Biofilm,
Journal Year:
2024,
Volume and Issue:
7, P. 100195 - 100195
Published: April 8, 2024
Interspecies
interactions
within
a
biofilm
community
influence
population
dynamics
and
structure,
which
in
turn
may
affect
the
bacterial
stress
response
to
antimicrobials.
This
study
was
conducted
assess
impact
of
between
Applied Sciences,
Journal Year:
2024,
Volume and Issue:
14(18), P. 8137 - 8137
Published: Sept. 10, 2024
Biofilm-associated
infections
present
a
significant
challenge
in
modern
medicine,
primarily
due
to
their
resilience
and
resistance
conventional
treatments.
These
occur
when
bacteria
form
biofilms,
protective
layers
formed
by
bacterial
communities,
which
are
notoriously
resistant
traditional
antibiotics
on
surfaces
such
as
medical
implants
biological
surfaces,
making
eradication
with
standard
difficult.
This
leads
persistent
infections,
imposing
substantial
economic
burden
healthcare
systems.
The
urgency
find
alternative
treatments
is
critical
current
methods
insufficient
costly.
Innovative
approaches,
nanotechnology-based
therapies,
offer
promising
alternatives
targeting
biofilms
more
effectively
reducing
the
need
for
invasive
procedures.
Nanocarriers
hold
promise
fight
against
biofilm-associated
infections.
can
penetrate
than
treatments,
delivering
higher
concentrations
of
or
other
antimicrobial
agents
precisely
where
they
needed.
targeted
approach
not
only
enhances
efficacy
but
also
minimizes
potential
side
effects.
development
nanocarrier-based
therapies
crucial
overcoming
limitations
ultimately
improving
patient
outcomes
In
this
review,
systems,
characteristics,
limitations,
benefits
explored
address
biofilms-related
Additionally,
biofilm
evaluation
models
tests
necessary
preclinical
validation
these
nanosystems
facilitate
clinical
application
addressed.
ACS Environmental Au,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 22, 2025
Many
polycyclic
aromatic
hydrocarbons
(PAHs)
in
the
environment
resulting
from
crude
oil
spills
and
incomplete
combustion
of
organic
matter
are
highly
toxic,
mutagenic,
or
carcinogenic
to
microorganisms
humans.
Bioremediation
PAHs
using
that
encode
biodegradative
genes
is
a
promising
approach
for
environmental
PAH
cleanup.
However,
viability
exogenous
often
limited
due
competition
with
native
microbial
community.
Instead
relying
on
survival
one
few
species
bacteria,
genetic
bioaugmentation
harnesses
conjugative
plasmids
spread
functional
microbes.
In
this
study,
two
plasmid
backbones
differ
copy
number
regulation,
replication,
mobilization
were
engineered
contain
dioxygenase
gene
(bphC)
conjugated
soil
bacteria
including
Bacillus
subtilis,
Pseudomonas
putida,
Acinetobacter
sp.,
as
well
synthetic
community
assembled
these
bacteria.
Fitness
effects
transconjugants
significantly
impacted
rates
transfer
biotransformation
model
(2,3-dihydroxybiphenyl).
A
synergistic
effect
was
observed
which
communities
bioaugmented
bphC
had
higher
degradation
than
grown
monocultures.
Finally,
conjugation
associated
relative
abundances
communities,
underscoring
how
fitness
impacts
can
shape
structure
function.
Foods,
Journal Year:
2025,
Volume and Issue:
14(9), P. 1517 - 1517
Published: April 26, 2025
Next-Generation
Sequencing
(NGS)
technology
was
applied
to
evaluate
Food
Safety
Management
System
(FSMS)
performance
in
seafood-processing
factories
by
exploring
microbiome
diversity
alongside
traditional
methods
for
detecting
foodborne
pathogens.
A
total
of
210
environmental
swabs
collected
from
processing
zones
six
underwent
16S
rRNA
amplicon
sequencing.
FSMS-certified
exhibited
significantly
higher
species
richness,
with
alpha
p-values
0.0036
observed
ASVs,
0.0026
Faith’s
PD
and
0.032
Shannon.
Beta
analysis
also
revealed
significant
differences,
0.001
Bray–Curtis,
unweighted
UniFrac
Jaccard.
Pathogens
like
Listeria
monocytogenes,
Salmonella
spp.
Bacillus
cereus
were
present
“uncertified”
but
absent
the
“certified”
factories.
The
had
a
proportion
lactic
acid
bacteria
(LAB)
genera
(70.22%)
compared
(29.78%).
LAB
included
Streptococcus,
Lactococcus,
Lactobacillus
others.
NGS
has
demonstrated
superior
capability
providing
comprehensive
detection,
including
unculturable
microorganisms
insights
into
microbial
diversity,
so
it
lacks
limitations
that
come
culturing.
These
findings
highlight
potential
leveraging
beneficial
microbes
bioremediation
pathogen
control
enhance
FSMS
effectiveness
environments.
ISME Communications,
Journal Year:
2023,
Volume and Issue:
3(1)
Published: Nov. 15, 2023
Abstract
Identifying
interspecies
interactions
in
mixed-species
biofilms
is
a
key
challenge
microbial
ecology
and
of
paramount
importance
given
that
govern
community
functionality
stability.
We
previously
reported
bacterial
four-species
biofilm
model
comprising
Stenotrophomonas
rhizophila,
Bacillus
licheniformis,
Microbacterium
lacticum,
Calidifontibacter
indicus
were
isolated
from
the
surface
dairy
pasteuriser
after
cleaning
disinfection.
These
bacteria
produced
3.13-fold
more
mass
compared
to
sum
masses
monoculture.
The
present
study
confirms
observed
synergy
results
dynamic
social
interactions,
encompassing
commensalism,
exploitation,
amensalism.
M.
lacticum
appears
be
keystone
species
as
it
increased
growth
all
other
led
mass.
Interactions
among
three
(in
absence
lacticum)
also
contributed
towards
Biofilm
inducing
effects
cell-free-supernatants
for
some
combinations,
revealing
nature
synergy,
addition
additional
dual-species
combinations
confirmed
presence
higher-order
within
community.
Our
findings
provide
understanding
which
can
used
an
interaction–mediated
approach
cultivating,
engineering,
designing
synthetic
communities.
