Seasonal and daily patterns in known dissolved metabolites in the northwestern Sargasso Sea DOI Open Access
Krista Longnecker, Melissa C. Kido Soule, Gretchen J. Swarr

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Dec. 22, 2022

Abstract Organic carbon in seawater plays a significant role the global cycle. The concentration and composition of dissolved organic carbon, operationally defined this project as that passes through 0.2 µm filter, reflect activity biological community chemical reactions occur seawater. From 2016 to 2019, we repeatedly sampled oligotrophic northwest Sargasso Sea vicinity Bermuda Atlantic Time-series Study site (BATS) quantitatively follow known compounds within pool matter upper 1000 meters water column. Dissolved metabolite concentrations revealed patterns with depth time most metabolites showing surface enrichment lower increasing depth. Select displayed seasonal throughout year, seasonality was repeated each years sampled. Concentrations vitamins, including pantothenic acid (vitamin B 5 ) riboflavin 2 ), increased annually during winter periods when mixed layer depths were deepest. During diel sampling, light-sensitive decreased significantly daylight hours. Metabolite over at BATS showed less variability compared previous sample set collected broad latitudinal range western Ocean. examined study are all components central metabolism. By examining these finer resolution relatively long series, have insights into microbial biogeochemical activity, data which fundamental understanding response marine systems future changes climate.

Language: Английский

Determination of dissolved organic carbon and total dissolved nitrogen in seawater using High Temperature Combustion Analysis DOI Creative Commons
Elisa R. Halewood, Keri Opalk,

Lillian Custals

et al.

Frontiers in Marine Science, Journal Year: 2022, Volume and Issue: 9

Published: Dec. 12, 2022

This document describes best practices for analysis of dissolved organic matter (dissolved carbon and total nitrogen) in seawater samples. Included are SOPs sample collection storage, details laboratory using high temperature combustion on Shimadzu TOC analyzers, suggestions quality control assurance. Although written specifically GO-SHIP oceanographic community practices, many aspects processing relevant to DOM determination across oceanic regimes this aims provide updated methodology the wider marine community.

Language: Английский

Citations

46
Seasonal and daily patterns in known dissolved metabolites in the northwestern Sargasso Sea DOI Creative Commons
Krista Longnecker, Melissa C. Kido Soule, Gretchen J. Swarr

et al.

Limnology and Oceanography, Journal Year: 2024, Volume and Issue: 69(3), P. 449 - 466

Published: Jan. 10, 2024

Abstract Organic carbon in seawater plays a significant role the global cycle. The concentration and composition of dissolved organic reflect activity biological community chemical reactions that occur seawater. From 2016 to 2019, we repeatedly sampled oligotrophic northwest Sargasso Sea vicinity Bermuda Atlantic Time‐series Study site (BATS) quantitatively follow known compounds within pool matter upper 1000 m water column. Most metabolites showed surface enrichment, 83% had significantly lower concentrations with increasing depth. Dissolved metabolite most notably revealed temporal variability. Fourteen displayed seasonality was repeated each 4 yr sampled. Concentrations vitamins, including pantothenic acid (vitamin B 5 ) riboflavin 2 ), increased annually during winter periods when mixed layer depths were deepest. During diel sampling, light‐sensitive decreased daylight hours. variability at BATS less than spatial from previous sample set collected over broad latitudinal range western Ocean. examined this study are all components central metabolism. By examining these finer resolution time‐series, begin provide insights into may be exchanged by microorganisms marine systems, data which fundamental understanding response systems future changes climate.

Language: Английский

Citations

5
Relating Molecular Properties to the Persistence of Marine Dissolved Organic Matter with Liquid Chromatography–Ultrahigh-Resolution Mass Spectrometry DOI
Rene Boiteau, Yuri Corilo, William Kew

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 9, 2024

Marine dissolved organic matter (DOM) contains a complex mixture of small molecules that eludes rapid biological degradation. Spatial and temporal variations in the abundance DOM reflect existence fractions are removed from ocean over different time scales, ranging seconds to millennia. However, it remains unknown whether intrinsic chemical properties these components relate their persistence. Here, we elucidate compare molecular compositions distinct with lability along water column North Atlantic Gyre. Our analysis utilized ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry at 21 T coupled liquid chromatography novel data pipeline developed CoreMS generates formula assignments metrics isomeric complexity. Clustering binned 14 857 into groups correspond depth distribution semilabile, semirefractory, refractory DOM. The more labile were concentrated near surface contained aliphatic, hydrophobic, reduced than fraction, which occurred uniformly throughout column. These findings suggest processes selectively remove hydrophobic compounds, such as aggregation particle sorption, contribute variable removal rates marine

Language: Английский

Citations

5
Long-read powered viral metagenomics in the oligotrophic Sargasso Sea DOI Creative Commons
Joanna Warwick-Dugdale, Funing Tian,

Michelle L. Michelsen

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 14, 2024

Dominant microorganisms of the Sargasso Sea are key drivers global carbon cycle. However, associated viruses that shape microbial community structure and function not well characterised. Here, we combined short long read sequencing to survey phage communities in virus- cellular fractions at viral maximum (80 m) mesopelagic (200 depths. We identified 2,301 populations from 186 genera. Over half here lacked representation ocean metagenomes, whilst 177 genera genomic databases isolates. Viral fraction cell-associated were decoupled, indicating turnover occurred across periods longer than sampling period three days. Inclusion long-read data was critical for capturing breadth diversity. Phage isolates infect dominant bacterial taxa Prochlorococcus Pelagibacter, usually regarded as cosmopolitan abundant, poorly represented.

Language: Английский

Citations

4
Recurring seasonality exposes dominant species and niche partitioning strategies of open ocean picoeukaryotic algae DOI Creative Commons
Charlotte Eckmann, Charles Bachy, Fabian Wittmers

et al.

Communications Earth & Environment, Journal Year: 2024, Volume and Issue: 5(1)

Published: May 20, 2024

Abstract Ocean spring phytoplankton blooms are dynamic periods important to global primary production. We document vertical patterns of a diverse suite eukaryotic algae, the prasinophytes, in North Atlantic Subtropical Gyre with monthly sampling over four years at Bermuda Time-series Study site. Water column structure was used delineate seasonal stability more ecologically relevant than seasons defined by calendar dates. During winter mixing, tiny prasinophytes dominated Class II comprise 46 ± 24% algal (plastid-derived) 16S rRNA V1-V2 amplicons, specifically Ostreococcus Clade OII, Micromonas commoda , and Bathycoccus calidus . In contrast, VII rare Classes I VI peak during warm stratified when surface abundances low. Seasonality underpins reservoir genetic diversity from multiple prasinophyte classes that harbor ephemeral taxa. Persistent sub-species dominating winter/spring bloom period retreat deep chlorophyll maximum summer, poised seed mixed layer upon convection, exposing mechanism for initiating high onset. Comparisons tropical oceans reveal broad distributions dominant herein. This unparalleled window into temporal spatial niche partitioning picoeukaryotic producers demonstrates how key prevail oceans.

Language: Английский

Citations

4
DOM production, removal, and transformation processes in marine systems DOI
Craig A. Carlson, Shuting Liu, Brandon M. Stephens

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 137 - 246

Published: Jan. 1, 2024

Language: Английский

Citations

4
Metabolite diversity among representatives of divergent Prochlorococcus ecotypes DOI Creative Commons
Elizabeth B. Kujawinski, Rogier Braakman, Krista Longnecker

et al.

mSystems, Journal Year: 2023, Volume and Issue: 8(5)

Published: Oct. 10, 2023

ABSTRACT The euphotic zone of the surface ocean contains distinct physical-chemical regimes that vary in light and nutrient concentrations as an inverse function depth. most numerous phytoplankter mid- low-latitude is picocyanobacterium Prochlorococcus , which consists ecologically subpopulations (i.e., “ecotypes”). Ecotypes have different temperature, light, optima display relative abundances along gradients these niche dimensions. As a primary producer, fixes releases organic carbon to neighboring microbes part microbial loop. However, little known about specific molecules accumulates or how processes among its ecotypes. Here, we characterize metabolite diversity by profiling three cultured strains: MIT9301, representing high-light-adapted ecotype dominating shallow tropical sub-tropical waters; MIT0801, low-light-adapted found throughout zone; MIT9313, relatively abundant at base zone. In both intracellular extracellular profiles, observe striking differences across strains accumulation release molecules, such DNA methylating agent S-adenosyl-methionine (intracellular) branched-chain amino acids their precursors (extracellular). While some reflect variable genome content strains, others likely regulation conserved pathways. identify pantothenic acid aromatic may serve currencies ’ interactions with and, therefore, merit further investigation. IMPORTANCE Approximately half annual fixation on Earth occurs through photosynthetic activities phytoplankton ubiquitous . Ecologically (or ecotypes) are central conduits substrates into microbiome, thus playing important roles production. We measured chemical profile observing them implications for impact surroundings wild. Subpopulations differ abundance concentrations, suggesting could affect cycling strata should be considered models physiology marine dynamics.

Language: Английский

Citations

10
Predictable functional biogeography of marine microbial heterotrophs DOI Creative Commons
Emily J. Zakem, Jesse McNichol, JL Weissman

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 15, 2024

Abstract Heterotrophic bacteria and archaea (‘heteroprokaryotes’) drive global carbon cycling, but how to quantitatively organize their functional complexity remains unclear. We generated a global-scale understanding of marine heteroprokaryotic biogeography by synthesizing genetic sequencing data with mechanistic ecosystem model. incorporated diversity into the trait-based model along two axes: substrate lability growth strategy. Using sequences three ocean transects, we compiled 21 guilds estimated degree optimization for rapid (copiotrophy). Data consistency indicated that gradients in grazing predominantly set biogeographical patterns, identified deep-ocean ‘slow copiotrophs’ whose ecological interactions control surface accumulation dissolved organic carbon.

Language: Английский

Citations

2
A data-driven approach for predicting depth-averaged velocities in the early stages of underwater glider navigation DOI
Hualing Li, Yaojian Zhou, Yuning Zhao

et al.

Ocean Engineering, Journal Year: 2024, Volume and Issue: 299, P. 117417 - 117417

Published: March 11, 2024

Language: Английский

Citations

2
Glucose Enhances Salinity-Driven Sea Spray Aerosol Production in Eastern Arctic Waters DOI Creative Commons
Arianna Rocchi, Anabel von Jackowski, André Welti

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(20), P. 8748 - 8759

Published: May 6, 2024

Sea spray aerosols (SSA) greatly affect the climate system by scattering solar radiation and acting as seeds for cloud droplet formation. The ecosystems in Arctic Ocean are rapidly changing due to global warming, effects these changes have on generation of SSA, thereby clouds fog formation this region, unknown. During ship-based Century Expedition, we examined dependency forced SSA production biogeochemical characteristics seawater using an on-board temperature-controlled aerosol chamber with a plunging jet system. Our results indicate that mainly salinity organic content influence size distribution SSA. However, observed 2-fold higher from waters similar collected north 81°N compared samples south latitude. This variability was not explained phytoplankton bacterial abundances or Chlorophyll-a concentration but presence glucose seawater. synergic action sea salt (essential component) glucose-rich saccharides (enhancer) accounts >80% predictability throughout cruise. suggest besides wind speed salinity, is also affected specific organics released microbiota.

Language: Английский

Citations

2