Longitudinal study of the bovine cervico-vaginal bacterial microbiota throughout pregnancy using 16S ribosomal RNA gene sequences DOI Creative Commons
Lucía Calleros,

Maila Barcellos,

Sofía Grecco

et al.

Infection Genetics and Evolution, Journal Year: 2024, Volume and Issue: 124, P. 105657 - 105657

Published: Aug. 19, 2024

The microbiota composition of the bovine female reproductive tract influences efficiency, susceptibility to genital pathogens, and health newborn calves. However, knowledge about cervico-vaginal during gestation is scarce. Therefore, present study aimed analyze taxonomic profile throughout pregnancy after calving using high-throughput sequencing a fragment 16S ribosomal RNA gene. Healthy nulliparous Holstein heifers (n = 13) with similar age body conditional score were selected collect samples from area sterile swab at 5 timepoints. We sequenced V1-V2 region gene analyzed data DADA2, phyloseq vegan R Studio packages. No differences observed in alpha beta diversity across sampling points, accounting for stability pregnancy. most abundant phyla are Firmicutes, Bacteroidota, Proteobacteria Actinobacteria, as main taxa all five points. Also, several least can be change time. Our comprehensive bacterial period contributes dynamics on serve baseline future research development potential therapeutic interventions.

Language: Английский

Whole-body Microbiota of Newborn Calves and Their Response to Prenatal Vitamin and Mineral Supplementation DOI Creative Commons

Sarah M. Luecke,

Devin B. Holman,

Kaycie N. Schmidt

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: March 21, 2023

ABSTRACT Here, we investigated whether initial microbial colonization at seven different anatomical locations in newborn calves and their blood cytokines are influenced by prenatal vitamin mineral (VTM) supplementation. Samples were collected from the hoof, liver, lung, nasal cavity, eye, rumen (tissue fluid), vagina of beef that born dams received diets with or without VTM supplementation throughout gestation (n=7/group). Calves separated immediately after birth fed colostrum milk replacer until euthanasia 30 h post-initial feeding. The microbiota all samples was assessed using 16S rRNA gene sequencing qPCR. 15 chemokines quantified serum. ocular, respiratory, reproductive sites colonized site-specific differed (0.64 ≥ R 2 0.12, P ≤ 0.003). Only ruminal fluid on ( P< 0.01 Differences <0.05) detected richness (vagina), diversity (ruminal tissue fluid, eye), composition phylum genus level vagina), total bacterial abundance (ocular vagina) between control calves. cytokine IP-10 higher =0.02) Overall, our results suggest despite immediate separation dam upon birth, whole-body 32-h old relatively rich, diverse communities, rumen, oculus seem to be IMPORTANCE Increased appreciation maternal nutrition microbiome’s involvement developmental programming evidence supporting utero highlight factors could impact offspring colonization. any 7 We identified changes ruminal, vaginal, ocular response provided a “holistic” view calf microbiota. Our data obtained same sex age, who dams, hence provides novel insights taxonomic those examined. Combined, this study direction for future work targeting manipulation early life microbiome via alteration harnessing improved cattle health production.

Language: Английский

Citations

2
Sequencing and Culture-based Characterization of the Vaginal and Uterine Microbiota in Beef Cattle that Became Pregnant or Non-pregnant via Artificial Insemination DOI Creative Commons

Emily M. Webb,

Devin B. Holman,

Kaycie N. Schmidt

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 31, 2023

Abstract In this study, we evaluated the vaginal and uterine microbiota between beef cattle that became pregnant via artificial insemination (AI) those did not to identify microbial signature associated with pregnancy. We also characterized culturable fraction of these using extensive culturing screened some bacterial isolates for their antimicrobial resistance. For this, swabs from two cohorts Angus-crossbred cattle: mature cows (vaginal uterine; 27 open 31 pregnant) heifers (vaginal; 26 33 were collected before AI processed assessment 16S rRNA gene sequencing culturing. Twenty-nine resistance against 41 antibiotics. Sequencing results revealed 11 taxa more abundant in samples non-pregnant compared heifers. No differentially detected versus cows. Pregnant had a distinct community structure ( P = 0.008) interaction network Twenty-eight observed groups. Community diversity different cow vagina uterus. A total 733 recovered (512) (221) under aerobic (83 species) anaerobic (69 Among pathogenic species mostly susceptible tested Overall, our indicate pregnancy-associated taxonomic signatures are present bovine microbiota. Importance Emerging evidence suggests microbiome-targeted approaches may provide novel opportunity reduce incidence reproductive failures cattle. To develop such microbiome-based strategies, one first logical steps is microbiome features related fertility, isolate pregnancy developing future consortium could be administered breeding enhance outcomes. Here, or identified similarities microbiota, Using culturing, provided new insights on findings will serve as an important basis research aimed at harnessing improved fertility.

Language: Английский

Citations

2
16s gene metagenomic characterization in healthy stallion semen DOI
Kattia Núñez-Montero, Karla Leal, Dorian Rojas-Villalta

et al.

Research in Veterinary Science, Journal Year: 2024, Volume and Issue: 176, P. 105354 - 105354

Published: July 4, 2024

Language: Английский

Citations

0
The microbiome effect on the female reproductive performance DOI Creative Commons
Ricardo Zanella, Janine de Camargo, Cláudia Almeida Scariot

et al.

Animal Reproduction, Journal Year: 2024, Volume and Issue: 21(3)

Published: Jan. 1, 2024

The female reproductive function is coordinated by the endocrine system driven hypothalamic-pituitary-gonadal (HPG) axis. While not directly part of system, gut microbiome plays a crucial role in overall health, including health. communicates bidirectionally with brain via gut-brain axis, influencing stress levels, mood, and hormonal balance, which can impact health fertility. In addition to that, vaginal uterine are involved success farm animals, fertility offspring development. this paper, we summarize some effects bacterial contamination tract their association performance animals.

Language: Английский

Citations

0
Longitudinal study of the bovine cervico-vaginal bacterial microbiota throughout pregnancy using 16S ribosomal RNA gene sequences DOI Creative Commons
Lucía Calleros,

Maila Barcellos,

Sofía Grecco

et al.

Infection Genetics and Evolution, Journal Year: 2024, Volume and Issue: 124, P. 105657 - 105657

Published: Aug. 19, 2024

The microbiota composition of the bovine female reproductive tract influences efficiency, susceptibility to genital pathogens, and health newborn calves. However, knowledge about cervico-vaginal during gestation is scarce. Therefore, present study aimed analyze taxonomic profile throughout pregnancy after calving using high-throughput sequencing a fragment 16S ribosomal RNA gene. Healthy nulliparous Holstein heifers (n = 13) with similar age body conditional score were selected collect samples from area sterile swab at 5 timepoints. We sequenced V1-V2 region gene analyzed data DADA2, phyloseq vegan R Studio packages. No differences observed in alpha beta diversity across sampling points, accounting for stability pregnancy. most abundant phyla are Firmicutes, Bacteroidota, Proteobacteria Actinobacteria, as main taxa all five points. Also, several least can be change time. Our comprehensive bacterial period contributes dynamics on serve baseline future research development potential therapeutic interventions.

Language: Английский

Citations

0