Direct and indirect effects of land use on microbiomes of trap-nesting solitary bee larvae and nests

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 8, 2025

The global decline in biodiversity and insect populations highlights the urgent need to conserve ecosystem functions, such as plant pollination by solitary bees. Human activities, particularly agricultural intensification, pose significant threats these essential services. Changes land use alter resource nest site availability, pesticide exposure other factors impacting richness, diversity, health of bee species. In this study, we investigated yet another facet currently less well context: Microbial communities associated with wild bees play crucial roles larval development, metabolism, immunity overall health. However, drivers dynamics healthy microbiome are still poorly understood, especially regarding direct indirect effects on diversity composition microbial communities. We examined bacterial offspring materials Megachilid trap-nesting bee, Osmia bicornis, along a gradient intensification 16S rRNA gene metabarcoding. Given that landscape composition, climatic conditions, food resources known influence compositions species, hypothesized changes would available for material collection thereby affecting microbiomes their environments. anticipated reduced altered increased which is decrease number resources, including pool floral soil bacteria surrounding environment. As expected, observed shifts nests across varying degrees intensity, differing management types availability flowers. Shannon (larval pollen provision, enclosure) guts decreased increasing intensity. pupae remained unaffected, indicating reorganization during metamorphosis, not significantly influenced resources. Our findings provide new insights into shaping environmental transmission microbiomes. This understanding comprehending impacts intensive developing strategies mitigate effects.

Language: Английский

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Bee Microbiomes: Unveiling Diversity and Social Dynamics in Managed and Wild Bees

Published: Jan. 1, 2025

Language: Английский

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Environmental and microhabitat influences on microbiota of snow-active Collembola in sub-zero temperatures

Applied Soil Ecology, Journal Year: 2025, Volume and Issue: 208, P. 105998 - 105998

Published: March 2, 2025

Language: Английский

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Social organization and physical environment shape the microbiome of harvester ants

Animal Microbiome, Journal Year: 2025, Volume and Issue: 7(1)

Published: March 19, 2025

All animals harbor microbiomes, which are obtained from the surrounding environment and impacted by host behavior life stage. To determine how two non-mutually exclusive drivers - physical social organization affect an organism's microbiome, we examined bacterial communities within around nests of harvester ants (Veromessor andrei). We collected soil nest content samples five different ant nests. used 16S rRNA gene sequencing calculated alpha beta diversity to compare community composition across samples. test hypotheses that and/or impact colonies' microbes compared our (i) sample types (ants, brood, seeds reproductives (winged alates), soil), (ii) inside outside nest, (iii) chamber types. Interestingly, found both microbiome V. andrei colonies. Soil differed one another in a way mapped onto their geographical distance. Furthermore, resembled soil, supporting hypothesis. However, associated with contents chambers, i.e., ants, seeds, reproductives, hypotheses. This study highlights importance considering environmental factors understanding dynamics.

Language: Английский

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Flowering Plant Microbiomes and Network Interactions Across an Urban Gradient

Environmental Microbiology, Journal Year: 2025, Volume and Issue: 27(4)

Published: March 28, 2025

ABSTRACT We used flowers to explore how ephemeral anthosphere microbiomes differ among flowering plant species and along an urban gradient. Here, we sequenced 16S rRNA for bacteria, ITS1 fungi rbcL DNA from 10 different sampled characterise gradient identify important network interactions. Bacterial fungal flower significantly differed in diversity across species, especially Asteraceae Fabaceae. Across all analyses, four taxa, the bacteria Pantoea Rosenbergiella Alternaria Cladosporium were highly prevalent contributed majority of microbiome composition differences observed between species. These taxa harbour strains or that may be either pathogenic beneficial plants. a land use gradient, community bacterial was stable consistent. Flower‐plant networks confirmed focal families abundance on each flower, with addition Paulowniaceae, suggesting pollinators visiting also visit this family. Our findings reveal are diverse at level encouragingly remain robust against urbanisation.

Language: Английский

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Standard methods and good practices in Apis honey bee omics research

Journal of Apicultural Research, Journal Year: 2025, Volume and Issue: 64(2), P. 307 - 402

Published: March 15, 2025

Language: Английский

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Environmental Effects on Bee Microbiota

Microbial Ecology, Journal Year: 2023, Volume and Issue: 86(3), P. 1487 - 1498

Published: April 26, 2023

Language: Английский

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Warming up through buildings and roads: what we know and should know about the urban heat island effect on bees

Frontiers in Bee Science, Journal Year: 2023, Volume and Issue: 1

Published: Nov. 28, 2023

Urbanization leads to cities having higher temperatures than surrounding non-urban areas [this is known as the urban heat island (UHI) effect]. Very little about impacts of UHI effect on bees, despite importance temperature many aspects bees’ life suggesting that these may be not negligible. In this study, we aimed highlight how could impact relevant functional traits bees in cities, proposing several ad hoc hypotheses for have thus far been investigated only few studies or at all, based what know from studies. The was shown influence bee body size, and generally tended reduce size cities. Urban also affect wing morphology, their overall flight morphology parameters. Individuals more brightly colored Bee ommatidial number antennal thermoreceptors they smaller fewer, respectively, areas. As expected, because face a risk desiccation, proportions alkanes longer main-carbon chain lengths are expected cuticular hydrocarbon (CHC) profiles. Stress biomarkers can occur greater concentrations specific bacteria gut lower abundances. Warm cycle pathogens by reducing proliferation. Aggression levels increased, eusocial species present worker phases per year due effect. All proposed likely visible solitary primitively species, which those suspected limited dispersal ability. Comparative would help proper testing hypotheses.

Language: Английский

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Integrative population genetics and metagenomics reveals urbanization increases pathogen loads and decreases connectivity in a wild bee

Global Change Biology, Journal Year: 2023, Volume and Issue: 29(15), P. 4193 - 4211

Published: May 12, 2023

As urbanization continues to increase, it is expected that two-thirds of the human population will reside in cities by 2050. Urbanization fragments and degrades natural landscapes, threatening wildlife including economically important species such as bees. In this study, we employ whole genome sequencing characterize genetics, metagenome microbiome, environmental stressors a common wild bee, Ceratina calcarata. Population genomic analyses revealed presence low genetic diversity elevated levels inbreeding. Through isolation distance, resistance, environment across urban found green spaces shrubs scrub were most optimal pathways for bee dispersal, conservation efforts should focus on preserving these land traits maintain high connectivity sites Metagenomic landscape exhibiting heat island effects, temperatures development but precipitation space, had highest taxa alpha all domains even when isolating potential pathogens. Notably, integration metagenomic data showed reduced areas not only correlated with lower relatedness among individuals also associated increased pathogen diversity, exposing vulnerable bees more Overall, our combined approach significant variation microbiomes nutritional resources absence differentiation, well enabled early detection health.

Language: Английский

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Wild bee and pollen microbiomes across an urban–rural divide

FEMS Microbiology Ecology, Journal Year: 2023, Volume and Issue: 99(12)

Published: Nov. 13, 2023

Abstract Wild pollinators and their microbiota are sensitive to land use changes from anthropogenic activities that disrupt landscape environmental features. As urbanization agriculture affect bee habitats, human-led disturbances driving in microbiomes, potentially leading dysbiosis detrimental fitness. This study examines the bacterial, fungal, plant compositions of small carpenter bee, Ceratina calcarata, its pollen provisions across an urban–rural divide. We performed metabarcoding C. calcarata Toronto by targeting 16S rRNA, ITS, rbcL regions. Despite similar composition diversity bees provisions, there was a greater microbial than bees. By characterizing differences use, climate, pesticide residues differentiate urban rural landscapes, we find areas support elevated levels more complex networks between microbes plants areas. However, may lead lower relative abundances known beneficial symbionts increased pathogens, such as Ascosphaera Alternaria fungi. Further, indicate dysregulate symbiosis. continue alter ever changing environments threaten crucial maintaining health.

Language: Английский

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