An Immunoinformatic‐Based In Silico Identification on the Creation of a Multiepitope‐Based Vaccination Against the Nipah Virus

BioMed Research International, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

The zoonotic viruses pose significant threats to public health. Nipah virus (NiV) is an emerging transmitted from bats humans. NiV causes severe encephalitis and acute respiratory distress syndrome, leading high mortality rates, with fatality rates ranging 40% 75%. first emergence of the disease was found in Malaysia 1998–1999 later Bangladesh, Cambodia, Timor‐Leste, Indonesia, Singapore, Papua New Guinea, Vietnam, Thailand, India, other South Southeast Asian nations. Currently, no specific vaccines or antiviral drugs are available. potential advantages epitope‐based include their ability elicit immune responses while minimizing side effects. epitopes have been identified conserved region viral proteins obtained UniProt database. selection involves analyzing genetic sequences various strains. present study two B cell epitopes, seven cytotoxic T lymphocyte (CTL) helper (HTL) epitope interactions proteomic inventory. antigenic physiological properties retrieved protein were analyzed using online servers ToxinPred, VaxiJen v2.0, AllerTOP. final vaccine candidate has a total combined coverage range 80.53%. tertiary structure constructed optimized, its stability confirmed help molecular simulation. Molecular docking performed check binding affinity energy TLR‐3 TLR‐5. Codon optimization within Escherichia coli K12 strain, eliminate danger codon bias. However, these findings must require further validation assess effectiveness safety. development therapeutic approaches for infection ongoing area research, it may take time before effective interventions available clinical use.

Language: Английский

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An attachment glycoprotein nanoparticle elicits broadly neutralizing antibodies and protects against lethal Nipah virus infection

npj Vaccines, Journal Year: 2024, Volume and Issue: 9(1)

Published: Aug. 31, 2024

Nipah virus (NiV) is a zoonotic emergent paramyxovirus that can cause severe encephalitis and respiratory infections in humans, with high fatality rate ranging from 40% to 75%. Currently, there are no approved human vaccines or antiviral drugs against NiV. Here, we designed ferritin-based self-assembling nanoparticle displaying the NiV G head domain on surface (NiV G-ferritin) assessed immune responses elicited by soluble sG) G-ferritin. Immunization G-ferritin sG conferred complete protection lethal challenge without detection of viral RNA Syrian golden hamsters. Compared sG, induced significantly faster, broader, higher serum neutralizing three pathogenic henipaviruses (NiV-Malaysia, NiV-Bangladesh, Hendra virus). Moreover, durable immunity mice as antisera potently inhibited infection even after six months third immunization. Additionally, isolated panel 27 G-binding monoclonal antibodies (mAbs) immunized found these mAbs targeted four distinct antigenic sites two have not been defined previously. Notably, 25 potent activity 50% inhibitory concentrations less than 10 ng/mL pseudovirus. Collectively, findings provide new insights into immunogenicity protein reveal safe highly effective vaccine candidate infection.

Language: Английский

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Risk of Viral Infectious Diseases from Live Bats, Primates, Rodents and Carnivores for Sale in Indonesian Wildlife Markets

Viruses, Journal Year: 2022, Volume and Issue: 14(12), P. 2756 - 2756

Published: Dec. 10, 2022

Southeast Asia is considered a global hotspot of emerging zoonotic diseases. There, wildlife commonly traded under poor sanitary conditions in open markets; these markets have been ‘the perfect storm’ for disease transmission. We assessed the potential trade spreading viral diseases by quantifying number wild animals four mammalian orders (Rodentia, Chiroptera, Carnivora and Primates) on sale 14 Indonesian identifying viruses potentially hosted animals. constructed network analysis to visualize that are alongside each other may carry similar viruses. recorded 6725 at least 15 species sale. Cities with larger human population stalls, respectively, offered more individuals Eight out animal taxa hosts 17 virus species, nine which can infect than one as host. The showed long-tailed macaque has greatest diseases, since it simultaneously most sold 13/14 markets, host It pig-tailed macaques three shares six common (Cowpox, Dengue, Hepatitis E, Herpes B, Simian foamy, retrovirus type D). Short-nosed fruit bats large flying foxes Nipah also quantities 10/14 markets. This study highlights need better surveillance avoid negative health impacts unregulated

Language: Английский

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Current status of diagnostic assays for emerging zoonotic viruses: Nipah and Hendra

Expert Review of Molecular Diagnostics, Journal Year: 2024, Volume and Issue: 24(6), P. 473 - 485

Published: June 2, 2024

Nipah and Hendra viruses belong to the Paramyxoviridae family, which pose a significant threat human health, with sporadic outbreaks causing severe morbidity mortality. Early symptoms include fever, cough, sore throat, headache, offer little in terms of differential diagnosis. There are no specific therapeutics vaccines for these viruses.

Language: Английский

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What have we learnt from the COVID-19 pandemic?

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 867 - 892

Published: Jan. 1, 2025

Language: Английский

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Computational Analysis of Nipah Virus Transmission Using Mathematical Modelling and Graphical Programming

BioNanoScience, Journal Year: 2025, Volume and Issue: 15(2)

Published: Feb. 3, 2025

Language: Английский

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Viral infections of pig: Signs, prevention, and control

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 243 - 262

Published: Jan. 1, 2025

Language: Английский

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A Stable and Dependable Visual Technique for On-Site Nipah Virus Nucleic Acids Detection

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 27, 2025

Abstract The Nipah Virus (NiV) is a zoonotic pathogen with the mortality rate of up to 75%, recurring in Asia over past two decades. Due increasing risk human transmission mediated by various intermediate hosts such as pigs and bats, it necessary produce an accurate reliable point-of-care molecular detection method for NiV field diagnosis. In this study, we designed pairs primers targeting conserved G P genes developed nucleic acid (POC-NAD) system integrating one-step RT-PCR, lateral flow immunoassay, microfluidic technologies. POC-NAD shows high specificity sensitivity, Limit Detection (LoD) 199.1 copies/rxn. aiming sequences enables simultaneous both NiV-M NiV-B strains. Continuous evaluation 21 simulated clinical samples demonstrated 100% concordance RT-PCR results. Lateral flow-based visualization improves display time legibility Additionally, chips or chambers offer disposable reagent containers consistent PCR amplification results across conditions. Therefore, diagnostic tool suitable real-time testing surveillance resource-limited environments.

Language: Английский

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Bangladesh <i>Nipah virus</i>: The Emerging Threat of Global Spread

Journal of Health Reports and Technology, Journal Year: 2025, Volume and Issue: 11(2)

Published: Feb. 15, 2025

Language: Английский

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Evolutionary trajectories of Nipah virus: Evaluating the antiviral efficacy of Kabasura Kudineer Chooranam

Computers in Biology and Medicine, Journal Year: 2025, Volume and Issue: 190, P. 109973 - 109973

Published: April 3, 2025

Language: Английский

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