Contrasting response of comammox Nitrospira, ammonia oxidising bacteria, and archaea to soil pH and nitrogen inputs

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 924, P. 171627 - 171627

Published: March 11, 2024

This study aimed to investigate the effect of soil pH change, and nitrogen amendment on ammonia oxidiser abundance comammox Nitrospira community composition. The experimental design used mesocosms placed in a temperature-controlled incubator for 90 days. A Templeton silt loam was as its physiochemical properties are typical region's dairy farms. results showed that clade B preferred natural (pH 6.1–6.2) with no applied nitrogen. Furthermore, synthetic urine (N700) decreased B. may have been because large amounts available N700 treatments inhibited growth Nitrospira. These suggest while present New Zealand farm soils, but their role nitrification very high environment under patch grazed pastures be limited. Further research is needed confirm this. In contrast comammox, AOB (dominated by Nitrosospira) responded positively application urine. response greatest (7.1), followed then low (4.9) soils. due difference availability. At pH, ammonia/ammonium equilibrium favours production. Calculated availability accurately predicted amoA gene abundance. Interestingly, AOA (which predominantly made up Thaumarchaeota group I.1b E) seemed prefer soils over pH. specific lineage present. did not respond

Language: Английский

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Nitrification inhibitor chlorate and nitrogen substrates differentially affect comammox Nitrospira in a grassland soil

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: May 14, 2024

Through the combined use of two nitrification inhibitors, Dicyandiamide (DCD) and chlorate with nitrogen amendment, this study aimed to investigate contribution comammox

Language: Английский

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Visualizing small-scale subsurface NH3 and pH dynamics surrounding nitrogen fertilizer granules and impacts on nitrification activity

Soil Biology and Biochemistry, Journal Year: 2023, Volume and Issue: 189, P. 109273 - 109273

Published: Dec. 8, 2023

In agricultural soils, nitrogen (N) fertilizer is typically applied as ammonium salts or urea, and undergoes microbially mediated oxidation. However, insights regarding substrate concentrations nitrifiers are exposed to in the zones immediately surrounding N granules remain unexamined, well how this affects activity inhibition of nitrifier groups adapted different ranges ammonia (NH3) concentrations. To examine millimeter scale changes soil chemistry after sub-surface granule application with high spatiotemporal resolution, we a newly developed NH3 pH planar optical sensor (optode) system. With system, visualized situ subsurface production, diffusion, associated shifts an following (from 5 min up 65.5 h) chloride (NH4Cl) urea laboratory study. Ammonia from both NH4Cl diffused 1.5 2 cm reached maximum >49 >130 ppmv, respectively. Spatially informed destructive subsampling (0.5–2 granules) revealed that nitrate accumulation was greatest closest (0.5–1 cm) granules, while nitrite not detected. contrast, resulted accumulation, highest detected furthest (1.5–2 granules. Urea hydrolysis also caused significant localized increase, circular elevated zone migrating outwards leaving decreased at center. Transcription ammonia-oxidizing bacterial amoA gene areas there were no differences transcription increasing distance Ammonia-oxidizing archaea comammox transcripts This study first directly visualize small-scale concentration relate these nitrification activity.

Language: Английский

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Manganese-mediated ammonium removal by a bacterial consortium from wastewater: Experimental proof and biochemical mechanisms

Bioresource Technology, Journal Year: 2023, Volume and Issue: 385, P. 129353 - 129353

Published: June 17, 2023

Language: Английский

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Journey of the swift nitrogen transformation: Unveiling comammox from discovery to deep understanding

Chemosphere, Journal Year: 2024, Volume and Issue: 358, P. 142093 - 142093

Published: April 26, 2024

Language: Английский

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Response of comammox Nitrospira clades A and B communities to long-term fertilization and rhizosphere effects and their relative contribution to nitrification in a subtropical paddy field of China

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 367, P. 121939 - 121939

Published: July 27, 2024

Language: Английский

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Nitrification inhibitors impose distinct effects on comammox bacteria and canonical ammonia oxidizers under high N fertilization regimes

Applied Soil Ecology, Journal Year: 2024, Volume and Issue: 199, P. 105417 - 105417

Published: April 20, 2024

The response of complete ammonia-oxidizing (comammox) bacteria to variable nitrogen (N) inputs and nitrification inhibitors (NIs) remains poorly understood. We conducted two experiments, in a soil where both clade A B comammox Nitrospira were present, identify the type (ammonium sulphate vs. urea) level N-fertilization (0–500 mg-N-kg‐1d.w. soil) that are conducive growth comammox, compared canonical microorganisms (AOM), subsequently assess their three commercial (DCD, nitrapyrin, DMPP) new potent (ethoxyquin) NI under these favourable N fertilization regimes. highest concentration ammonium stimulated AOM which showed dose-dependent increase by sources. DMPP primarily, DCD, imposed more persistent inhibition on than nitrapyrin ethoxyquin, result did not concur with persistence NIs (DMPP was least among NIs) but mostly driven selective inhibitory activity (AOB) prevailed studied soil. AOB DCD complemented proliferation archaea (AOA). induced significant changes communities. Comammox enriched depleted ethoxyquin. (i) releasing AOA from competition allows them proliferate efficiently, even high inorganic conditions; (ii) responsive input NIs.

Language: Английский

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Transcriptional activity of ammonia oxidisers in response to soil temperature, moisture and nitrogen amendment

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 15, 2025

The contrasting response of AOA, AOB, and comammox Nitrospira amoA transcript abundance to temperature, moisture, nitrogen was investigated using soil microcosms. treatments were selected represent conditions typically found in a New Zealand (NZ) dairy farm. AOB dominated all synthetic urine treated soils. Peak positively correlated with estimated ammonia availability. While gDNA nitrification rate trends similar. AOA strongly influenced by temperature. At 20°C, peak averaged over 1 order magnitude higher than at 8°C. Within the community member Nitrosocosmicus clade ammonium concentrations. presence relative increase an high environment poses interesting contrast current scientific opinion NZ. Comammox showed no correlation moisture. This suggests that previously associations are more complex originally thought. Further research is required determine drivers moisture environment. Overall, these results indicate main farm

Language: Английский

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Exploring Suitable Nitrification Inhibitor in an Intensively Cultivated Greenhouse Soil and Its Effect on the Abundance and Community of Soil Ammonia Oxidizers

Agronomy, Journal Year: 2025, Volume and Issue: 15(2), P. 255 - 255

Published: Jan. 21, 2025

The application of nitrification inhibitors (NIs) is an effective way to reduce soil nitrogen (N) losses and increase crop N uptake. Yet, the efficacy NIs commonly varies with dosages, systems environmental conditions. Hence, clarifying suitable type dosage extremely important for structuring best management regime at a regional scale. Here, based on microcosm experiments, we evaluated influence three widely used [Dicyandiamide, DCD; 3,4-Dimethylpyrazole phosphate, DMPP; 2-chloro-6-(trichloromethyl) pyridine, Nitrapyrin] activity intensively cultivated greenhouse soil. results showed that both DCD DMPP imposed transient inhibition (less than five days) regardless dosages applied, and, contrary, Nitrapyrin presented persistent suppression, longer duration action by higher dosage. Accordingly, incorporation 2% applied rate (w/w) recommendable local intensive production. Further, assessed various (0%, 0.25%, 0.5%, 5%) abundance community groups ammonia oxidizers [i.e., ammonia-oxidizing archaea (AOA), bacteria (AOB) completely (Comammox Nitrospira)] qPCR high-throughput amplicon sequencing. strongly lowered AOB Comammox Nitrospira abundances their richness even lowest also significantly altered structure all tested oxidizers, average relative some major members (i.e., Nitrososphaerales Clade Nitrososphaera, A, Nitrosospira briensis Clade, multiformis A.2 A-associated) obviously responded incorporation. Overall, our findings indicated were more sensitive as compared AOA. obtained here highlight importance optimizing fertilization in vegetable inhibits probably suppressing level herein.

Language: Английский

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Revisiting the interplay between Comammox and Anammox: cooperative or competitive?

Journal of Water Process Engineering, Journal Year: 2025, Volume and Issue: 75, P. 107918 - 107918

Published: May 10, 2025

Language: Английский

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