Biology,
Journal Year:
2024,
Volume and Issue:
13(6), P. 372 - 372
Published: May 23, 2024
The
gut
microbiota
constitutes
a
complex
ecosystem
that
has
an
important
impact
on
host
health.
In
this
study,
genetically
engineered
zebrafish
with
inducible
nitric
oxide
synthase
(iNOS
or
NOS2)
knockout
were
used
as
model
to
investigate
the
effects
of
nos2a/nos2b
gene
single
and
nos2
double
intestinal
microbiome
composition
function.
Extensive
16S
rRNA
sequencing
revealed
substantial
changes
in
microbial
diversity
specific
taxonomic
abundances,
yet
it
did
not
affect
functional
structure
tissues.
Notably,
iNOS-deficient
demonstrated
decrease
Vibrio
species
increase
Aeromonas
species,
more
pronounced
observed
knockouts.
Further
transcriptomic
analysis
iNOS
indicated
significant
alterations
immune-related
metabolic
pathways,
including
complement
PPAR
signaling
pathways.
These
findings
underscore
crucial
interplay
between
genetics
microbiota,
indicating
plays
key
role
modulating
ecology,
immune
system,
responses.
Current Developments in Nutrition,
Journal Year:
2023,
Volume and Issue:
7(4), P. 100065 - 100065
Published: March 9, 2023
Healthy
and
predictable
physiologic
homeostasis
is
paramount
in
animal
models
for
biomedical
research.
Proper
macronutrient
intake
an
essential
controllable
environmental
factor
maintaining
health
promoting
experimental
reproducibility.
Evaluate
reductions
dietary
composition
on
body
weight
metrics,
composition,
gut
microbiome
Danio
rerio.
D.
rerio
were
fed
reference
diets
deficient
either
protein
or
lipid
content
14
weeks.
Diets
of
reduced-protein
reduced-fat
resulted
lower
gain
than
the
standard
diet
male
female
Females
had
increased
total
lipid,
suggesting
adiposity
compared
with
females
diet.
In
contrast,
decreased
The
microbial
community
displayed
high
abundances
Aeromonas,
Rhodobacteraceae,
Vibrio.
Vibrio
spp.
dominant
a
diet,
whereas
Pseudomonas
heightened
abundance
when
Predicted
functional
metagenomics
communities
(PICRUSt2)
revealed
3-
to
4-fold
increase
KEGG
(Kyoto
Encyclopedia
Genes
Genomes)
category
steroid
hormone
biosynthesis
both
upregulation
secondary
bile
acid
synthesis
degradation
ketone
bodies
was
concomitant
downregulation
These
study
outcomes
provide
insight
into
future
investigations
understand
nutrient
requirements
optimize
growth,
reproductive,
demographics
populations
metabolism
ecosystem.
evaluations
are
critical
understanding
maintenance
steady-state
metabolic
Curr
Dev
Nutr
20xx;x:xx.
Current Opinion in Toxicology,
Journal Year:
2023,
Volume and Issue:
36, P. 100428 - 100428
Published: Aug. 9, 2023
The
effect
of
chemical
pollution
on
the
microbiomes
wildlife
has
been
given
little
attention.
A
new
concept
is
emerging
where
are
vital
to
host
animal
or
plant
health,
and
for
ecosystems.
Data
mainly
mammals,
birds,
fish.
Changing
environmental
conditions
(e.g.,
salinity,
pH,
season)
exposure
chemicals
alter
composition
gill,
gut
skin
microbiomes.
Gut
also
modulated
by
diet,
including
metals,
nanomaterials,
fungicides
microplastics.
However,
a
change
in
microbiome
does
not
necessarily
infer
adverse
effects
host,
with
some
evidence
co-adaptation.
Environmental
risk
assessment
biocides
nanomaterials
should
be
revisited
context
microbiome-host
interactions,
better
protect
Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: May 9, 2024
Introduction
Cassia
seeds,
originating
from
the
mature
seeds
of
leguminous
cassia
species,
possess
pharmacological
effects
attributed
to
their
rich
composition
various
active
ingredients,
notably
anthraquinones.
While
current
research
predominantly
focuses
on
pharmaceutical
extractions,
there
has
been
limited
progress
in
fermentation
studies.
Methods
Our
study
aimed
enhance
content
compounds
such
as
anthraquinones,
flavonoids,
and
polyphenols
using
microbial
techniques.
We
specifically
optimized
a
system
through
single-factor
experimental
design.
Results
The
antioxidant
properties
solution
were
validated
assays
involving
HaCaT
cells
zebrafish.
observed
effective
suppression
inflammatory
reactions
both
RAW264.7
transgenic
zebrafish
by
solution.
Moreover,
significant
inhibition
tyrosinase
activity
melanin
production
was
evident
B16-F10
Positive
outcomes
also
obtained
antibacterial
chick
embryo
experiments.
Discussion
These
findings
highlight
potential
seed
safe
eco-friendly
material
food
chemistry
biomedical
sciences.
Biology,
Journal Year:
2024,
Volume and Issue:
13(6), P. 372 - 372
Published: May 23, 2024
The
gut
microbiota
constitutes
a
complex
ecosystem
that
has
an
important
impact
on
host
health.
In
this
study,
genetically
engineered
zebrafish
with
inducible
nitric
oxide
synthase
(iNOS
or
NOS2)
knockout
were
used
as
model
to
investigate
the
effects
of
nos2a/nos2b
gene
single
and
nos2
double
intestinal
microbiome
composition
function.
Extensive
16S
rRNA
sequencing
revealed
substantial
changes
in
microbial
diversity
specific
taxonomic
abundances,
yet
it
did
not
affect
functional
structure
tissues.
Notably,
iNOS-deficient
demonstrated
decrease
Vibrio
species
increase
Aeromonas
species,
more
pronounced
observed
knockouts.
Further
transcriptomic
analysis
iNOS
indicated
significant
alterations
immune-related
metabolic
pathways,
including
complement
PPAR
signaling
pathways.
These
findings
underscore
crucial
interplay
between
genetics
microbiota,
indicating
plays
key
role
modulating
ecology,
immune
system,
responses.
