Algae-fungi symbioses and bacteria-fungi co-exclusion drive tree species-specific differences in canopy bark microbiomes DOI Creative Commons
Jule Freudenthal, Kenneth Dumack,

Stefan Schaffer

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

With over 3 trillion trees, forest ecosystems comprise nearly one-third of the terrestrial surface Earth. Very little attention has been given to exploration above-ground plant microbiome its complex trophic interactions, and variations among tree species. To address this knowledge gap, we applied a primer-independent shotgun metatranscriptomic approach assess entire living canopy bark comprising prokaryotic eukaryotic primary producers, decomposers, various groups consumers. almost 1500 genera, found high microbial diversity on three species with distinct textures: oak (Quercus robur), linden (Tilia cordata), both rough bark, maple (Acer pseudoplatanus) smooth bark. Core co-occurrence network analysis revealed rich food web dominated by algal bacterial fungal sustaining diverse community consumers, including protists, microscopic metazoans, predatory bacteria. Whereas accommodated depauperate microbiome, richer mainly differing in their relative composition: Bacteria exhibited an increased dominance linden, whereas co-occurring algae fungi oak, highlighting importance algal-fungal lichen symbioses even at scale. Further, due bacteria-fungi co-exclusion, bacteria are not main beneficiaries algae-derived carbon compounds as it is known from aquatic systems.

Language: Английский

Phylogenetically distant but cohabiting: Fungal communities of fine roots in Diphasiastrum complanatum, Pinus sylvestris, and Vaccinium myrtillus in a Lithuanian pine forest DOI Creative Commons
Kristina Kuprina,

Moana Wirth,

Maria Luz Polo Luque

et al.

Rhizosphere, Journal Year: 2025, Volume and Issue: unknown, P. 101053 - 101053

Published: March 1, 2025

Language: Английский

Citations

1
Uneven-aged and even-aged forest management shape the soil fungal community composition in a boreal Norway spruce (Picea abies Karst) forest DOI Creative Commons
Eva-Maria Roth, Outi‐Maaria Sietiö, Sauli Valkonen

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 965, P. 178648 - 178648

Published: Feb. 1, 2025

Language: Английский

Citations

0
Fungal Community Dynamics in Cyperus rotundus: Implications for Rhizophora mangle in a Mangrove Ecosystem DOI Creative Commons
Diego Portalanza,

Arianna Acosta-Mejillones,

Johnny Alcívar

et al.

International Journal of Plant Biology, Journal Year: 2025, Volume and Issue: 16(1), P. 23 - 23

Published: Feb. 19, 2025

Mangrove ecosystems are globally significant for their biodiversity and ecosystem services but face persistent threats from invasive species anthropogenic disturbances. This study investigates the interactions between Cyperus rotundus, a widespread weed, fungal communities in mangrove-adjacent wetlands of Isla Santay, Ecuador. Using metagenomic sequencing ITS region, we analyzed diversity samples an anthropogenically pressured area non-impacted site. Results revealed differences microbial assemblages: rhizosphere sample disturbed exhibited lower richness was dominated by Magnaporthaceae (9%) Aureobasidium melanogenum (5%), both associated with stress-tolerant traits. In contrast, site showed higher diversity, Cladosporium dominicanum (62%) Talaromyces (11%) as dominant endophytic taxa. Principal Coordinates Analysis (PCoA) co-occurrence networks highlighted distinct partitioning two tissues, indicating that C. rotundus mediates composition response to environmental gradients. These findings underscore role plant’s success suggest leveraging beneficial fungi could enhance resilience support wetland restoration. By integrating molecular approaches ecological insights, this work contributes deeper understanding dynamics coastal informs targeted management strategies preserve mangrove habitats.

Language: Английский

Citations

0
Biotic interactions outweigh abiotic factors as drivers of bark microbial communities in Central European forests DOI Creative Commons
Lukas Dreyling, Caterina Penone, Noëlle V. Schenk

et al.

ISME Communications, Journal Year: 2024, Volume and Issue: 4(1)

Published: Jan. 1, 2024

Abstract Bark surfaces are extensive areas within forest ecosystems, which provide an ideal habitat for microbial communities, through their longevity and seasonal stability. Here we a comprehensive account of the bark surface microbiome living trees in Central European forests, identify drivers diversity community composition. We examine algal, fungal, bacterial communities interactions using metabarcoding on samples from over 750 collected Biodiversity Exploratories northern, central, southern Germany. show that mutual biotic influence is more important than abiotic environment with regard to composition, whereas conditions geography alpha diversity. Important factors relative humidity light availability, decrease algal but strongly increase fungal In addition, temperature shaping community, higher leading homogeneous dominant fungi, high turnover communities. Changes dissimilarity one organismal group occur close relation changes other two, suggesting there between three major groups may be linked beneficial exchange. To understand functioning as whole, need further investigate functionality combine these results findings habitats such soil or canopy.

Language: Английский

Citations

3
Surveying lichen diversity in forests: A comparison of expert mapping and eDNA metabarcoding of bark surfaces DOI Creative Commons
Lukas Dreyling, Steffen Boch, H. Thorsten Lumbsch

et al.

MycoKeys, Journal Year: 2024, Volume and Issue: 106, P. 153 - 172

Published: June 21, 2024

Lichens are an important part of forest ecosystems, contributing to biodiversity, the formation micro-niches and nutrient cycling. Assessing diversity lichenised fungi in complex such as forests, requires time substantial skills collecting identifying lichens. The completeness inventories thus largely depends on expertise collector, available for survey size studied area. Molecular methods surveying biodiversity hold promise overcome these challenges. DNA barcoding individual lichen specimens bulk collections is already being applied; however, eDNA have not yet been evaluated a tool surveys. Here, we assess which species can be detected swabbed from bark surfaces living trees central European forests. We compare our findings expert floristic carried out same plots about decade earlier. In total, 150 located three study regions across Germany. each plot, took one composite sample based six trees, belonging Fagus sylvatica , Picea abies Pinus sylvestris . method yielded 123 species, 87. total number found with both was 167, 48% were only eDNA, 26% methods. contained higher inconspicuous species. Many prevalent taxa reported could due gaps molecular reference databases. conclude that, currently, has merit complementary monitor at large scales, but cannot used its own. advocate further development specialised more complete

Language: Английский

Citations

2
Fungal Community Composition at the Last Remaining Wild Site of Yellow Early Marsh Orchid (Dactylorhiza incarnata ssp. ochroleuca) DOI Creative Commons

Andrea Dove,

Michael D. Charters, Matthew Campbell

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(8), P. 2124 - 2124

Published: Aug. 21, 2023

The yellow early marsh orchid (Dactylorhiza incarnata ssp. ochroleuca) is a critically endangered terrestrial in Britain. Previous attempts to translocate symbiotic seedlings site near the last remaining wild demonstrated some success, with 10% survival rate despite adverse weather conditions over two-year period. However, facilitate future reintroduction efforts or conservation translocations, more comprehensive understanding of fungal microbiome and abiotic soil characteristics at final required. Obtaining information on both community nutrient composition from sites has significant benefits may prove critical for success translocations involving threatened orchids. This preliminary study, conducted site, revealed correlation between relative abundance mycorrhizal order Cantharellales concentrations nitrate phosphate soil. Another group, Sebacinales, was found be distributed extensively throughout site. communities across entire orchid-hosting non-orchid-hosting soils discussed relation reinforcing current population preventing extinction this orchid.

Language: Английский

Citations

3
Ghost species form an important component of the epiphytic lichens in temperate forests DOI Creative Commons
Jan Vondrák, Jiří Košnar, Stanislav Svoboda

et al.

Forest Ecosystems, Journal Year: 2024, Volume and Issue: unknown, P. 100254 - 100254

Published: Sept. 1, 2024

Language: Английский

Citations

0
Algae-fungi symbioses and bacteria-fungi co-exclusion drive tree species-specific differences in canopy bark microbiomes DOI Creative Commons
Jule Freudenthal, Kenneth Dumack,

Stefan Schaffer

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

With over 3 trillion trees, forest ecosystems comprise nearly one-third of the terrestrial surface Earth. Very little attention has been given to exploration above-ground plant microbiome its complex trophic interactions, and variations among tree species. To address this knowledge gap, we applied a primer-independent shotgun metatranscriptomic approach assess entire living canopy bark comprising prokaryotic eukaryotic primary producers, decomposers, various groups consumers. almost 1500 genera, found high microbial diversity on three species with distinct textures: oak (Quercus robur), linden (Tilia cordata), both rough bark, maple (Acer pseudoplatanus) smooth bark. Core co-occurrence network analysis revealed rich food web dominated by algal bacterial fungal sustaining diverse community consumers, including protists, microscopic metazoans, predatory bacteria. Whereas accommodated depauperate microbiome, richer mainly differing in their relative composition: Bacteria exhibited an increased dominance linden, whereas co-occurring algae fungi oak, highlighting importance algal-fungal lichen symbioses even at scale. Further, due bacteria-fungi co-exclusion, bacteria are not main beneficiaries algae-derived carbon compounds as it is known from aquatic systems.

Language: Английский

Citations

0