Effects of Inducible Nitric Oxide Synthase (iNOS) Gene Knockout on the Diversity, Composition, and Function of Gut Microbiota in Adult Zebrafish DOI Creative Commons

Yajuan Huang,

Yadong Chen, Haisheng Xie

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(6), P. 372 - 372

Published: May 23, 2024

The gut microbiota constitutes a complex ecosystem that has an important impact on host health. In this study, genetically engineered zebrafish with inducible nitric oxide synthase (iNOS or NOS2) knockout were used as model to investigate the effects of nos2a/nos2b gene single and nos2 double intestinal microbiome composition function. Extensive 16S rRNA sequencing revealed substantial changes in microbial diversity specific taxonomic abundances, yet it did not affect functional structure tissues. Notably, iNOS-deficient demonstrated decrease Vibrio species increase Aeromonas species, more pronounced observed knockouts. Further transcriptomic analysis iNOS indicated significant alterations immune-related metabolic pathways, including complement PPAR signaling pathways. These findings underscore crucial interplay between genetics microbiota, indicating plays key role modulating ecology, immune system, responses.

Language: Английский

The uses of zebrafish (Danio rerio) as an in vivo model for toxicological studies: A review based on bibliometrics DOI Creative Commons
Weichao Zhao,

Y.-W. Chen,

Nan Hu

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 272, P. 116023 - 116023

Published: Jan. 30, 2024

An in vivo model is necessary for toxicology. This review analyzed the uses of zebrafish (Danio rerio) toxicology based on bibliometrics. Totally 56,816 publications about from 2002 to 2023 were found Web Science Core Collection, with Toxicology as top 6 among all disciplines. Accordingly, bibliometric map reveals that "toxicity" has become a hot keyword. It further most common exposure types include acute, chronic, and combined exposure. The toxicological effects behavioral, intestinal, cardiovascular, hepatic, endocrine toxicity, neurotoxicity, immunotoxicity, genotoxicity, reproductive transgenerational toxicity. mechanisms oxidative stress, inflammation, autophagy, dysbiosis gut microbiota. toxicants commonly evaluated by using nanomaterials, arsenic, metals, bisphenol, dioxin. Overall, provide unique well-accepted investigate mechanisms. We also discussed possible ways address some limitations model, such combination human organoids avoid species differences.

Language: Английский

Citations

34
Zebrafish: an efficient vertebrate model for understanding role of gut microbiota DOI Creative Commons
Hui Xia, Huimin Chen, Xue Cheng

et al.

Molecular Medicine, Journal Year: 2022, Volume and Issue: 28(1)

Published: Dec. 1, 2022

Abstract Gut microbiota plays a critical role in the maintenance of host health. As low-cost and genetically tractable vertebrate model, zebrafish have been widely used for biological research. Zebrafish humans share some similarities intestinal physiology function, this allows to be surrogate model investigating crosstalk between gut host. Especially, features such as high fecundity, external fertilization, early optical transparency. These enable researchers employ fish address questions not easily addressed other animal models. In review, we described intestine structure zebrafish. Also, summarized methods generating gnotobiotic factors affecting its flora, study progress functions Finally, discussed limitations challenges studies. summary, review established that is an attractive research tool understand mechanistic insights into host-microbe interaction.

Language: Английский

Citations

47
Effects of the plastic additive 2,4-di-tert-butylphenol on intestinal microbiota of zebrafish DOI
Yongmeng Yang,

Chen Yan,

Aifeng Li

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 469, P. 133987 - 133987

Published: March 8, 2024

Language: Английский

Citations

9
High-throughput sequencing of 16S rRNA gene analysis reveal insights into abundance, diversity, and function of the gut microbiota in zebrafish exposed to triflumezopyrim DOI
Ipsita Iswari Das,

Bayasis M. Sharma,

Madhusmita Mahapatra

et al.

Aquaculture Reports, Journal Year: 2025, Volume and Issue: 40, P. 102651 - 102651

Published: Jan. 25, 2025

Language: Английский

Citations

1
Zebrafish: A trending model for gut-brain axis investigation DOI

Neelakanta Sarvashiva Kiran,

Chandrashekar Yashaswini,

Ankita Chatterjee

et al.

Aquatic Toxicology, Journal Year: 2024, Volume and Issue: 270, P. 106902 - 106902

Published: March 17, 2024

Language: Английский

Citations

6
Exploring the impact of antibiotics, microplastics, nanoparticles, and pesticides on zebrafish gut microbiomes: Insights into composition, interactions, and health implications DOI
Rajesh Pamanji,

T.N. Kumareshan,

Lakshmi Priya S

et al.

Chemosphere, Journal Year: 2023, Volume and Issue: 349, P. 140867 - 140867

Published: Dec. 2, 2023

Language: Английский

Citations

12
Zebrafish as a model for human epithelial pathology DOI Creative Commons
Ahmed Abu‐Siniyeh,

Moayad Khataibeh,

Walid Al-Zyoud

et al.

Laboratory Animal Research, Journal Year: 2025, Volume and Issue: 41(1)

Published: Feb. 3, 2025

Abstract Zebrafish ( Danio rerio ) have emerged as an influential model for studying human epithelial pathology, particularly because of their genetic similarity to humans and unique physiological traits. This review explores the structural functional homology between zebrafish tissues in organs, such gastrointestinal system, liver, kidneys. possess significant cellular with mammals, which facilitates investigation various diseases, including inflammatory bowel disease, nonalcoholic fatty liver polycystic kidney disease. The advantages using a organism include rapid external development, ease manipulation, advanced imaging capabilities, allowing real-time observation disease processes. However, limitations exist, concerning lack organs potential incomplete phenocopy conditions. Despite these challenges, ongoing research adult promises enhance our understanding mechanisms regenerative By revealing similarities differences cell function pathways, this highlights value translational advancing knowledge health developing targeted therapies.

Language: Английский

Citations

0
Protein absorption in the zebrafish gut is regulated by interactions between lysosome rich enterocytes and the microbiome DOI Open Access
Laura Childers, Jieun Park, Siyao Wang

et al.

Published: Feb. 10, 2025

Dietary protein absorption in neonatal mammals and fishes relies on the function of a specialized conserved population highly absorptive lysosome rich enterocytes (LREs). The gut microbiome has been shown to enhance nutrients, such as lipids, by intestinal epithelial cells. However, whether is also affected poorly understood. Here, we investigate connections between microbes zebrafish gut. Using live microscopy-based quantitative assays, find that slow pace uptake degradation LREs. While do not affect number absorbing LRE cells, lower expression endocytic digestion machinery transgene assisted cell isolation single RNA-sequencing, characterize all cells take up dietary protein. We bacteria-sensing metabolic pathways LREs, some secretory types share components with custom-formulated diets, investigated influence diet activity microbiome. Impaired along protein-deficient diet, alters microbial community leads increased abundance bacterial genera have capacity reduce Together, these results reveal diet-dependent reciprocal interactions LREs regulate absorption.

Language: Английский

Citations

0
Microbial Interactions in Rearing Systems for Marine Fish Larvae DOI Creative Commons

Vasiliki Paralika,

Pavlos Makridis

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 539 - 539

Published: Feb. 27, 2025

This article reviews the scientific literature discussing microbial interactions between water microbiota, live food fish larvae immune system and gut biofilm communities in rearing systems for marine larvae. Fish microbiota is first line of defense against opportunistic pathogens, are vulnerable to high mortalities during weeks after hatching. The bacterial colonization a dynamic process influenced by environmental host-related factors. bacteria transferred from eggs can influence composition early stages fish. ingest free-living microorganisms present water, as drink osmoregulation. In aquaculture systems, conventional feeding–rearing protocol consists zooplankton (rotifers, Artemia, copepods). These organisms filter-feeders. Once new environment, they quickly adopt microflora surrounding water. So, similar at time ingestion biofilms may harbor pathogenic serve reservoir those microbes, which colonize column. methods applied study were reviewed.

Language: Английский

Citations

0
Protein absorption in the zebrafish gut is regulated by interactions between lysosome rich enterocytes and the microbiome DOI Creative Commons
Laura Childers, Jieun Park, Siyao Wang

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: March 13, 2025

Dietary protein absorption in neonatal mammals and fishes relies on the function of a specialized conserved population highly absorptive lysosome-rich enterocytes (LREs). The gut microbiome has been shown to enhance nutrients, such as lipids, by intestinal epithelial cells. However, whether is also affected poorly understood. Here, we investigate connections between microbes zebrafish gut. Using live microscopy-based quantitative assays, find that slow pace uptake degradation LREs. While do not affect number absorbing LRE cells, lower expression endocytic digestion machinery transgene-assisted cell isolation single RNA-sequencing, characterize all cells take up dietary protein. We bacteria-sensing metabolic pathways LREs, some secretory types share components with custom-formulated diets, investigated influence diet activity microbiome. Impaired along protein-deficient diet, alters microbial community leads an increased abundance bacterial genera have capacity reduce Together, these results reveal diet-dependent reciprocal interactions LREs regulate absorption.

Language: Английский

Citations

0