMSO) group, groups three four received orally 150 mg/kg GE or GS two weeks, respectively. Group five was fed diet 5% cystine weeks. Groups six followed by weeks treatment, Results exhibited significant increases serum alanine aminotransferase alkaline phosphatase activities, hepatic malondialdehyde, levels total cholesterol, triacylglycerols, low density lipoprotein lipids. They showed decreases high cholesterol levels, alpha-1 -2 globulin, beta-globulin reduced glutathione content, activities peroxidase, superoxide dismutase, catalase, reductase, S-transferase. Administration significantly mitigated these effects. Conclusions attenuated damage modulating lipid profile boosting activity antioxidant enzymes showing superior efficacy GS.

Language: Английский

Citations

0
Clostridioides difficile infection induces a pro-inflammatory and pro-steatotic metabolic state in liver DOI Creative Commons

Farnaz Yousefi,

Brett Sponseller,

Muhammed Shafeekh Muyyarikkandy

et al.

npj gut and liver., Journal Year: 2025, Volume and Issue: 2(1)

Published: March 4, 2025

Language: Английский

Citations

0
A Literature Review of Glutathione Therapy in Ameliorating Hepatic Dysfunction in Non-Alcoholic Fatty Liver Disease DOI Creative Commons

M. Hong Nguyen,

Andrew Lian,

Frederick T. Guilford

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(3), P. 644 - 644

Published: March 6, 2025

Non-alcoholic fatty liver disease (NAFLD) is a global cause of dysfunction. This spectrum hepatic disorders can progress to severe conditions, such as non-alcoholic steatohepatitis (NASH) and cirrhosis, due oxidative stress sustained cellular injury. With limited pharmacological options, glutathione (GSH), key antioxidant, has shown promising potential in reducing stress, maintaining redox balance, improving function. literature review examines studies from 2014–2024 exploring GSH therapy NAFLD patients. Eligible assessed the primary intervention for human subjects, reporting outcomes function or markers. Randomized clinical trials (RCTs) were eligible, while combination included if GSH’s effect could be isolated. Exclusions applied non-NAFLD studies, animal/in vitro models, non-GSH antioxidant interventions. Analysis three (totaling 109 participants) demonstrated consistent improvements alanine transaminase (ALT) levels reductions markers like 8-hydroxy-2-deoxyguanosine (8-OHdG). However, small sample sizes inconsistent protocols limit generalizability. Further large-scale RCTs are required confirm efficacy, determine optimal dosing, assess long-term effects. highlights novel therapeutic strategy emphasizing need further refine its application.

Language: Английский

Citations

0
Akebia trifoliata extracts attenuate liver injury via gut–liver axis in a murine model of nonalcoholic fatty liver disease with low-grade colitis DOI
Xiaoya Wang, Han Y. H. Chen, Weifeng Zhu

et al.

Food Research International, Journal Year: 2025, Volume and Issue: unknown, P. 116202 - 116202

Published: March 1, 2025

Language: Английский

Citations

0
Differential effects of ellagic acid on non-alcoholic fatty liver disease in mice: grouped by urolithin A-producing capacity DOI

F Q Li,

Jinxin Luo,

Qi Xie

et al.

Food & Function, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Ellagic acid (EA) exhibits protective effects on non-alcoholic fatty liver disease (NAFLD). However, the ability to produce urolithins and health benefits associated with EA consumption differ considerably among individuals. Therefore, different of high-fat high-fructose diet (HFFD)-induced NAFLD, considering variability in urolithin-producing ability, were explored. Our results showed that could effectively reduce body weight, lipid accumulation insulin resistance, improve oxidative stress inflammation NAFLD mice. The metabolomics analysis indicated metabolism disorder induced by HFFD was obviously improved mainly through regulation unsaturated biosynthesis amino metabolism. In particular, improvement effect mice high urolithin A production better than their low counterparts. Moreover, treatment reshaped gut microbiota imbalance caused HFFD. Specifically, compared model group, lower abundances Faecalibaculum (by 95.11%), Ruminococcus_torques_group 208.14%), Clostridium_sensu_stricto_1 449.37%), Ileibacterium 172.64%), while higher Verrucomicrobia Akkermansia 425.0%) observed high-UroA-producing group (p < 0.05). This study provided new insights into EA's anti-NAFLD effectiveness suggested response capacity greatly determined performance alleviating development NAFLD.

Language: Английский

Citations

0
Therapeutic Potential of Polyphenolic enriched extract of Aesculus indica for Attenuation of Liver Fibrosis through Pectin/Acrylic Acid Based Slow-Release Formulation DOI
Mehwish Khalid, Muhammad Shareef Masoud, Muhammad Tariq

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106938 - 106938

Published: April 1, 2025

Language: Английский

Citations

0
Comprehensive Transcriptome Profiling of Antioxidant Activities by Glutathione in Human HepG2 Cells DOI Creative Commons

Yoshiaki Uchida,

Farhana Ferdousi, Shinya Takahashi

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(5), P. 1090 - 1090

Published: Feb. 29, 2024

Glutathione (GSH) has long been recognised for its antioxidant and detoxifying effects on the liver. The hepatoprotective effect of GSH involves activation antioxidative systems such as NRF2; however, details mechanisms remain limited. A comparative analysis biological events regulated by under physiological oxidative stress conditions also not reported. In this study, DNA microarray was performed with four experiment arms including Control, GSH, hydrogen peroxide (HP), + HP treatment groups. GSH-treated group exhibited a significant upregulation genes clustered in cell proliferation, growth, differentiation, particularly those related to MAPK, when compared Control group. Additionally, liver functions alcohol cholesterol metabolic processes were significantly upregulated. On other hand, HP-induced condition, (GSH group) demonstrated cycle, various signalling pathways (including TGFβ, PI3K/AKT, HIF-1) comparison Furthermore, several disease-related pathways, chemical carcinogenesis–reactive oxygen species fibrosis, downregulated Collectively, our study provides comprehensive both conditions. Our essential insights direct utilisation supplement management associated stress.

Language: Английский

Citations

3