Relationship between hindgut microbes and feed conversion ratio in Hu sheep and microbial longitudinal development DOI
Dan Xu, Jiangbo Cheng, Deyin Zhang

et al.

Journal of Animal Science, Journal Year: 2023, Volume and Issue: 101

Published: Jan. 1, 2023

Abstract Feed efficiency is an important indicator in the sheep production process, which plays role improving economic benefits and strengthening energy conservation emission reduction. Compared with rumen, fermentation of hindgut microorganisms can also provide part for host, composition will affect feed efficiency. Therefore, we hope to find new ways regulate by studying gut microbes. In this study, male Hu same birth date were raised under conditions until 180 d old. The divided into high low groups according conversion ratio (FCR) at 80 old, differences rectal between two compared. permutational multivariate analysis (PERMANOVA) test showed that there (P < 0.05). Combined linear fitting analysis, a total six biomarkers identified, including Ruminobacter, Eubacterium_xylanophilum_group, Romboutsia, etc. Functional enrichment may FCR through volatile fatty acids synthesis inflammatory response. At time, conducted longitudinal microbes, sampling nine-time points throughout market stages. microbiota clearly parts: before weaning after weaning, microbes are less affected microbial composition.

Language: Английский

Ecological and Functional Changes in the Hindgut Microbiome of Holstein Cows at High Altitudes DOI Creative Commons

Gong Chen,

Haibo Lu,

Shangzhen Huang

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(2), P. 218 - 218

Published: Jan. 15, 2025

The extreme environmental conditions of the Qinhai–Tibetan Plateau (QTP) challenge livestock survival and productivity, yet little is known about how high-altitude environments impact gut microbiota dairy cows. To fill this gap, we systematically investigated differences in hindgut microbiome between 87 plateau Holstein cows 72 plain using 16S rRNA gene sequencing. Our analysis revealed that group exhibited lower species richness but higher evenness than group. Additionally, significant separation composition two groups was observed based on altitude, while parity, days milk, age did not show a comparable impact. Moreover, altitude had lasting bacterial communities their co-occurrence networks, resulting reduced microbial interactions modularity Furthermore, identified four key taxa, Bacteroidaceae Rikenellaceae families, as well Prevotella Treponema genera, which were associated with regulation carbohydrate digestion energy metabolism might help adapt to environment. findings provide insights into strategies for enhancing adaptability through modulation, could ultimately contribute improving management sustainability these environments.

Language: Английский

Citations

0
Quantifying Greenhouse Gas Emissions from Livestock in Pastoral Areas based on Unmanned Aerial Vehicles DOI Creative Commons
Pengfei Liu, Min Liu,

Wanman Mei

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Abstract Accurate measurement of greenhouse gas (GHG) emissions from livestock is essential for developing effective emission reduction strategies. This study introduces the use Unmanned Aerial Vehicles (UAVs) and deep learning technology to estimate methane (CH₄) nitrous oxide (N₂O) enteric fermentation manure management in pastoral areas. More accurate animal GHG factors are derived by considering weight, feed quality, breeding methods, grassland types, which improves precision measuring livestock. Potential reductions different strategies, along with their associated costs benefits, projected identify optimal strategy. We also discuss appropriate carbon pricing mechanisms mitigating livestock-related emissions. These findings offer valuable guidance shaping strategies production.

Language: Английский

Citations

0
Identification of the key genes in Sanhe cattle for health and milk composition traits based on the WGCNA DOI Creative Commons
Ning Wang,

S.Q. Chen,

Yong Tang

et al.

Journal of Dairy Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Impact of high-altitude acclimatization and de-acclimatization on the intestinal microbiota of rats in a natural high-altitude environment DOI Creative Commons
Doudou Hao,

Haomeng Niu,

Qin Zhao

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: May 7, 2024

Intestinal microorganisms play an important role in the health of both humans and animals, with their composition being influenced by changes host's environment.

Language: Английский

Citations

1
Cow Milk Fatty Acid and Protein Composition in Different Breeds and Regions in China DOI Creative Commons

Yunxia Zou,

Yifei Chen, Qingyong Meng

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(21), P. 5142 - 5142

Published: Oct. 30, 2024

Cow milk is rich in proteins, fats, carbohydrates, and minerals; however, its precise nutrient content varies based on various factors. In the current study, we evaluated differences fatty acid protein contents of factors associated with these differences. To achieve this, samples were collected from seven types cows different regions. These included three dairy breeds: Chinese Holstein Beijing, China (BH), (HH) Jersey (JS) Hebei province, China; four dairy/meat Sanhe (SH) Inner Mongolia Autonomous Region, China, Xinjiang brown (XH) Simmental (SI) Uygur Shu Xuanhua (SX) Sichuan China. Breed significantly affects total fat, acid, contents. Additionally, geographic region acids, α-lactalbumin, lactoferrin. JS has highest fat casein XH contain higher unsaturated than BH do not differ JS. low β-lactoglobulin high lactoferrin may be favorable for growth development infants. Our results inform products cow breeds advance process accurate breed identification.

Language: Английский

Citations

1
Drinking Warm Water Promotes Performance by Regulating Ruminal Microbial Composition and Serum Metabolites in Yak Calves DOI Creative Commons
Tianxu Liu,

Qianqian Wang,

Chenxi Gao

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(8), P. 2092 - 2092

Published: Aug. 16, 2023

Yaks live in the harsh environment of Qinghai-Tibet Plateau, and cold climate causes lower growth efficiency. The aim this experiment was to explore effects drinking warm water on performance yak calves investigate underlying physiological mechanisms. A total 24 Datong were selected randomly assigned into group (group C, temperature around 0-10 °C without any heating; 58.03 ± 3.111 kg) W, constantly heated at 2 °C; 59.62 2.771 kg). After 60-day experiment, body weight measured, rumen fluid blood serum samples collected for analysis. results show that average daily gain yaks drank higher compared those (p < 0.05). acetic, propionic, isobutyric, valeric, isovaleric acid concentrations W than C Additionally, changed ruminal microbes different levels. At phylum level, relative abundance Tenericutes, Kiritimatiellaeota, Elusimicrobiota genus three genera increased by water, including Ruminococcoides Eubacteriales Family XIII. Incertae Sedis, 12 decreased, Ruminococcus species unclassified Prevotellaceae bili According metabolomics results, metabolites, valine, isoleucine, PC (15:0/22:2(13Z,16Z)), LysoPC (18:0/0:0), 0.05), enriched glycerophospholipid amino metabolism pathways. This study analyzed differences metabolomes provided with temperatures revealed potential mechanism better promoted water.

Language: Английский

Citations

3
How hypoxia affects microbiota metabolism in mice DOI Creative Commons

Ainiwaer Ailizire,

Xiaojing Wang, Yan Ma

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 28, 2023

To investigate the relationship between gut microbiota and fecal metabolites of hypoxic environments in mice.High-fat diet-induced obese mice (n = 20) normal diet-fed were randomly divided into four groups: high altitude group (HOB), weight (HN), low LOB (LOB), (LN). Fecal samples from each 16S rRNA gene sequenced, five groups above selected for non-targeted metabolomics analysis using liquid chromatography-mass spectrometry. The was analyzed SIMCA 14.1, MetaboAnalyst 5.0 R 4.1.11.(A) Body significantly lower obesity than normoxic group. (B) Differences α-diversity β-diversity found different body weights altitude, diversity higher group; results comparison two showed that Faecalibaculum, Romboutsia, Lactobacillus, A2 associated with obesity; Romboutsia hypoxia. (C) metabolic profiles differed nucleotide amino acid metabolism same groups, while lipid oxygen concentration groups.(a) Gut reduced groups. dominant hypoxia (b) they

Language: Английский

Citations

3
Gut Microbiota Influence Severity Of High Altitude Pulmonary Edema and Reveal Pathogenesis DOI

Xianduo Sun,

Gaosheng Hu, Yuting Li

et al.

Published: Jan. 1, 2024

Background: High altitude pulmonary edema (HAPE) is a life-threatening illness. However, the link between gut microbiota and HAPE, differences in specific microbial species that influence mechanisms by which they might work, remain unknown. Methods: We recruited 15 low-altitude high-altitude volunteers used Lake Louise Acute Mountain Sickness (AMS) score to estimate AMS. Gut were profiled using metagenomic sequencing of fecal samples. Fecal transplantation, lung water content histological analysis determine role HAPE. Transcriptome, lipidomics cytokine investigate potential mechanism biomarkers target bacteria promoting The detailed underlying was investigated cell experiments molecular dynamics simulation.Findings: This study found intestinal Klebsiella pneumoniae (K. pneumoniae) Escherichia coli (E. coli) are main pathogens cause HAPE inducing inflammation, disrupting lipid metabolism elevating lysophosphatidylcholines (LPCs) levels. Conversely, synthetic containing Bifidobacterium, Lactiplantibacillus, fructooligosaccharides isomaltose-oligosaccharides significantly reduced severity Further investigation revealed LPCs can damage permeability human microvascular endothelial alveolar epithelial monolayers under hypoxic conditions inserting aggregating into membrane integrity.Interpretation: describes microbiota-LPCs/inflammation-HAPE axis, an important insight will help open avenues for goal-directed prevention treatment approaches.Funding: work supported Foundation Shenyang Pharmaceutical University (52104003 51110656).Declaration Interest: authors declare no competing interest.Ethical Approval: All research procedures involving subjects approved Ethics Committee Maqin County People's Hospital, Golog Prefecture, Qinghai Province (Study No. MQXrmyyll-2019-001). Written informed consent obtained from all volunteers. conducted following Guideline Animal Experimentation institution (approval number: SYPU-IACUC-2019-1227-205).

Language: Английский

Citations

0
Survey of the fecal microbiota of indigenous small ruminants living in different areas of Guizhou DOI Creative Commons
Wei Guo,

Tingmei Liu,

Weiwei Wang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 8, 2024

Gut microbiota are associated with the health and performance of ruminant species, they affected by altitude, host genetics, sex. However, there has been little research on comparing fecal indigenous small ruminants such as sheep goats in Guizhou province, China. In present study, we revealed effect sex profiles enterotypes Fecal samples were collected from Hei Qianbei Ma Weining Chinese province Guizhou. 16S rRNA gene sequencing targeting V3-V4 region was performed using Illumina MiSeq platform. Sequences processed QIIME2, qualified sequences plugin DADA2 to generate amplicon sequence variants (ASVs). The statistical analysis R studio. microbial profile found vary herd (influenced genetics/altitude) All categorized into two enterotypes. first enterotype is dominated UCG-005, second Christensenellaceae_R-7_group, which may be highly driven host's genetics (breed). predicted functional also assigned clusters that corresponded exactly Cluster 1 profiling characterized biosynthesis pathways, cluster 2 energy metabolism pathways. Our findings provide new insights community herds, offering clues for understanding mechanisms contribute divergent phenotypes

Language: Английский

Citations

0
Differences in Gut Microbes Across Age and Sex Linked to Metabolism and Microbial Stability in a Hibernating Mammal DOI Creative Commons

Madison Pfau,

Samuel Degregori, Paul H. Barber

et al.

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(11)

Published: Nov. 1, 2024

ABSTRACT The gut microbiome has a well‐documented relationship with host fitness, physiology, and behavior. However, most of what is known comes from captive animals where diets environments are more homogeneous or controlled. Studies in wild populations that experience dynamic have natural life history variation less common but key to understanding the drivers microbiome. Here we examine population yellow‐bellied marmots ( Marmota flaviventer ), an obligate winter hibernator, quantify multivariate associations between host‐associated factors (e.g., age, sex, environmental harshness, social behavior) microbial composition. Across 5 years 143 individuals, found males had higher relative abundance microbes associated mass gain cellulose digestion, which suggests metabolic investment (such as phylum Firmicutes family Lachnospiraceae ). By contrast, females abundances inflammation metabolism (from groups such Tenericutes Ruminococcus possibly reflecting importance lactation offspring investment. Post hoc analyses lactating showed negative positive energy, suggesting trade‐off pups maternal gain. Older also reduced Proteobacteria abundance, inflammation. Results demonstrate sex age‐based traits, not sociality microbe‐mediated wild, hibernating mammal.

Language: Английский

Citations

0