Two new psychrotolerant Massilia species inhibit plant pathogens Clavibacter and Curtobacterium DOI Creative Commons
Ivo Sedláček, Pavla Holochová, Karel Sedlář

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 13, 2024

Abstract Three bacterial strains producing blue-violet pigmented colonies on R2A agar were isolated from a wet rock wall and lakes in deglaciated northern part of James Ross Island, Antarctica. The inhibited phytopathogenic Gram-positive bacteria, e.g. Clavibacter spp., Curtobacterium flacumfaciens, Paenarthrobacter ilicis. Phylogenetic analysis based the 16S rRNA gene indicated that isolates belonged to genus Massilia closest relatives violaceinigra B2T, rubra CCM 8692T, frigida 8695T, antarctica 8941T, aquatica 8693T. A polyphasic taxonomic study lepA genes sequencing, automated ribotyping, MALDI-TOF MS, chemotaxonomy analyses, extensive biotyping, average nucleotide identity, digital DNA-DNA hybridization calculations whole-genome sequences proved represent novel species for which names pseudviolaceinigra sp. nov. scottii are suggested, with type P3689T (= 9206T) P5043T 9029T), respectively. These two bioactive-substances-producing may play an important role shaping composition fresh-water Antarctic microbiomes due inhibition various bacteria.

Language: Английский

Prodigiosin: unveiling the crimson wonder – a comprehensive journey from diverse bioactivity to synthesis and yield enhancement DOI Creative Commons

Yonglin Lu,

D Liu,

Renhui Jiang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: June 5, 2024

Prodigiosin (PG) is a red tripyrrole pigment from the prodiginine family that has attracted widespread attention due to its excellent biological activities, including anticancer, antibacterial and anti-algal activities. The synthesis production of PG particular significance, as it potential be utilized in number applications, those pertaining clinical drug development, food safety, environmental management. This paper provides systematic review recent research on PG, covering aspects like chemical structure, bioactivity, biosynthesis, gene composition regulation, optimization conditions, with focus biosynthesis regulation Serratia marcescens. solid theoretical basis for development expected promote further medicine other applications.

Language: Английский

Citations

10
Antarctic bacterial natural products: from genomic insights to drug discovery DOI Creative Commons
William Bartolomeu Medeiros, Stanislava Králová,

Valéria Oliveira

et al.

Natural Product Reports, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The article highlights the available data on Antarctic bacteria, their biosynthetic potential, and successful NP discoveries while addressing challenges in research advocating for systematic, collaborative Antarctica.

Language: Английский

Citations

0
Bacterial diversity and geomicrobiology of Winter Wonderland ice cave, Utah, USA DOI Creative Commons

Miranda Seixas,

Jeffrey S. Munroe, Erin M. Eggleston

et al.

MicrobiologyOpen, Journal Year: 2024, Volume and Issue: 13(4)

Published: July 12, 2024

The Winter Wonderland ice cave, located at an elevation of 3140 m above sea level in the Uinta Mountains northern Utah, USA, maintains a constant sub-zero temperature. Seasonal snowmelt and rain enter freeze on surface existing ice, contribute to 3-m-thick layered mass. This mass contains organic matter cryogenic cave carbonates (CCCs) that date back centuries. In this study, samples liquid water, exposed CCCs were collected examine bacterial communities within determine if these vary spatially between sample types. Flow cytometry showed cell counts are order magnitude higher water than ice. Epifluorescence microscopy scanning electron imaging revealed potential coccoid bacillus microbial morphologies putative cells or calcite spherules CCCs. diversity bacteria associated with soil, identified through sequence-based analysis, supports hypothesis enters by filtering soil bedrock. A differential abundance taxa was observed types, greatest found geomicrobiological framework where microbes aggregate sink into concentrated layer, precipitate out CCCs, thereby reducing These may provide essential nutrients for could themselves be products biomineralization.

Language: Английский

Citations

1
Toward an integrated understanding of how extreme polar light regimes, hybridization, and light-sensitive microbes shape global biodiversity DOI Creative Commons
Kari Saikkonen, Traci Birge, Benjamin Fuchs

et al.

One Earth, Journal Year: 2024, Volume and Issue: 7(9), P. 1529 - 1541

Published: Sept. 1, 2024

Language: Английский

Citations

1
Effects of mulch films with different thicknesses on the microbial community of tobacco rhizosphere soil in Yunnan laterite DOI Creative Commons
Shuaibing Wang, Qiuping Li,

Changbing Ye

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 23, 2024

The mulch film (MF) management model of the agricultural field affects physical and chemical properties soil (PCPS) structure microorganism community; however, studies on relationship between rhizosphere community thickness MF are still limited. To understand interactions among thickness, PCPS, microorganism, a study was conducted by using an integrated metagenomic strategy, where tobacco treated with four commonly representative used thicknesses MFs (0.004, 0.006, 0.008, 0.010 mm) in Yunnan laterite. results showed that agronomic traits such as plant height (TPH), leaf number (LN), fresh weight (FLW), dry (DLW) were significantly ( p < 0.01) improved mulched thickest (0.010 compared exposed (CK), there 6.81 5.54% increase FLW TPH, separately. correlation analyses revealed significant positive water content (SWC), organic matter (SOM), total nitrogen (TN), available (AN), phosphorus (TP), (AP; all 0.01), while negatively correlated temperature (ST; 0.01). In addition, bacteria changed overall which also interfered function bacteria. abundance Bradyrhizobium Nitrospira positively Sphinsinomonas Massilia it. This indicated ability to utilize N remove harmful molecules strengthened, capacity degrade pollutants greatly reduced. These findings provide additional insights into potential risks application different MFs, particularly concerning PCPS microbial communities.

Language: Английский

Citations

1
OpenASAP: An affordable 3D printed atmospheric solids analysis probe (ASAP) mass spectrometry system for direct analysis of solid and liquid samples DOI Creative Commons
Robert M. Samples,

Riko Mukoyama,

Jacob M. C. Shaffer

et al.

HardwareX, Journal Year: 2023, Volume and Issue: 16, P. e00490 - e00490

Published: Nov. 17, 2023

Atmospheric Solids Analysis Probe (ASAP) mass spectrometry is a versatile technique allowing direct sampling of solid and liquid samples, but its adoption limited due to the high cost commercial ASAP systems. To address this, we present OpenASAP, an open-source system for spectrometers that can be fabricated $20 or less using 3D-printing. Our design readily adaptable instruments from different manufacturers produced with variety additive manufacturing techniques on consumer-grade 3D-printers. The probe allows rapid samples without sample preparation, making it useful throughput screening, investigating spatial localization function analytes in biological incorporating instructional settings. We demonstrate effectiveness by obtaining spectra three natural product standards at levels as low 10 ng/ml detecting these metabolites microbial cultures are difficult analyze complex matrices analyte properties. Furthermore, thin layer chromatography (TLC) spots cultures.

Language: Английский

Citations

2
Molecular Networking‐Guided Discovery of Kyonggic Acids in Massilia spp. DOI Creative Commons
Till Steinmetz,

Anton Lindig,

Stephan Lütz

et al.

European Journal of Organic Chemistry, Journal Year: 2024, Volume and Issue: 27(17)

Published: March 21, 2024

Abstract In recent years, bacteria of the genus Massilia have been identified as promising sources bioactive natural products. this study, we used mass spectrometry (MS)‐based molecular networking for first comprehensive analysis secondary metabolome these microorganisms. Annotation detected metabolite clusters with a fragmentation database revealed several compounds not previously known to be produced by species. To secure sufficient material further structural analyses well biological testing, selected most producer strains from our and subjected them larger‐scale cultivation. Following extraction UV‐directed isolation, obtained five metabolites in milligram quantities. addition cyclic dipeptide, elucidated structures four acyl amino acids that are related thalassotalic acid C marine bacterium Thalassotalea sp. The ‐derived kyonggic were found inhibit enzyme tyrosinase, which is involved melanin biosynthesis influencing skin hair pigmentation. This study illustrates value combining spectroscopic computational tools product discovery order detect guide isolation overlooked compounds.

Language: Английский

Citations

0
Two new psychrotolerant Massilia species inhibit plant pathogens Clavibacter and Curtobacterium DOI Creative Commons
Ivo Sedláček, Pavla Holochová, Karel Sedlář

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 13, 2024

Abstract Three bacterial strains producing blue-violet pigmented colonies on R2A agar were isolated from a wet rock wall and lakes in deglaciated northern part of James Ross Island, Antarctica. The inhibited phytopathogenic Gram-positive bacteria, e.g. Clavibacter spp., Curtobacterium flacumfaciens, Paenarthrobacter ilicis. Phylogenetic analysis based the 16S rRNA gene indicated that isolates belonged to genus Massilia closest relatives violaceinigra B2T, rubra CCM 8692T, frigida 8695T, antarctica 8941T, aquatica 8693T. A polyphasic taxonomic study lepA genes sequencing, automated ribotyping, MALDI-TOF MS, chemotaxonomy analyses, extensive biotyping, average nucleotide identity, digital DNA-DNA hybridization calculations whole-genome sequences proved represent novel species for which names pseudviolaceinigra sp. nov. scottii are suggested, with type P3689T (= 9206T) P5043T 9029T), respectively. These two bioactive-substances-producing may play an important role shaping composition fresh-water Antarctic microbiomes due inhibition various bacteria.

Language: Английский

Citations

0