Impact of pollution on microbiological dynamics in the pistil stigmas of Orobanche lutea flowers (Orobanchaceae)

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 27, 2025

Our understanding of the basic relationships microbiota associated with flowers is still quite limited, especially regarding parasitic plant species. The transient nature flower parts such as pistil stigmas provides a unique opportunity for temporal investigations. This first report analysis bacterial and fungal communities lucerne parasite, Orobanche lutea. We compared microorganism at different developmental stages assessed impact pollution sampling sites. also examined growth properties (PGP) bacteria in culture-dependent analysis. predominant colonizers were Proteobacteria (99.25%), Enterobacteriaceae (49.88%) Pseudomonadaceae (48.28%) being major families. prevalent phylum was Basidiomycota (71.64%), Filobasidiales (33.14%) Tremellales (27.27%) dominant orders. Microbial populations polluted area showed increased diversity. Mature exhibited greater microbial variety to immature ones. found higher than abundance both unpolluted In analysis, from had least morphotypes. Identified culturable represented Acinetobacter, Erwinia, Micrococcus, Oceanobacillus, Pantoea, Pseudomonas, Serratia, Staphylococcus genera. assessment PGP traits revealed multiple strains growth-promoting potential. composition varied between sites influenced by flower's stage.

Language: Английский

Carvacrol Treatment Reduces Decay and Maintains the Postharvest Quality of Red Grape Fruits (Vitis vinifera L.) Inoculated with Alternaria alternata

Foods, Journal Year: 2023, Volume and Issue: 12(23), P. 4305 - 4305

Published: Nov. 29, 2023

In this study, we isolated and identified pathogenic fungi from the naturally occurring fruits of red grapes, studied their biological characteristics, screened fifteen essential oil components to find best natural antibacterial agent with strongest inhibitory effect, then compared incidence postharvest diseases storage potential grapes treated two concentrations (0.5 EC50/EC50) (inoculated fungi) during for 12 d at room temperature. our research, Alternaria alternata was primary fungus grapes. Specifically, became infected which caused diseases, regardless whether they were inoculated in an injured or uninjured state. Our findings demonstrated that following conditions ideal mycelial development spore germination: BSA medium, D-maltose, ammonium nitrate, 28 °C, pH 6, exposure light. For production, OA mannitol, urea, 34 9, dark advised. Furthermore, EC50 value 36.71 μg/mL, carvacrol highest impact on among 15 oils. meantime, treatment concentration found be more effective than 0.5 controlling alternata-induced decay disease The exhibited remarkable improvements activity defense-related enzymes, preservation greatest hardness total soluble solids content, reduction membrane lipid peroxidation peel, structural integrity peel cells. Consequently, able prevent infestation affects its produced outcomes.

Language: Английский

Comparative assessment of UV-C radiation and non-thermal plasma for inactivation of foodborne fungal spores suspension in vitro

RSC Advances, Journal Year: 2024, Volume and Issue: 14(24), P. 16835 - 16845

Published: Jan. 1, 2024

The comparative efficacy of UV radiation and non-thermal plasma (NTP) on key foodborn fungal contaminants was studied. UV-C (254 nm) did not significantly suppress the metabolic activity cells are spore germination whereas NTP exhibited almost 100% effectiveness.

Language: Английский