International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(8), P. 4437 - 4437
Published: April 18, 2024
The
potato
dry
rot
disease
caused
by
Fusarium
spp.
seriously
reduces
yield
and
threatens
human
health.
However,
potential
biocontrol
agents
cannot
guarantee
the
stability
activity
of
biocontrol.
Here,
18
synthetic
microbial
communities
different
scales
were
constructed,
with
best
effect
on
screened
through
in
vitro
vivo
experiments.
results
show
that
community
composed
Paenibacillus
amylolyticus,
Pseudomonas
putida,
Acinetobacter
calcoaceticus,
Serratia
proteamaculans,
Actinomycetia
bacterium
Bacillus
subtilis
has
activity.
Metabolomics
protoamaculans
interacts
other
member
strains
to
produce
caproic
acid
reduce
index
38.01%.
Furthermore,
mycelial
growth
inhibition
after
treatment
was
77.54%,
flow
cytometry
analysis
showed
living
conidia
rate
11.2%.
This
study
provides
value
for
application
potatoes,
as
well
interaction
mechanisms
between
communities.
Frontiers in Microbiology,
Journal Year:
2023,
Volume and Issue:
14
Published: June 9, 2023
Fusarium
wilt
of
bananas
(FWB)
is
seriously
affecting
the
sustainable
development
banana
industry
and
caused
by
devastating
soil-borne
fungus
oxysporum
f.
sp.
cubense
tropical
race
4
(Foc
TR4).
Biological
control
a
promising
strategy
for
controlling
in
bananas.
We
previously
identified
Streptomyces
hygroscopicus
subsp.
5-4
with
strong
antifungal
activity
against
FWB.
The
most
possible
antimicrobial
mechanism
strain
was
explored
using
metabolomics
approach,
light
microscopy
imaging,
transmission
electron
(TEM).
membrane
integrity
ultrastructure
Foc
TR4
damaged
after
extract
treatment,
which
supported
degradation
mycelium,
soluble
protein
content,
extracellular
reducing
sugar
NADH
oxidase
activity,
malondialdehyde
mitochondrial
potential,
respiratory
chain
complex
enzyme
activity.
extracts
cultivated
at
different
times
were
characterized
liquid
chromatography-mass
spectrometer
(LC-MS).
647
known
metabolites
detected
strains
5-4.
Hygromycin
B,
gluten
exorphin
B4,
torvoside
G,
(z)-8-tetradecenal,
piperitoside,
sarmentosin,
pubescenol,
other
compounds
main
differential
on
fermentation
culture
7
days.
Compared
extracts,
hygromycin
B
inhibited
mycelial
growth
TR4,
EC50
concentration
7.4
μg/mL.
These
results
showed
that
could
destroy
cell
to
inhibit
growth,
may
be
key
active
metabolite.
might
candidate
FWB
provide
scientific
basis
practical
application
as
biological
agent.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(8), P. 4437 - 4437
Published: April 18, 2024
The
potato
dry
rot
disease
caused
by
Fusarium
spp.
seriously
reduces
yield
and
threatens
human
health.
However,
potential
biocontrol
agents
cannot
guarantee
the
stability
activity
of
biocontrol.
Here,
18
synthetic
microbial
communities
different
scales
were
constructed,
with
best
effect
on
screened
through
in
vitro
vivo
experiments.
results
show
that
community
composed
Paenibacillus
amylolyticus,
Pseudomonas
putida,
Acinetobacter
calcoaceticus,
Serratia
proteamaculans,
Actinomycetia
bacterium
Bacillus
subtilis
has
activity.
Metabolomics
protoamaculans
interacts
other
member
strains
to
produce
caproic
acid
reduce
index
38.01%.
Furthermore,
mycelial
growth
inhibition
after
treatment
was
77.54%,
flow
cytometry
analysis
showed
living
conidia
rate
11.2%.
This
study
provides
value
for
application
potatoes,
as
well
interaction
mechanisms
between
communities.
