Antibiotic Resistance in Plant Pathogenic Bacteria: Recent Data and Environmental Impact of Unchecked Use and the Potential of Biocontrol Agents as an Eco-Friendly Alternative

Plants, Journal Year: 2024, Volume and Issue: 13(8), P. 1135 - 1135

Published: April 18, 2024

The economic impact of phytopathogenic bacteria on agriculture is staggering, costing billions US dollars globally.

Language: Английский

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On the use of antibiotics to control plant pathogenic bacteria: a genetic and genomic perspective

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: June 27, 2023

Despite growing attention, antibiotics (such as streptomycin, oxytetracycline or kasugamycin) are still used worldwide for the control of major bacterial plant diseases. This raises concerns on their potential, yet unknown impact antibiotic and multidrug resistances spread genetic determinants among pathogens. Antibiotic resistance genes (ARGs) have been identified in pathogenic bacteria (PPB), with streptomycin being most commonly reported. Therefore, contribution mobile elements (MGEs) to PPB, well ability transfer other bacteria, need be further explored. The only well-documented example ARGs vector Tn 5393 its highly similar variants (carrying genes), is concerning because presence outside Salmonella enterica Klebsiella pneumoniae , two human Although structure PPB relatively simple, human- animal-associated has evolved into complex associations MGEs ARGs. review sheds light associated but also investigates potential role use selection plant-associated bacteria.

Language: Английский

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The Use and Impact of Antibiotics in Plant Agriculture: A Review

Phytopathology, Journal Year: 2024, Volume and Issue: 114(5), P. 885 - 909

Published: March 13, 2024

Growers have depended on the specificity and efficacy of streptomycin oxytetracycline as a part their plant disease arsenal since middle 20th century. With climate change intensifying bacterial epidemics, established success these antibiotics remains threatened. Our strong reliance certain for devastating diseases eventually gave way to resistance development. Although in agriculture equal less than 0.5% overall antibiotic use United States, it is still imperative humans continue monitor usage, environmental residues, populations. This review provides an overview history use, mitigation, regulation, impact, economics agriculture. Bacterial issues, such ongoing Huanglongbing (citrus greening) epidemic Florida citrus production, may need adequate control. Therefore, preserving our current by utilizing more targeted application methods, trunk injection, should be major focus. [Formula: see text] Copyright © 2024 The Author(s). open access article distributed under CC BY 4.0 International license .

Language: Английский

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Uptake of the Plant Agriculture-Used Antibiotics Oxytetracycline and Streptomycin by Cherry Radish─Effect on Plant Microbiome and the Potential Health Risk

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(11), P. 4561 - 4570

Published: March 9, 2023

Antibiotics are used to control certain bacterial diseases in plant agriculture. Understanding antibiotic uptake by edible vegetables after application and associated risks on microbiome human health is critical. In this study, oxytetracycline streptomycin, the two most commonly antibiotics agriculture, were applied cherry radish via continuous soil drenching study their translocations into tissues, influence microbiome, potential risk mice. The results demonstrated that induced hormesis plants both translocated leaves, fruits, roots of radishes from soil, with significantly higher streptomycin than oxytetracycline. Interestingly, proportion culturable or streptomycin-resistant bacteria antibiotic-accumulated tissues was antibiotic-free although fungal communities different not affected accumulated antibiotics, demonstrating could enrich resistance microbiome. Feeding mice antibiotics-accumulated did show significant effects weight blood glucose levels Overall, provides important insights using

Language: Английский

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Data collection on antibiotics for control of plant pathogenic bacteria

EFSA Supporting Publications, Journal Year: 2024, Volume and Issue: 21(1)

Published: Jan. 1, 2024

There are neither guidance nor methodology proposed to systematically collect data on antibiotics used as plant protection products, antimicrobial resistance of pathogenic bacteria or alternative and innovative treatments for the control phytopathogenic bacteria, at worldwide scale level. This is final report project collection with view reducing risk assessment uncertainties. The collected reviewed scientific, grey patent literature information. analysis highlighted lack publicly easily accessible products. On a scale, up 39 countries have been found using such kasugamycin, gentamicin, streptomycin, oxolinic acid, oxytetracycline, validamycin zhongshengmycin. also pointed out i) change use over time dependent practice legislation changes ii) question about animal human health lying in production possibility select complex antibiotic gene vectors. Streptomycin which most often reported resistant, via several ways counteracting effect this antibiotic. was 18 countries. Globally reports arose mainly from USA, South America Asia. measures, few already commercially available sometimes providing efficient but mostly lot under research development. Data gaps, uncertainties needs improve were highlighted. Finally, stressed need raise awareness improper an emphasis capacity building communication.

Language: Английский

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Biological Control of Tomato Bacterial Leaf Spots and Its Impact on Some Antioxidant Enzymes, Phenolic Compounds, and Pigment Content

Biology, Journal Year: 2024, Volume and Issue: 13(6), P. 369 - 369

Published: May 23, 2024

Tomato bacterial spots, caused by Xanthomonas campestris pv. vesicatoria (Xcv1) and X. euvesicatoria (Xe2), as well specks, two strains of Pseudomonas syringae tomato (Pst1 Pst2), represent significant threats to production in the El-Sharkia governorate, often resulting substantial yield losses. The objective this study was evaluate efficacy various biocontrol culture filtrates, including bacteria fungi agents, managing occurrence severity these diseases, while also monitoring physiological changes leaves, antioxidant enzymes, phenolics, pigment content. filtrates from examined Trichoderma species (T. viride, T. harzianum, album), tested (Bacillus subtilis, fluorescens, Serratia marcescens) at concentrations 25%, 50%, 100%, significantly inhibited proliferation pathogenic In vitro. For vivo experiments, we used specific doses 5 mL spore suspension per plant for fungal bioagents a concentration 2.5 × 107 spores/mL. were applied 10 1 108 CFU/mL. Spraying days before infection reduced disease incidence severity. viride exhibited highest among bioagents, followed harzianum album. Meanwhile, filtrate B. subtilis emerged most potent P. fluorescens. Furthermore, applying resulted elevated levels chitinase, peroxidase, polyphenol oxidase activity. This effect extended increased phenol contents, chlorophyll a, b, carotenoids sprayed plants compared control treatment. Overall, findings underscore potential strategies effectively mitigate enhancing defense mechanisms parameters, thus offering promising avenues sustainable management production.

Language: Английский

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Non-Canonical Aspects of Antibiotics and Antibiotic Resistance

Antibiotics, Journal Year: 2024, Volume and Issue: 13(6), P. 565 - 565

Published: June 17, 2024

The understanding of antibiotic resistance, one the major health threats our time, is mostly based on dated and incomplete notions, especially in clinical contexts. “canonical” mechanisms action pharmacodynamics antibiotics, as well methods used to assess their activity upon bacteria, have not changed decades; same applies definition, acquisition, selective pressures, drivers resistance. As a consequence, strategies improve usage overcome resistance ultimately failed. This review gathers most “non-canonical” notions antibiotics resistance: from alternative limitations susceptibility testing wide variety lateral gene transfer mechanisms, ubiquity, societal factors maintaining Only by having “big picture” view problem can adequate harness be devised. These must global, addressing many aspects that drive increasing prevalence resistant bacteria aside use antibiotics.

Language: Английский

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A Novel IncX Plasmid Mediates High-Level Oxytetracycline and Streptomycin Resistance in Erwinia amylovora from Commercial Pear Orchards in California

Phytopathology, Journal Year: 2023, Volume and Issue: 113(12), P. 2165 - 2173

Published: Aug. 11, 2023

Isolates of the fire blight pathogen Erwinia amylovora with high-level resistance to oxytetracycline (minimal inhibitory concentration [MIC] > 100 μg/ml) and streptomycin (MIC were recovered from four commercial pear orchards in California between 2018 2020. The two representative oxytetracycline- streptomycin-resistant (OxyTcR-SmR) strains 32-10 33-1 as virulent antibiotic susceptible strain 13-1 causing blossom more than 50% time 7 days after co-inoculation flowers 13-1. In field, inoculation that pretreated at 200 μg/ml did not reduce disease compared an untreated control. Four OxyTcR-SmR subjected draft genome sequencing identify genetic determinants their location. A 43.6-kb IncX plasmid, designated pX11-7, was detected each strains, this plasmid encoded tetracycline-resistance gene tetB streptomycin-resistance pair strAB within a large putatively mobile element consisting transposon Tn10 had inserted Tn6082. We also determined pX11-7 conjugative transferred rate 104 105 higher into E. isolated Michigan. occurrence high levels both significantly limits options for management these locations.

Language: Английский

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Antibacterial effect of essential oils and their components against Xanthomonas arboricola pv. pruni revealed by microdilution and direct bioautographic assays

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: June 14, 2023

Bacterial spot of stone fruits caused by Xanthomonas arboricola pv. pruni (Xap) is one the most significant diseases several Prunus species. Disease outbreaks can result in severe economic losses while control options are limited. Antibacterial efficacy essential oils (EOs) thyme, cinnamon, clove, rosemary, tea tree, eucalyptus, lemon grass, citronella and balm was assessed against two Hungarian Xap isolates. The minimal inhibitory concentration (MIC) determined broth microdilution assay for identification active EOs' components a newly introduced high-performance thin-layer chromatography (HPTLC)-Xap (direct bioautography) method combined with solid-phase microextraction-gas chromatography/mass spectrometry (SPME-GC/MS) applied. All EOs inhibited both bacterium isolates, but cinnamon proved to be effective EO MIC values 31.25 µg/mL 62.5 µg/mL, respectively. Compounds antibacterial HPTLC zones were identified as thymol trans-cinnamaldehyde eugenol borneol terpinen-4-ol citral (neral geranial) grass balm, citronellal nerol grass. Regarding compounds, had highest efficiency value 50 µg/mL. effects have already been proven species, our knowledge, studied EOs, except tested first time Xap. Furthermore, case Xap, this report demonstrating that direct bioautography fast suitable screening anti-Xap complex matrices, like EOs.

Language: Английский

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Development of a long amplicon PMA-qPCR assay for detection of viable Xanthomonas arboricola pv. pruni cells in peach trees

Plant Disease, Journal Year: 2024, Volume and Issue: 108(7), P. 2190 - 2196

Published: March 27, 2024

Bacterial spot is one of the most serious diseases peach caused by pathogen

Language: Английский

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