Impact of Sample Storage Time and Temperature on the Stability of Respiratory Viruses and Enteric Viruses in Wastewater DOI Creative Commons
Yuanyuan Qiu,

Richardson Mah,

Logan A. Brand

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2459 - 2459

Published: Nov. 29, 2024

Wastewater-based surveillance (WBS) has been widely used to track SARS-CoV-2 as well many other viruses in communities during the COVID pandemic and post-pandemic. However, it is still not clear how temperature storage time would influence stability of wastewater. In this study, we assessed SARS-CoV-2, pepper mild mottle virus (PMMoV), influenza A (IAV) B (IBV), respiratory syncytial (RSV), enteric raw wastewater stored at room temperature, 4 °C, −20 °C for 3 6 days. PMMoV, IAV, were found be stable up days after storing or °C. RSV more susceptible freeze–thaw cycles compared PMMoV viruses, which relatively Low detection IBV made difficult evaluate impact. Based on our findings, conclude that short-term transportation samples within ambient acceptable majority these viruses. Freezing even short periods recommended WBS The data obtained from study can provide guidance quality assurance purposes operational aspects surveillance.

Language: Английский

Evaluation of plasmid pBI143 for its optimal concentration methods, seasonal impact, and potential as a normalization parameter in wastewater-based epidemiology DOI Creative Commons
Bikash Malla, Sadhana Shrestha, Niva Sthapit

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 965, P. 178661 - 178661

Published: Feb. 1, 2025

Language: Английский

Citations

3
Beyond COVID-19: Wastewater-based epidemiology for multipathogen surveillance and normalization strategies DOI Creative Commons
Bikash Malla, Sadhana Shrestha, Niva Sthapit

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 946, P. 174419 - 174419

Published: July 2, 2024

Wastewater-based epidemiology (WBE) is a critical tool for monitoring community health. Although much attention has focused on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causative agent of disease 2019 (COVID-19), other pathogens also pose significant health risks. This study quantified the presence SARS-CoV-2, influenza A virus (Inf-A), and noroviruses genogroups I (NoV-GI) II (NoV-GII) in wastewater samples collected weekly (n = 170) from July 2023 to February 2024 five treatment plants (WWTPs) Yamanashi Prefecture, Japan, by quantitative PCR. Inf-A RNA exhibited localized prevalence with positive ratios 59 %–82 % different WWTPs, suggesting regional outbreaks within specific areas. NoV-GI (94 %, 160/170) NoV-GII (100 170/170) were highly prevalent, (6.1 ± 0.8 log10 copies/L) consistently exceeding (5.4 0.7 concentrations. SARS-CoV-2 was detected 100 samples, mean concentrations 5.3 0.5 copies/L WWTP E 5.8 0.4 each WWTPs. Seasonal variability evident, higher all pathogenic viruses during winter. Non-normalized normalized fecal indicator bacteria (Escherichia coli total coliforms), an (pepper mild mottle (PMMoV)), turbidity revealed associations reported cases. + GII showed strong correlations gastroenteritis cases, particularly when E. (Spearman's ρ 0.70–0.81) coliforms (ρ 0.70–0.81), respectively. For non-normalized correlation 0.61, decreasing 0.31 PMMoV, that PMMoV unsuitable. Turbidity normalization yielded suboptimal results. underscored importance selecting suitable parameters tailored accurate trend using WBE, demonstrating its utility beyond COVID-19 surveillance.

Language: Английский

Citations

6
Comparing the decay of human wastewater-associated markers and enteric viruses in laboratory microcosms simulating estuarine waters in a temperate climatic zone using qPCR/RT-qPCR assays DOI Creative Commons
Warish Ahmed,

Asja Korajkic,

Wendy Smith

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 908, P. 167845 - 167845

Published: Nov. 16, 2023

This study investigated the decay rates of wastewater-associated markers and enteric viruses in laboratory microcosms mimicking estuarine water environments temperate Sydney, NSW, Australia using qPCR RT-qPCR assays. The results demonstrated reduction concentrations Bacteroides HF183, Lachnospiraceae Lachno3, cross-assembly phage (crAssphage), pepper mild mottle virus (PMMoV), human adenovirus (HAdV 40/41), enterovirus (EV) over a span 42 days under spring/summer temperatures, presence/absence microbiota, different light conditions. found that crAssphage, PMMoV, HAdV 40/41, EV exhibited varying depending on experimental average T

Language: Английский

Citations

9
The influence of environmental factors on the detection and quantification of SARS-CoV-2 variants in dormitory wastewater at a primarily undergraduate institution DOI Creative Commons
Chequita N. Brooks,

Suzanne Brooks,

Jeannette M. Beasley

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

ABSTRACT Testing for the causative agent of coronavirus disease 2019 (COVID-19), severe acute respiratory syndrome 2 (SARS-CoV-2), has been crucial in tracking spread and informing public health decisions. Wastewater-based epidemiology helped to alleviate some strain testing through broader, population-level surveillance, applied widely on college campuses. However, questions remain about impact various sampling methods, target types, environmental factors, infrastructure variables SARS-CoV-2 detection. Here, we present a data set over 800 wastewater samples that sheds light influence variety these factors quantification using droplet digital PCR (ddPCR) from building-specific sewage infrastructure. We consistently quantified significantly higher number copies virus per liter nucleocapsid (N2) compared 1 (N1), regardless method (grab vs composite). further show dormitory-specific differences abundance, including correlations dormitory population size. Environmental like precipitation temperature little no load, with exception temperatures grab sample data. observed gene copy numbers Omicron variant than Delta within ductile iron pipes but difference abundance (N1 or N2) across three different pipe types our set. Our results indicate contextual should be considered when interpreting wastewater-based epidemiological IMPORTANCE viral RNA is shed by symptomatic asymptomatic infected individuals, allowing its genetic material detected wastewater. used measure several dormitories Appalachian State University campus examined quantification. Changes were based type, as well trends variants method. These highlight value applying data-inquiry practices this study better contextualize results.

Language: Английский

Citations

0
Wastewater-based epidemiology of influenza viruses: a systematic review DOI Creative Commons
Luca Viviani, Riccardo Vecchio, Elena Pariani

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 986, P. 179706 - 179706

Published: May 30, 2025

Language: Английский

Citations

0
SARS-CoV-2 virus in raw wastewater from student residence halls with concomitant 16S rRNA bacterial community structure changes DOI Creative Commons
Ye Li, Kurt T. Ash, Dominique C. Joyner

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: June 2, 2025

The detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in sewage is well-established, but the concomitant changes microbial compositions during pandemic remain insufficiently explored. This study investigates impact SARS-CoV-2 virus on raw sewage, utilizing high-throughput 16S rRNA amplicon sequencing to analyze wastewater samples collected from six dormitories over a one-year field trial at University Tennessee, Knoxville. concentration was assessed using reverse transcription-quantitative polymerase chain reaction. Significant variations bacterial composition were evident across dormitories, highlighting importance independently considering spatial differences when evaluating microbiome. Positive for exhibited prominent representation exclusive species all coupled with significantly reduced diversity compared negative samples. correlation observed between relative abundance enteric pathogens and potential sampling sites introduces significant dimension our understanding COVID-19, especially notable positive Furthermore, may be linked enduring effects dysbiosis COVID-19 recovery. These findings provide valuable insights into dynamics pandemic.

Language: Английский

Citations

0
Evaluating various composite sampling modes for detecting pathogenic SARS-CoV-2 virus in raw sewage DOI Creative Commons
Li Ye, Kurt T. Ash, Dominique C. Joyner

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 23, 2023

Inadequate sampling approaches to wastewater analyses can introduce biases, leading inaccurate results such as false negatives and significant over- or underestimation of average daily viral concentrations, due the sporadic nature input. To address this challenge, we conducted a field trial within University Tennessee residence halls, employing different composite modes that encompassed time intervals (1 h, 2 4 6 24 h) across various windows (morning, afternoon, evening, late-night). Our primary objective was identify optimal approach for generating representative samples SARS-CoV-2 from raw wastewater. Utilizing reverse transcription-quantitative polymerase chain reaction, quantified levels RNA pepper mild mottle virus (PMMoV) in sewage. findings consistently demonstrated PMMoV RNA, an indicator human fecal contamination water environment, exhibited higher abundance lower variability compared pathogenic RNA. Significantly, both greater 1 h individual throughout entire period, contrasting with stability observed other time-based samples. Through comprehensive analysis using Quade Nonparametric ANCOVA test date, concentration site covariates, concluded sampler during focused morning window is pragmatic cost-effective strategy achieving single day wastewater-based epidemiology applications. This method has potential significantly enhance accuracy reliability data collected at community level, thereby contributing more informed public health decision-making pandemic.

Language: Английский

Citations

8
Cold-chain free nucleic acid preservation using porous super-absorbent polymer (PSAP) beads to facilitate wastewater surveillance DOI
Gyuhyon Cha, Yixuan Huang, Katherine E. Graham

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 939, P. 173468 - 173468

Published: May 22, 2024

Language: Английский

Citations

2
Detection of SARS-CoV-2 in Wastewater Associated with Scientific Stations in Antarctica and Possible Risk for Wildlife DOI Open Access
Marcelo González‐Aravena, Cristobal Galbán‐Malagón, Eduardo Castro‐Nallar

et al.

Published: Feb. 5, 2024

Before December 2020, Antarctica had remained free of Covid-19 cases. The main concern during the pandemic was limited health facilities available at Antarctic stations to deal with disease, as well potential impact SARS-CoV-2 on wildlife through reverse zoonosis. In 60 cases emerged in Chilean stations, disrupting summer campaign ongoing isolation needs. RNA detected wastewater several scientific stations. Antarctica, treated is discharged directly into seawater. No studies currently address recovery infectious virus particles from wastewater, but their presence raises risk infecting and initiating new replication cycles. This study highlights initial detection identifying viral via RT-qPCR targeting various genomic regions. virus's found effluent two plants Maxwell Bay O’Higgins Station King George Island Peninsula, respectively. explores for zoonotic transmission humans due direct release implications such underscore need continued vigilance research.

Language: Английский

Citations

1
SARS-CoV-2 virus in Raw Wastewater from Student Residence Halls with concomitant 16S rRNA Bacterial Community Structure changes DOI Open Access
Ye Li, Kurt T. Ash, Dominique C. Joyner

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 13, 2024

Abstract The detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in sewage is well-established, but the concomitant changes microbial compositions during pandemic remain insufficiently explored. This study investigates impact SARS-CoV-2 virus on raw sewage, utilizing 16S rRNA sequencing to analyze wastewater samples collected from six dormitories over a one-year field trial at University Tennessee, Knoxville. concentration was assessed using reverse transcription-quantitative polymerase chain reaction. Significant variations bacterial composition were evident across dormitories, highlighting importance independently considering spatial differences when evaluating microbiome. Positive for exhibited prominent representation exclusive species all coupled with significantly reduced diversity compared negative samples. correlation observed between relative abundance enteric pathogens and potential sampling sites introduces significant dimension our understanding COVID-19, especially notable positive Furthermore, may be linked enduring effects dysbiosis COVID-19 recovery. These findings provide valuable insights into dynamics pandemic.

Language: Английский

Citations

1