The Listeria monocytogenes persistence factor ClpL is a potent stand-alone disaggregase DOI Open Access

Valentin Bohl,

Nele Merret Hollmann,

Tobias Melzer

et al.

Published: Nov. 17, 2023

Heat stress can cause cell death by triggering the aggregation of essential proteins. In bacteria, aggregated proteins are rescued canonical Hsp70/AAA+ (ClpB) bi-chaperone disaggregase. Man-made, severe conditions applied during e.g. food-processing represent a novel threat for bacteria exceeding capacity Hsp70/ClpB system. Here, we report on potent autonomous AAA+ disaggregase ClpL from Listeria monocytogenes that provides enhanced heat resistance to food-borne pathogen enabling persistence in adverse environments. shows increased thermal stability and disaggregation power compared Hsp70/ClpB, it withstand solubilize tight aggregates. binds protein aggregates via aromatic residues present its N-terminal domain (NTD) adopts partially folded dynamic conformation. Target specificity is achieved simultaneous interactions multiple NTDs with aggregate surface. remarkable structural plasticity forming diverse higher assembly states through interacting rings. become largely sequestered upon ring interactions. Stabilizing assemblies engineered disulfide bonds strongly reduces activity, suggesting they storage states.

Language: Английский

Virulence Potential and Antimicrobial Resistance of Listeria monocytogenes Isolates Obtained from Beef and Beef-Based Products Deciphered Using Whole-Genome Sequencing DOI Creative Commons
Ayanda Manqele, Abiodun A. Adesiyun, Thendo Mafuna

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(6), P. 1166 - 1166

Published: June 8, 2024

Listeria monocytogenes is a ubiquitous bacterial pathogen that threatens the food chain and human health. In this study, whole-genome sequencing (WGS) was used for genomic characterization of L. (n = 24) from beef beef-based products. Multilocus Sequence Type (MLST) analysis revealed ST204 CC204 most common sequence type (ST). Other types detected included ST1 ST876 CC1, ST5 CC5, ST9 CC9, ST88 CC88, ST2 ST1430 CC2, ST321 CC321. Genes encoding virulence factors complete LIPI-1 (pfrA-hly-plcA-plcB-mpl-actA) 54% (13/24) isolates ST204, ST321, ST1430, internalin genes inlABC were present in all STs. All STs carried four intrinsic/natural resistance genes, fosX, lin, norB, mprF, conferring to fosfomycin, lincosamide, quinolones, cationic peptides, respectively. Plasmids pLGUG1 J1776 (54% each), followed by pLI100 (13%) pLM5578 (7%). The prophage profile, vB_LmoS_188, overrepresented amongst isolates, LP_101, LmoS_293_028989, LP_030_2_021539, A006, LP_HM00113468. island 2 (LGI-2) found be while 3 (LGI-3) subset (25%). VII secretion system 42% sortase A genomes. Mobile genetic elements islands did not harbor any virulence, resistance, or environmental adaptation may benefit monocytogenes. carry confer first-line antibiotics treatment listeriosis. our study highlighted potential risk posed public, as bacterium frequently processing environments.

Language: Английский

Citations

4
Phage-Based Control of Listeria innocua in the Food Industry: A Strategy for Preventing Listeria monocytogenes Persistence in Biofilms DOI Creative Commons

Anna Zawiasa,

Marcin Schmidt, Agnieszka Olejnik‐Schmidt

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 482 - 482

Published: March 27, 2025

Listeria innocua, though considered non-pathogenic, frequently coexists with monocytogenes in industrial environments, aiding its survival biofilms. These biofilms pose a significant challenge food processing facilities, as they protect bacteria from disinfectants and facilitate their spread. The aim of this review was to identify bacteriophages promising method for eliminating the industry. Lytic show great potential combating Commercially available products, such PhageGuard Listex™ (P100) (Micreos Food Safety, Wageningen, Netherlands), effectively reduce both L. innocua products on production surfaces. Additionally, phage-derived enzymes, endolysins, can degrade biofilms, without compromising quality. following article highlights that although present biocontrol method, further research is necessary assess long-term effectiveness, particularly regarding bacterial resistance. To maximize efficacy, combination strategies phage cocktails recommended enhance biofilm eradication minimize contamination risks.

Language: Английский

Citations

0
Genomic Characterization of Listeria innocua Isolates Recovered from Cattle Farms, Beef Abattoirs, and Retail Outlets in Gauteng Province, South Africa DOI Creative Commons
James Gana, Nomakorinte Gcebe, Rian Pierneef

et al.

Pathogens, Journal Year: 2023, Volume and Issue: 12(8), P. 1062 - 1062

Published: Aug. 18, 2023

Whole-genome sequencing (WGS) was used for the genomic characterization of one hundred and ten strains Listeria innocua (L. innocua) isolated from twenty-three cattle farms, eight beef abattoirs, forty-eight retail outlets in Gauteng province, South Africa. In silico multilocus sequence typing (MLST) to identify isolates’ types (STs). BLAST-based analyses were antimicrobial virulence genes. The study also linked detection genes origin (industries samples) L. isolates. detected 14 STs, 13 resistance genes, 23 Of STs detected, ST637 (26.4%), ST448 (20%), 537 (13.6%), 1085 (12.7%) predominant, frequency varied significantly (p < 0.05). All 110 isolates carriers or more with lin (100%), fosX tet(M) (30%) being most frequently recognized, (clpC, clpE, clpP, hbp1, svpA, hbp2, iap/cwhA, lap, lpeA, lplA1, lspA, oatA, pdgA, prsA2) found all innocua. Overall, diversity significant differences frequencies resistance, according origins (source sample type) This, first recovered three levels/industries (farm, abattoir, retail) production system Africa, provides data on organism’s distribution potential food safety implications.

Language: Английский

Citations

8
Pathogenicity of Streptococcus iniae causing mass mortalities of yellow catfish (Tachysurus fulvidraco) and its induced host immune response DOI Creative Commons

Hongsen Xu,

Nengbin Zhu,

Yi‐Ling Chen

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: March 22, 2024

The outbreak of mass mortality occurred in Tachysurus fulvidraco farm Hubei province China. pathogenic strain Streptococcus iniae (termed 2022SI08) was isolated and identified from diseased T. , based on morphological, physiological, biochemical characteristics, as well 16S rRNA gene sequence phylogenetic analysis. Further, the whole genome isolate S. sequenced predicted to contain one single circular chromosome 1,776,777 bp with a GC content 37.14%. genomic analysis showed that 2022SI08 positive for 204 virulent 127 antibiotic resistant genes. experimental challenge demonstrated high pathogenicity retrieved median lethal dosage (LD 50 ) 9.53 × 10 5 CFU/g. Histopathological examination indicated could induce extensive tissue cell degeneration, necrosis, hemorrhage, inflammation skin, gill, fin, spleen, liver, kidney, intestine, eye, brain. Moreover, innate immune enzyme activities serum such acid phosphatase alkaline were increased significantly at 24 48 h post infection (hpi) then decreased 168 hpi. transcriptional profile associated following bacterial detected each point time, results revealed clear activation those genes, which proving their reacting regulatory role during response host against infection. an etiological agent mortalities this research will be conducive increasing our understanding pathogenesis defensive system invasion.

Language: Английский

Citations

3
When the going gets tough, the tough get going—Novel bacterial AAA+ disaggregases provide extreme heat resistance DOI Creative Commons

Valentin Bohl,

Axel Mogk

Environmental Microbiology, Journal Year: 2024, Volume and Issue: 26(7)

Published: July 1, 2024

Abstract Heat stress can lead to protein misfolding and aggregation, potentially causing cell death due the loss of essential proteins. Bacteria, being particularly exposed environmental stress, are equipped with disaggregases that rescue these aggregated The bacterial Hsp70 chaperone DnaK A TPase a ssociated diverse cellular ctivities ClpB form canonical disaggregase in bacteria. While this combination operates effectively during physiological heat it is ineffective against massive aggregation caused by temperature‐based sterilization protocols used food industry clinics. This leaves bacteria unprotected thermal processes. However, withstand extreme, man‐made conditions have emerged. These possess novel disaggregases, ClpG ClpL, which key players extreme resistance. present selected Gram‐negative or Gram‐positive bacteria, respectively, function superiorly exhibiting increased stability enhanced threading power compared DnaK/ClpB. enables ClpL operate at temperatures process large tight aggregates, thereby contributing genes for often encoded on mobile genomic islands conjugative plasmids, allowing their rapid spread among via horizontal gene transfer. threatens efficiency protocols. In review, we describe various identified date, characterizing commonalities specific features enable provide protection conditions.

Language: Английский

Citations

2
Soybean Glycinin’s Antibacterial Properties Provide a Feasible Natural Alternative for Improving the Overall Quality and Shelf-Life of Beef Steaks and Combating Foodborne Pathogens DOI Creative Commons

Mai Elsheikh,

Ali Osman, shimaa Edris

et al.

Food and Bioprocess Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 22, 2024

Abstract Ensuring the safety and longevity of food products is a major priority in meat industry. This paper focused on assessing soybean-glycinin (SBG) impact quality shelf-life beef Musculus longissimus thoracis et lumborum ( LTL ) steaks their potential in-vivo adverse consequences foodborne pathogens quantitative minimum biocidal values. Over 15 chilling-days at 4 °C, fresh -steaks were fortified with either 1 mg/g SBG (SBG1) or 0.5 (SBG0.5) compared to control for physicochemical, oxidative, microbiological attributes. Antibiotic-susceptible pathogens, Staphylococcus aureus, Salmonella enterica , Escherichia coli suppressed by 0.98 mg/mL SBG, whereas antibiotic-resistant required higher dosages 50 6.25 mg/mL, respectively. SBG-fortification kept -steaks’ pH below borderline until day 12, increased water-holding capacity, reduced cooking loss storage end, significantly improved tenderness p < 0.05). lightness, redness, chroma statistically unaffected SBG1.0-fortification control; however, there notable growing-patterns during last two storage-periods Except sixth- ninth-day storage-periods, yellowness hue control, indicating brighter, less red had dose-dependent antioxidant bactericidal both native microflora inoculated Listeria monocytogenes . Conclusively, activities improve oxidative stability beyond days inhibit inoculated-pathogens growth one log CFU/g above inoculation dose, validating as promising feasible preservation strategy.

Language: Английский

Citations

2
The Listeria monocytogenes persistence factor ClpL is a potent stand-alone disaggregase DOI Creative Commons

Valentin Bohl,

Nele Merret Hollmann,

Tobias Melzer

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Nov. 17, 2023

Heat stress can cause cell death by triggering the aggregation of essential proteins. In bacteria, aggregated proteins are rescued canonical Hsp70/AAA+ (ClpB) bi-chaperone disaggregase. Man-made, severe conditions applied during, e.g., food processing represent a novel threat for bacteria exceeding capacity Hsp70/ClpB system. Here, we report on potent autonomous AAA+ disaggregase ClpL from Listeria monocytogenes that provides enhanced heat resistance to food-borne pathogen enabling persistence in adverse environments. shows increased thermal stability and disaggregation power compared Hsp70/ClpB, it withstand solubilize tight aggregates. binds protein aggregates via aromatic residues present its N-terminal domain (NTD) adopts partially folded dynamic conformation. Target specificity is achieved simultaneous interactions multiple NTDs with aggregate surface. remarkable structural plasticity forming diverse higher assembly states through interacting rings. become largely sequestered upon ring interactions. Stabilizing assemblies engineered disulfide bonds strongly reduces activity, suggesting they storage states.

Language: Английский

Citations

4
Comparative genomic analysis of pathogenic factors of Listeria spp. using whole-genome sequencing DOI Creative Commons

Yumei Qi,

Qing Cao,

Xuehui Zhao

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 7, 2024

Listeria monocytogenes is an important foodborne pathogen known for causing listeriosis. To gain insights into the pathogenicity, genetic characterization, and evolution of various species, in vitro cell adhesion invasion ability assays whole-genome sequencing were performed using four strains isolated from livestock poultry slaughterhouses. The exhibited abilities Caco-2 RAW264.7 cells. Pathogenic Liv1-1 Lm2-20 had higher ability, but non-pathogenic Lin4-99 was more invasive than (p < 0.05). Genetic characterization revealed presence a single chromosome without plasmid across with similar sizes G + C% content. Analysis key pathogenic genes underscored multiple virulence among strains. In contrast, lacked LIPI-1, LIPI-2, LIPI-3 genes, which could possibly be cause their non-pathogenicity despite abilities. Thus, determinants do not necessarily predict and/or vitro. This study presents comprehensive comparative genome-wide analysis strains, offering invaluable pathogenesis genus.

Language: Английский

Citations

1
The Listeria monocytogenes persistence factor ClpL is a potent stand-alone disaggregase DOI Open Access

Valentin Bohl,

Nele Merret Hollmann,

Tobias Melzer

et al.

Published: March 20, 2024

Heat stress can cause cell death by triggering the aggregation of essential proteins. In bacteria, aggregated proteins are rescued canonical Hsp70/AAA+ (ClpB) bi-chaperone disaggregase. Man-made, severe conditions applied during e.g. food-processing represent a novel threat for bacteria exceeding capacity Hsp70/ClpB system. Here, we report on potent autonomous AAA+ disaggregase ClpL from Listeria monocytogenes that provides enhanced heat resistance to food-borne pathogen enabling persistence in adverse environments. shows increased thermal stability and disaggregation power compared Hsp70/ClpB, it withstand solubilize tight aggregates. binds protein aggregates via aromatic residues present its N-terminal domain (NTD) adopts partially folded dynamic conformation. Target specificity is achieved simultaneous interactions multiple NTDs with aggregate surface. remarkable structural plasticity forming diverse higher assembly states through interacting rings. become largely sequestered upon ring interactions. Stabilizing assemblies engineered disulfide bonds strongly reduces activity, suggesting they storage states.

Language: Английский

Citations

0
The Listeria monocytogenes persistence factor ClpL is a potent stand-alone disaggregase DOI Creative Commons

Valentin Bohl,

Nele Merret Hollmann,

Tobias Melzer

et al.

eLife, Journal Year: 2024, Volume and Issue: 12

Published: April 10, 2024

Heat stress can cause cell death by triggering the aggregation of essential proteins. In bacteria, aggregated proteins are rescued canonical Hsp70/AAA+ (ClpB) bi-chaperone disaggregase. Man-made, severe conditions applied during, e.g., food processing represent a novel threat for bacteria exceeding capacity Hsp70/ClpB system. Here, we report on potent autonomous AAA+ disaggregase ClpL from Listeria monocytogenes that provides enhanced heat resistance to food-borne pathogen enabling persistence in adverse environments. shows increased thermal stability and disaggregation power compared Hsp70/ClpB, it withstand solubilize tight aggregates. binds protein aggregates via aromatic residues present its N-terminal domain (NTD) adopts partially folded dynamic conformation. Target specificity is achieved simultaneous interactions multiple NTDs with aggregate surface. remarkable structural plasticity forming diverse higher assembly states through interacting rings. become largely sequestered upon ring interactions. Stabilizing assemblies engineered disulfide bonds strongly reduces activity, suggesting they storage states.

Language: Английский

Citations

0