Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics DOI Creative Commons
Monika Kałużna,

Andjelka Prokić,

Aleksa Obradović

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 13, 2023

Xanthomonas arboricola pv. corylina (Xac; formerly campestris corylina) is the causal agent of bacterial blight hazelnuts, a devastating disease trees in plant nurseries and young orchards. Currently, there are no PCR assays to distinguish Xac from all other pathovars X. arboricola. A comparative genomics approach with publicly available genomes was used identify unique sequences, conserved across pathogen. We identified 2,440 bp genomic region that designed identification detection systems for conventional PCR, qPCR (SYBR® Green TaqMan™), loop-mediated isothermal amplification (LAMP). All performed on DNA isolated eight closely related species confirmed specificity primers. These new multi-platform molecular diagnostic tools may be by clinics researchers detect pure cultures hazelnut tissues rapidly accurately.

Language: Английский

Antimicrobial Residues in Foods DOI
Elisabeth Zeuko’o Menkem

Elsevier eBooks, Journal Year: 2023, Volume and Issue: unknown, P. 603 - 620

Published: May 8, 2023

Language: Английский

Citations

0
Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics DOI Creative Commons
Monika Kałużna,

Andjelka Prokić,

Aleksa Obradović

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 13, 2023

Xanthomonas arboricola pv. corylina (Xac; formerly campestris corylina) is the causal agent of bacterial blight hazelnuts, a devastating disease trees in plant nurseries and young orchards. Currently, there are no PCR assays to distinguish Xac from all other pathovars X. arboricola. A comparative genomics approach with publicly available genomes was used identify unique sequences, conserved across pathogen. We identified 2,440 bp genomic region that designed identification detection systems for conventional PCR, qPCR (SYBR® Green TaqMan™), loop-mediated isothermal amplification (LAMP). All performed on DNA isolated eight closely related species confirmed specificity primers. These new multi-platform molecular diagnostic tools may be by clinics researchers detect pure cultures hazelnut tissues rapidly accurately.

Language: Английский

Citations

0