Exploring the Therapeutic Potential of MIR‐140‐3p in Osteoarthritis: Targeting CILP and Ferroptosis for Novel Treatment Strategies

Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

Osteoarthritis (OA) is a prevalent and debilitating joint disorder that affects millions of individuals worldwide, severely impairing mobility, independence, quality life. Emerging evidence suggests ferroptosis critical factor in OA pathogenesis. However, its precise involvement underlying mechanisms remain poorly understood. In this study, we first identified cartilage intermediate layer protein (CILP) mediates the regulation ferroptosis-related genes through hdWGCNA analysis combined with single-cell RNA sequencing. Further investigation revealed significant upregulation CILP expression C28/I2 cells under LPS induction. Mechanistically, bioinformatics differentially expressed miRNAs; qRT-PCR dual-luciferase experiment miR-140-3p was downregulated directly targets CILP. Experimental data further demonstrated regulates ferroptosis, inflammation, oxidative stress by targeting These findings offer valuable insights into molecular miR-140-3p/CILP axis regulating stress, thus providing foundation for developing therapeutic strategies OA.

Language: Английский

Ferroptosis in immune chaos: Unraveling its impact on disease and therapeutic potential

Journal of Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Language: Английский