Identification of Anoikis‐Related Genes in Gastric Cancer: Bioinformatics and Experimental Validation

Cancer Medicine, Journal Year: 2025, Volume and Issue: 14(8)

Published: April 1, 2025

ABSTRACT Introduction Distant metastasis is the main reason for poor prognosis of gastric cancer, and anoikis refers to cell death caused when cells detach from extracellular matrix or adhere in incorrect locations, playing an important role distant cancer. Methods Download TCGA‐STAD dataset gene set, filter out differentially expressed genes. Perform consensus clustering cancer samples, conduct Weighted Gene Correlation Network Analysis (WGCNA), enrichment analysis, immune infiltration analysis expression characteristics each subtype, while also filtering genes with differential between subtypes. Additionally, through COX survival identify related establish a nomogram. Finally, validate CYP1B1 vivo vitro clinical culture, establishment model. Results Three subtypes were identified, exhibiting different characteristics, biological pathways, infiltration. The abundance activated NK cells, memory B M2 macrophages showed significant differences among three We screened four sets five (CYP1B1, EQTN, NRXN2, TBC1D3E, TCEAL5) Through we identified 33 independent prognostic constructed nomogram, calibration curves indicating good consistency. selected experimental validation, experiments demonstrated that highly participates resistance promotes invasion, migration, tumor progression cells. Conclusion patterns based on vary, providing theoretical support future personalized treatment

Language: Английский

A novel anoikis-related gene signature can predict the prognosis of hepatocarcinoma patients

Translational Cancer Research, Journal Year: 2024, Volume and Issue: 13(4), P. 1834 - 1847

Published: April 1, 2024

Hepatocellular carcinoma (HCC) is a major health problem with more than 850,000 cases per year worldwide. This cancer now the third leading cause of cancer-related deaths worldwide, and number rising. Cancer cells develop anoikis resistance which vital step during progression metastatic colonization. However, there not much research that specifically addresses role in HCC, especially terms prognosis.

Language: Английский

Anoikis-Related Genes Impact Prognosis and Tumor Microenvironment in Bladder Cancer

Molecular Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 22, 2024

Language: Английский