Efficacy and Mechanism of Thymol/KGM/LG Edible Coating Solution on Inhibition of Mucor circinelloides Isolated From Okra DOI Creative Commons
Qinqiu Zhang, Wen Qin,

Xinjie Hu

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: May 16, 2022

With the increasing demand and quality requirement for natural nutritious food in modern society, okra has attracted much attention because of its high nutritional value remarkable functionality. However, occurrence postharvest diseases fresh severely limited application okra. Therefore, this study, dominant pathogens causing such as soft rot were isolated from naturally decaying It was identified Mucor circinelloides by morphological characteristics standard internal transcribed spacer ribosomal DNA sequence. Furthermore, biological M. studied, inhibitory effect thymol/KGM/LG (TKL) edible coating solution on possible mechanism discussed. In addition, TKL had a dose-dependent circinelloides, with 50% concentration (EC50) 113.55 mg/L. The at 960 mg/L thymol completely inhibited mycelial growth spore germination circinelloides. results showed that best carbon source maltose, nitrogen beef extract potassium nitrate, pH 6, temperature 28°C, NaCl 0.5%, light conducive to also observed scanning electron microscope (SEM) more likely destroy cell wall integrity inhibit morphology change mycelium structure. Meanwhile, activity chitinase (CHI), an enzyme related synthesis significantly decreased after being treated 100 (TKL100). content Malondialdehyde (MDA) 12 h 48 h, which may cause oxidative damage membrane. polygalacturonase (PG), pectin methylgalacturonase (PMG), cellulase (Cx) significantly. good bacteriostatic pathogen, main might be membrane, degradation wall, inhibition metabolic activities, reduction metabolites, is helpful further understand pathogen mechanism.

Language: Английский

Antarctic Streptomyces: Promising biocontrol agents for combating Fusarium oxysporum f. sp. cubense DOI Creative Commons
Jeffrey Vargas Pérez, Lizette Serrano, Rafael Viteri

et al.

Biotechnology Reports, Journal Year: 2024, Volume and Issue: 43, P. e00852 - e00852

Published: Aug. 23, 2024

Fusarium wilt of Banana (FWB) caused by oxysporum f. sp. cubense (Foc) poses a significant threat to the banana industry, with current inadequate control measures. This study evaluated antifungal potential nine Streptomyces strains isolated from Antarctic soil samples, using Casein-Starch media stimulate production compounds. The inhibition spectrum against Foc was assessed under laboratory conditions well diffusion on Mueller-Hinton agar, activity measured in arbitrary units (AU/mL) and minimum inhibitory concentration (MIC) tested ethyl acetate extracts. Among isolates, K6 E7 were closely related polyrhachis fildesensis, exhibited activity, showing 320 80 AU/mL, MIC values 250 >500 ppm, respectively. These findings highlight as biocontrol agents Foc, offering new avenues for sustainable management cultivation.

Language: Английский

Citations

2
Critical Secondary Metabolites Confer the Broad-Spectrum Pathogenic Fungi Resistance Property of a Marine-Originating Streptomyces sp. HNBCa1 DOI
Zi Xiong, Tengfei Xia, Weicheng Wu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(23), P. 13164 - 13174

Published: May 31, 2024

Obtaining a microorganism strain with broad-spectrum resistance property and highly efficient antifungal activity is important to the biocontrol strategy. Herein, marine

Language: Английский

Citations

1
A smooth vetch (Vicia villosa var.) strain endogenous to the broad-spectrum antagonist Bacillus siamensis JSZ06 alleviates banana wilt disease DOI Creative Commons

Yan-Nan Ruan,

Caihong Nong,

Attachai Jintrawet

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: June 4, 2024

wilt, caused by

Language: Английский

Citations

1
Identification, fermentation optimization, and biocontrol efficacy of actinomycete YG-5 for the prevention of Alternaria leaf spot disease in star anise DOI Creative Commons

Jieming Pan,

Xiaoshan Geng,

Yujing Cai

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 10, 2024

Star anise (Illicium verum), a valuable spice tree, faces significant threats from fungal diseases, particularly Alternaria leaf spot. This study investigates the potential of soil-derived actinomycete strain, YG-5, as biocontrol agent against tenuissima, causative pathogen on spot in star anise. Through comprehensive morphology, physiology, biochemistry, and genetic analyses, we identified isolate Streptomyces sp. YG-5. The strain exhibited broad-spectrum antimicrobial activity several plant pathogens, with inhibition rates ranging between 36.47 to 80.34%. We systematically optimized fermentation conditions for including medium composition cultivation parameters. process resulted an 89.56% rate A. 14.72% improvement over non-optimized conditions. Notably, compounds produced by YG-5 demonstrated stability across various temperatures, pH levels, UV irradiation. In vivo efficacy trials showed promising results, broth reducing incidence leaves 56.95%. These findings suggest that holds cultivation, offering sustainable approach disease management this crop.

Language: Английский

Citations

1
Efficacy and Mechanism of Thymol/KGM/LG Edible Coating Solution on Inhibition of Mucor circinelloides Isolated From Okra DOI Creative Commons
Qinqiu Zhang, Wen Qin,

Xinjie Hu

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: May 16, 2022

With the increasing demand and quality requirement for natural nutritious food in modern society, okra has attracted much attention because of its high nutritional value remarkable functionality. However, occurrence postharvest diseases fresh severely limited application okra. Therefore, this study, dominant pathogens causing such as soft rot were isolated from naturally decaying It was identified Mucor circinelloides by morphological characteristics standard internal transcribed spacer ribosomal DNA sequence. Furthermore, biological M. studied, inhibitory effect thymol/KGM/LG (TKL) edible coating solution on possible mechanism discussed. In addition, TKL had a dose-dependent circinelloides, with 50% concentration (EC50) 113.55 mg/L. The at 960 mg/L thymol completely inhibited mycelial growth spore germination circinelloides. results showed that best carbon source maltose, nitrogen beef extract potassium nitrate, pH 6, temperature 28°C, NaCl 0.5%, light conducive to also observed scanning electron microscope (SEM) more likely destroy cell wall integrity inhibit morphology change mycelium structure. Meanwhile, activity chitinase (CHI), an enzyme related synthesis significantly decreased after being treated 100 (TKL100). content Malondialdehyde (MDA) 12 h 48 h, which may cause oxidative damage membrane. polygalacturonase (PG), pectin methylgalacturonase (PMG), cellulase (Cx) significantly. good bacteriostatic pathogen, main might be membrane, degradation wall, inhibition metabolic activities, reduction metabolites, is helpful further understand pathogen mechanism.

Language: Английский

Citations

3