Development of adaptive anoikis resistance promotes metastasis that can be overcome by CDK8/19 Mediator kinase inhibition DOI Creative Commons
Mehri Monavarian, Emily Faith Page, Resha Rajkarnikar

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 6, 2023

Anoikis resistance or evasion of cell death triggered by detachment into suspension is a hallmark cancer that concurrent with survival and metastasis. The effects frequent matrix encounters on the development anoikis in remains poorly defined. Here we show using panel ovarian models, repeated exposure to stress vitro followed attached recovery growth leads paralleling vivo ascites. This enhanced invasion, chemoresistance ability adapted cells metastasize distant sites. Adapted resistant heightened dependency oxidative phosphorylation can also evade immune surveillance. We find such acquired not genetic, as persists for finite duration absence stress. Transcriptional reprogramming however essential this process, acquisition adaptive exquisitely sensitive inhibition CDK8/19 Mediator kinase, pleiotropic regulator transcriptional reprogramming. Our data demonstrate after from direct contributor metastasis kinase during adaptation provides therapeutic opportunity prevent both local cancer.

Language: Английский

Advancing sustainable agriculture: a critical review of smart and eco-friendly nanomaterial applications DOI Creative Commons
Sri Renukadevi Balusamy, Abhayraj S. Joshi, Haribalan Perumalsamy

et al.

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Oct. 11, 2023

Undoubtedly, nanoparticles are one of the ideal choices for achieving challenges related to bio sensing, drug delivery, and biotechnological tools. After gaining success in biomedical research, scientists exploring various types sustainable agriculture. The active can be used as a direct source micronutrients or delivery platform delivering bioactive agrochemicals improve crop growth, yield, quality. Till date, several reports have been published showing applications nanotechnology For instance, methods employed application nanoparticles; especially metal physicochemical properties such core synthesize nanoparticles, their size, shape, surface chemistry, coatings affect crops, soil health, crop-associated ecosystem. Therefore, selecting with appropriate applying them agriculture via suitable method stands smart option achieve improved plant performance. In presented review, we compared nanoparticle plants critically interpreted significant differences find out relatively safe specific agricultural practice. Further, analyzed discussed different that influence on terms nano safety nanotoxicity. From literature would like point implementation smaller sized low concentration seed priming foliar spray could safer minimizing nanotoxicity, exhibiting better performance during stress non-stressed conditions. Moreover, using nanomaterials pose alternative conventional chemical fertilizers cleaner technology While reviewing all available literature, came across some serious drawbacks lack proper regulatory bodies control usage poor knowledge long-term impact ecosystem which need addressed near future comprehensive applicability green

Language: Английский

Citations

50
Priestia megaterium cells are primed for surviving lethal doses of antibiotics and chemical stress DOI Creative Commons

Manisha Guha,

Abhyudai Singh, Nicholas C. Butzin

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: Feb. 8, 2025

Antibiotic resistant infections kill millions worldwide yearly. However, a key factor in recurrent is antibiotic persisters. Persisters are not inherently antibiotic-resistant but can withstand exposure by entering non-dividing state. This tolerance often results prolonged usage, increasing the likelihood of developing strains. Here, we show existence "primed cells" Gram-positive bacterium Priestia megaterium, formerly known as Bacillus megaterium. These cells pre-adapted to become persisters prior lethal stress. Remarkably, this prepared state passed down through multiple generations via epigenetic memory, enhancing survival against antibiotics and other chemical Previously, two distinct types were proposed: Type I II, formed during stationary log phases, respectively. our findings reveal that primed contribute an increase transition with no evidence supporting phenotypes between II

Language: Английский

Citations

1
Drug tolerance and persistence in bacteria, fungi and cancer cells: Role of non-genetic heterogeneity DOI Creative Commons
Imane El Meouche, Paras Jain, Mohit Kumar Jolly

et al.

Translational Oncology, Journal Year: 2024, Volume and Issue: 49, P. 102069 - 102069

Published: Aug. 8, 2024

Language: Английский

Citations

6
Mixed Consortium of Salt-Tolerant Phosphate Solubilizing Bacteria Improves Maize (Zea mays) Plant Growth and Soil Health Under Saline Conditions DOI

Prabhat K. Chauhan,

Sudhir K. Upadhyay

Molecular Biotechnology, Journal Year: 2023, Volume and Issue: 66(3), P. 489 - 499

Published: May 27, 2023

Language: Английский

Citations

12
A heritable iron memory enables decision-making in Escherichia coli DOI Creative Commons
Souvik Bhattacharyya, Nabin Bhattarai, Dylan M. Pfannenstiel

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(48)

Published: Nov. 21, 2023

The importance of memory in bacterial decision-making is relatively unexplored. We show here that a prior experience swarming remembered when Escherichia coli encounters new surface, improving its future efficiency. conducted >10,000 single-cell swarm assays to discover cells store the form cellular iron levels. This “iron” preexists planktonic cells, but act reinforces it. A cell with low initiates early and better swarmer, while opposite true for high iron. potential mother cell, which tracks memory, passed down fourth-generation daughter cells. naturally lost by seventh generation, artificially manipulating levels allows it persist much longer. mathematical model time-delay component faithfully recreates observed dynamic interconversions between different potentials. demonstrate also track biofilm formation antibiotic tolerance, suggesting may impact other physiologies.

Language: Английский

Citations

12
Harnessing the action mechanisms of microbial endophytes for enhancing plant performance and stress tolerance: current understanding and future perspectives DOI
Gursharan Kaur, Arvind Kumar Patel, Vagish Dwibedi

et al.

Archives of Microbiology, Journal Year: 2023, Volume and Issue: 205(9)

Published: Aug. 10, 2023

Language: Английский

Citations

11
Genomic insights and anti-phytopathogenic potential of siderophore metabolome of endolithic Nocardia mangyaensis NH1 DOI Creative Commons
Irina V. Khilyas, Maria Markelova,

Liia R. Valeeva

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 7, 2024

Actinobacteria are one of the predominant groups that successfully colonize and survive in various aquatic, terrestrial rhizhospheric ecosystems. Among actinobacteria, Nocardia is most important agricultural industrial bacteria. Screening isolation related bacteria from extreme habitats such as endolithic environments beneficial for practical applications environmental biotechnology. In this work, bioinformatics analysis revealed a novel strain mangyaensis NH1 has capacity to produce structurally varied bioactive compounds, which encoded by non-ribosomal peptide synthases (NRPS), polyketide synthase (PKS), post-translationally modified peptides (RiPPs). NRPS, five gene clusters have sequence homology with encoding siderophore synthesis. We also show N. accumulates both catechol- hydroxamate-type siderophores simultaneously under iron-deficient conditions. Untargeted LC-MS/MS variety metabolites, including siderophores, lipopeptides, cyclic peptides, indole-3-acetic acid (IAA) culture medium grown iron deficiency. demonstrate four CAS (chrome azurol S)-positive fractions display variable affinity metals, high Fe

Language: Английский

Citations

4
Preexisting cell state rather than stochastic noise confers high or low infection susceptibility of human lung epithelial cells to adenovirus DOI Creative Commons
Anthony Petkidis, Maarit Suomalainen, Vardan Andriasyan

et al.

mSphere, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 24, 2024

ABSTRACT Viruses display large variability across all stages of their life cycle, including entry, gene expression, replication, assembly, and egress. We previously reported that the immediate early adenovirus (AdV) E1A transcripts accumulate in human lung epithelial A549 cancer cells with high variability, mostly independent number incoming viral genomes, but somewhat correlated to cell cycle state at time inoculation. Here, we leveraged classical Luria–Delbrück fluctuation analysis address whether infection primarily arises from or stochastic noise. The expression was measured by green fluorescent protein (GFP) endogenous promoter AdV-C5_E1A-FS2A-GFP found be highly plaque formation, indicating reliability reporter virus. As an ensemble, randomly picked clonal isolates displayed significantly higher coefficients variation than technical noise, a phenotypic larger underlying determining maintained for least 9 weeks cultivation. Our results indicate preexisting states tune favor These findings have implications antiviral strategies therapy applications. IMPORTANCE Viral infections are known variability. Underlying mechanisms still incompletely understood been associated particular states, example, eukaryotic division DNA virus infections. A is collective biochemical, morphological, contextual features owing conditions random. It affects how intrinsic extrinsic cues trigger response, such as anti-viral state. provide evidence built-in memory confer low susceptibility infection. Results reminiscent test bacteriophage back 1943, which demonstrated mutations, absence selective pressure prior infection, cause resistance rather being consequence dynamic conferring uncover new challenges prediction treatment

Language: Английский

Citations

4
Colorectal cancer in Lynch syndrome families: consequences of gene germline mutations and the gut microbiota DOI Creative Commons
Xuexin Wang,

Zhijun Zheng,

Dongliang Yu

et al.

Orphanet Journal of Rare Diseases, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 18, 2025

Lynch syndrome (LS)-associated colorectal cancer (CRC) always ascribes to pathogenic germline mutations in mismatch repair (MMR) genes. However, the penetrance of CRC varies among those with same MMR gene mutation. Thus, we hypothesized that gut microbiota is also involved development LS families. This prospective, observational study was performed from December 2020 March 2023. We enrolled 72 individuals 9 families across six provinces China and employed 16S rRNA amplicon sequencing analyze fecal components LS-related patients (AS group), their spouses (BS mutation carriers without (CS non-mutation (DS group) using alpha beta diversity indices. There were no apparent differences age or gender four groups. Alpha indices exhibited significant between AS BS groups, verifying role occurrence Beta analysis CS revealing importance for A greater difference (both indices) shown DS demonstrating combined impact genetic on Compared identified ten microbial genera enriched group, one genus (Bacteroides) decreased group. Among elevated Agathobacter, Coprococcus Prevotellaceae_NK3B31_group butyrate-producing genera. found can be attributed effects as well provided novel insights into prevention treatment

Language: Английский

Citations

0
Criticality in the Luria-Delbrück Model with an Arbitrary Mutation Rate DOI
Deng Pan, Jie Lin, Ariel Amir

et al.

Physical Review Letters, Journal Year: 2025, Volume and Issue: 134(3)

Published: Jan. 22, 2025

The Luria-Delbrück model is a classic of population dynamics with random mutations, that has been used historically to prove mutations drive evolution. In typical scenarios, the relevant mutation rate exceedingly small, and mutants are counted only at final time point. Here, inspired by recent experiments on DNA repair, we study mathematical formally equivalent setup, repair p playing role rate, albeit taking large values, order unity per cell division. We find although times fraction repaired cells approaches one, variance number undergoes phase transition: when p>1/2 decreases time, but, intriguingly, for p<1/2 even though 1, in increases time. Analyzing DNA-repair experiments, explain data should also take into account probability successful process once it initiated. Taken together, our work shows how variability can lead surprising transitions as well provide biological insights repair.

Language: Английский

Citations

0