Bacterial communities of the threatened Western Pond Turtle may be impacted by land use

FEMS Microbiology Ecology, Journal Year: 2023, Volume and Issue: 99(12)

Published: Nov. 8, 2023

As semi-aquatic species that use both terrestrial and aquatic habitats, freshwater turtles their microbial communities are especially sensitive to the impacts of habitat disturbance. In this study, we 16S rRNA amplicon sequencing characterize shell cloacal bacterial in San Francisco Bay Area. We captured western pond (Actinemys/Emys marmorata) across eight sites located urban rural environments, along with invasive red-eared sliders (Trachemys scripta elegans). assessed differences turtle diversity/composition between samples evaluated how alpha/beta diversity metrics were influenced by quality. found phylum-level taxonomic turnover relative host tissue substrate samples. Our findings indicate location identity elicits a high degree lower-level (i.e. species/genus) turnover. Further, originating from good quality had poorer but more diverse ones. The overlapped those suggesting existence dispersal these two species. results add our current understanding symbiont ecology establishing patterns variation an gradient.

Language: Английский

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Microbiome and floral associations of a wild bee using biodiversity survey collections

Environmental Microbiology, Journal Year: 2024, Volume and Issue: 26(6)

Published: May 30, 2024

Abstract The health of bees can be assessed through their microbiome, which serves as a biomarker indicating the presence both beneficial and harmful microorganisms within bee community. This study presents characterisation bacterial, fungal, plant composition on cuticle adult bicoloured sweat ( Agapostemon virescens ). These were collected using various methods such pan traps, blue vane traps sweep netting across northern extent habitat range. Non‐destructive employed to extract DNA from whole pinned specimens these wild bees. Metabarcoding 16S rRNA, ITS rbcL regions was then performed. found that method collection influenced detection certain microbial taxa. Among methods, net samples showed lowest fungal alpha diversity. However, minor differences in bacterial or beta diversity suggest no single is significantly superior others. Therefore, combination techniques cater broader spectrum detection. also revealed regional variations core microbiome A. comprises two bacteria, three fungi association, all are commonly detected other microbes remained consistent different locations. Further extensive studies microbiomes species landscapes will help uncover crucial relationships between pollinator environment.

Language: Английский

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Direct and indirect effects of land use on microbiomes of trap-nesting solitary bee larvae and nests

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 28, 2024

The global decline in biodiversity and insect populations highlights the urgent to conserve ecosystem functions, such as plant pollination by solitary bees. Human activities, particularly agricultural intensification, pose significant threats these essential services. Changes land use alter resource nest site availability, pesticide exposure other factors impacting richness, diversity, health of bee species. In this study, we investigated yet another facet currently less well context: Microbial communities associated with wild bees play crucial roles larval development, metabolism, ­immunity overall health. However, drivers dynamics healthy microbiome are still poorly understood, especially regarding direct indirect effects on diversity composition microbial communities. We examined bacterial offspring materials Megachilid trap-nesting bee, O. bicornis,along a gradient intensification 16S rRNA gene metabarcoding. Given that landscape composition, climatic conditions, food resources known influence compositions species, hypothesized changes would available for material collection thereby affecting microbiomes their environments. anticipated reduced altered increased which is decrease number resources, including pool floral soil bacteria surrounding environment. As expected, observed shifts nests across varying degrees intensity, differing management types availability flowers. Shannon (larval pollen provision, enclosure) guts decreased increasing intensity. pupae remained unaffected, indicating reorganization during metamorphosis, not significantly influenced resources. Our findings provide new insights into shaping environmental transmission microbiomes. This understanding comprehending impacts intensive developing strategies mitigate effects.

Language: Английский

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Do local and landscape context affect the attractiveness of flower gardens to bees?

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(9), P. e0309000 - e0309000

Published: Sept. 4, 2024

Planting floral resources is a common strategy for increasing the abundance and diversity of beneficial flower-visiting insects in human-modified systems. However, context local area surrounding landscape may affect attractiveness these resource provisioning plots. We compared relative effects urban land-use on bees flowering plants gardens eastern Tennessee, USA. planted four types garden plots at each five different landscapes representing variety land use: 1) Urban Garden, 2) Forage Grassland, 3) Mixed Agriculture, 4) Forest, 5) Organic Farm. Each plot type had fixed plant community one three families (Asteraceae, Fabaceae, Lamiaceae) or mix all three, were replicated sites. concurrently sampled 50 m radius (local area) around found that size display (i.e. visual flowers) flowers did not bee species richness Although there was significant positive association between developed use 2 km gardens, effect small, no relationship area. There differences composition plots, but largest determinants variation bloom. This finding promising anyone wishing to promote pollinator populations by providing more resources.

Language: Английский

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The Gut Microbiome of Two Wild Bumble Bee Species Native of South America: Bombus pauloensis and Bombus bellicosus

Microbial Ecology, Journal Year: 2024, Volume and Issue: 87(1)

Published: Sept. 28, 2024

Language: Английский

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