A review of a colorimetric biosensor based on Fe3O4 nanozymes for food safety detection

Analytical and Bioanalytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 13, 2024

Language: Английский

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State of the art CRISPR-based strategies for cancer diagnostics and treatment

Biomarker Research, Journal Year: 2024, Volume and Issue: 12(1)

Published: Dec. 18, 2024

Abstract Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology is a groundbreaking and dynamic molecular tool for DNA RNA “surgery”. CRISPR/Cas9 the most widely applied system in oncology research. It major advancement genome manipulation due to its precision, efficiency, scalability versatility compared previous gene editing methods. has shown great potential not only targeting of oncogenes or genes coding immune checkpoint molecules, engineering T cells, but also epigenomic disturbances, which contribute cancer development progression. proven useful detecting genetic mutations, enabling large-scale screening involved tumor onset, progression drug resistance, speeding up highly targeted therapies tailored immunological profiles patient’s tumor. Furthermore, recently discovered Cas12 Cas13 systems have expanded Cas9-based applications, providing new opportunities diagnosis treatment cancer. In addition traditional cis-cleavage, they exhibit trans-cleavage activity, enables their use as sensitive specific diagnostic tools. Diagnostic platforms like DETECTR, employs enzyme, that cuts single-stranded reporters, SHERLOCK, uses Cas12, Cas13, specifically target cleave RNA, can be exploited speed advance oncological diagnostics. Overall, CRISPR platform improve diagnostics functionality safety engineered cellular medicines. Here, we will emphasize potentially transformative impact field treatments, prognostic approaches, highlight challenges raised by using newly introduced CRISPR-based therapy.

Language: Английский

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CRISPR‑based diagnostic approaches: Implications for rapid management of future pandemics (Review)

Molecular Medicine Reports, Journal Year: 2023, Volume and Issue: 27(6)

Published: May 3, 2023

Sudden viral outbreaks have increased in the early part of 21st century, such as those severe acute respiratory syndrome coronavirus (SARS‑CoV), Middle East corona virus, and SARS‑CoV‑2, owing to human access wildlife habitats. Therefore, likelihood zoonotic transmission human‑associated viruses has increased. The emergence 2 China its spread worldwide within months highlighted need be ready with advanced diagnostic antiviral approaches treat newly emerging diseases minimal harm health. gold‑standard molecular currently used are time‑consuming, require trained personnel sophisticated equipment, therefore cannot point‑of‑care devices for widespread monitoring surveillance. Clustered regularly interspaced short palindromic repeats (CRISPR)‑associated (Cas) systems been reported bacteria, archaea bacteriophages. CRISPR‑Cas organized into CRISPR arrays adjacent Cas proteins. detection in‑depth biochemical characterization class type V VI orthologous proteins Cas12 Cas13 led development CRISPR‑based approaches, which detect distinguish between serotypes subtypes. single nucleotide polymorphisms samples from patients cancer agents destroy that contain RNA a genome. likely improve disease methods century their ease development, low cost, reduced turnaround time, multiplexing deployment. present review discusses properties orthologs other applications. expands scope fight antivirals.

Language: Английский

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Identification of multi-cancer risk genes

Published: Jan. 1, 2024

Cancer is the second main cause of death in world, and genetic variation influences a large proportion its risk. This thesis combined GWAS, 3D chromatin organisation transcriptomic data to identify candidate hormone-related skin cancer risk genes. A total 78 MCR genes were associated with at least two cancers, whilst 148 protein-coding lncRNAs for identified. The intersection between features risk-associated SNPs provide powerful tool identification new

Language: Английский

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Advances in CRISPR/Cas systems-based cell and gene therapy

Progress in molecular biology and translational science, Journal Year: 2024, Volume and Issue: unknown, P. 161 - 183

Published: Jan. 1, 2024

Language: Английский

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