Laminarin Alleviates Acute Lung Injury Induced by LPS Through Inhibition of M1 Macrophage Polarisation

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(5)

Published: March 1, 2025

The lipopolysaccharide-induced acute lung injury (ALI) mouse model is used to simulate human respiratory distress syndrome (ARDS), which has a high mortality rate. An imbalance between M1 and M2 macrophages, characterised by an increase in was observed sepsis-induced ALI. We report that laminarin, active ingredient found algae, exhibits exceptional performance of It ameliorates edema, enhances the survival rate mice reduces levels inflammatory factors TNF-α IL-6. Furthermore, laminarin reduced expression CD86, are markers associated with macrophages. Laminarin treatment secretion IL-6 LPS-stimulated also decreases glucose uptake Transcriptome sequencing reveals genes downregulated macrophages following predominantly enriched HIF-1α signalling pathway. Experimental validation confirms significantly related indicators ROS NLRP3. After using siRNA knock down RAW264.7 cells, inhibitory effect on LPS-induced polarisation abolished. This suggests may potentially inhibit macrophage towards phenotype downregulating signal. In conclusion, data presented our study demonstrate can effectively reduce signalling. makes it novel candidate drug for

Language: Английский

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HNF4A mitigates sepsis-associated lung injury by upregulating NCOR2/GR/STAB1 axis and promoting macrophage polarization towards M2 phenotype

Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 20, 2025

Sepsis can trigger systemic inflammation and lead to detrimental effects on several organs, with particular emphasis the lungs. In sepsis-associated lung injury, macrophages assume a pivotal role, as their overactivation could facilitate secretion of inflammatory factors imbalance polarization. Hepatocyte nuclear factor 4 alpha (HNF4A) has been reported its potential involvement in regulation response macrophage This study discusses role mechanism HNF4A sepsis-induced damage. exhibits decrease expression by analyzing differentially expressed genes lungs septic mice from Gene Expression Omnibus dataset GSE15379. Then, we established mouse sepsis model through cecal ligation puncture method observed that was reduced both tissues alveolar macrophages. To evaluate function HNF4A, overexpressed mediated adenovirus vectors, which were injected into mice. We found overexpression resulted higher survival rate an amelioration pulmonary Meanwhile, mitigated infiltration cells impeded M1 polarization but facilitated M2 or lavage fluid. vitro, treated bone marrow-derived interleukin-4. Consistent results obtained promoted Mechanistically, transcriptionally regulate receptor coactivator 2 (NCOA2) binding promoter region. NCOA2 interacted glucocorticoid (GR). Stabilin 1 (STAB1) selected possible target transcriptome sequencing analysis. Functional experiments confirmed STAB1 downstream HNF4A/NCOA2/GR axis. Overall, this research investigated impact injury sepsis. It is suggested one regulatory mechanisms involved association may be NCOR2/GR/STAB1

Language: Английский

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Chest radiographs in acute respiratory distress syndrome: an Achilles' heel of the Berlin criteria?

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12

Published: April 17, 2025

Despite the high mortality and economic burden associated with acute respiratory distress syndrome (ARDS), role of chest radiograph (CXR) in ARDS diagnosis prognosis remains uncertain. The purpose this study is to elucidate clinical characteristics that distinguish patients from those without ARDS, especially where CXRs are indicative ARDS. Secondary analysis a prospective observational 454 postoperative septic under mechanical ventilation (MV). Patients were stratified two groups depending on whether they met Berlin criteria for Primary outcome was identification differentiating confirmed by CXR non-ARDS patients. 60-day in-hospital sepsis-induced One hundred thirty-nine (30.6%) had compatible although only 45 (9.9%). Emergency surgery (OR 6.6), abdominal source infection 6.0), pneumonia 8.2), higher lactate 3.9) features development CXR. an independent risk factor 1.8). Although could be replaced future definitions, its importance should not underestimated.

Language: Английский

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Single-center nomogram model for sepsis complicated by acute lung injury

American Journal of Translational Research, Journal Year: 2024, Volume and Issue: 16(9), P. 4653 - 4661

Published: Jan. 1, 2024

To construct and validate a nomogram model for predicting sepsis complicated by acute lung injury (ALI).

Language: Английский

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