Genome Analysis of Pseudomonas viciae G166 Conferring Antifungal Activity in Grapevine

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(6), P. 398 - 398

Published: May 31, 2024

Grapevine (

Language: Английский

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Defining the secondary metabolites in the Pseudomonas protegens PBL3 secretome with antagonistic activity against Burkholderia glumae

Phytopathology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 5, 2024

Rice production worldwide is threatened by the disease bacterial panicle blight (BPB) caused Burkholderia glumae. Despite threat, resources to control this disease, such as completely resistant cultivars or effective chemical methods, are still lacking. However, need has paved way explore biologically based approaches harnessing antimicrobial activities of environmental bacteria. Previously, bacterium Pseudomonas protegens PBL3 was identified a potential biological agent against B. glumae due its activity Such in vitro and planta associated with P. bacteria-free secreted fraction (secretome), although specific molecules responsible for have remained elusive. In work, we advance characterization secretome evaluating selected secondary metabolites predicted genomic sequence addition, using reversed-phase liquid chromatography tandem mass spectrometry enabled us successfully detect quantify pyoluteorin, 2,4-diacetylphloroglucinol, pyochelin. Among those, pyoluteorin 2,4-diacetylphloroglucinol reduced growth vitro, along reducing symptoms BPB planta, suggesting that these compounds could be biopesticides mitigate BPB.

Language: Английский

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Structures and Biosynthesis of Caryoynencins, Unstable Bacterial Polyynes from Pseudomonas protegens Recombinant Expressing the cayG Gene

The Journal of Organic Chemistry, Journal Year: 2023, Volume and Issue: 88(23), P. 16280 - 16291

Published: Nov. 10, 2023

Bacteria in certain genera can produce "bacterial polyynes" that contain a conjugated C≡C bond starting from terminal alkyne. Protegenin A is derivative of octadecanoic acid contains an ene–tetrayne moiety. It was discovered Pseudomonas protegens Cab57 and exhibits strong antioomycete moderate antifungal activity. By introducing cayG, cytochrome P450 gene Burkholderia caryophylli, into P. Cab57, protegenin converted more complex polyynes, caryoynencins A–E. purification method minimized the degradation isomerization established. For first time, as far we know, 1H 13C{1H} NMR signals were completely assigned by analyzing data isolated compounds enriched with [1-13C]- or [2-13C]-acetate. Through structural analysis D/E bioconversion experiments, observed CayG constructs allyl alcohol moiety A–C through sequential hydroxylation, dehydration, hydroxylation. The recombinant strain exhibited stronger activity compared to wild-type strain. This paper proposes stable determination for various bacterial holds promise engineering host production biologically active polyynes.

Language: Английский

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Benzoxazinoids influence rhizosphere establishment and root colonization by PGPB

Published: March 26, 2024

Benzoxazinoids influence rhizosphere establishment and root colonization by PGPBBenzoxazinoids (BXs) form a group of secondary metabolites produced many plants the grass family (Poaceae).Release activation BXs upon pathogen attack strongly suppresses disease pest species foraging herbivorous insects in areal parts plant.At same time, are constitutively set free predominantly during early plant development, where they affect microbial interaction.Hydroxamic acid BX derivatives such as 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA), 2,4dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) 2-hydroxy-4,7-dimethoxy-1,4benzoxazin-3-one (HDMBOA) general more reactive but have shorter half-life than lactam 2-benzoxazolinone (BOA) 6-methoxy-2-benzoxazolinone (MBOA).Regardless, MBOA is efficient at suppressing several fungal pathogens influences rhizospheric interactions over generations plants.Key to understanding plant-microbe symbiosis knowledge about means chemical communication between symbionts, physiological changes those signaling molecules evoke on each symbiont.Therefore, we aimed study mechanisms which an interspecies exchange information precedes initiation establishment.In order gain insight into these processes, investigated how mediate growth promoting bacteria (PGPB) Azospirillum brasilense Ab-V5, Bacillus thuringiensis RZ2MS9, Pantoea agglomerans 33.1 Pseudomonas protegens Pf-5, adverse effect pathogenic Fusarium.We found that bacterial response exogenic was specific for PGPB dose dependent.Curiously, Fusarium strains isolated from non-BX-producing hosts were susceptible low doses, while maize (from BX-producing host) tolerant.Root patterns Ab-V5 Pf-5 studied detail, showing preference crevices hairs primary sites.MBOA did not biofilm formation Arabidopsis roots improved.Finally, results vitro experiments cross validated transcriptomic assays chemotaxis regulatory protein showed relative upregulation 0.05 mM treatment could correlated amount differential expressed genes related production with concentration.The transcriptome however, little affected, consistent previously obtained results.We conclude intermediate concentrations stimulates motile high evokes metabolic switch preparation colonization.

Language: Английский

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Microbial Secondary Metabolites for Modulating Plant Biotic Stress Resistance: Bridging the Lab-Field Gap

Plant Stress, Journal Year: 2024, Volume and Issue: unknown, P. 100720 - 100720

Published: Dec. 1, 2024

Language: Английский

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