Genomic, Functional, and Metabolic Enhancements in Multidrug-ResistantEnterobacter bugandensisFacilitating its Persistence and Succession in the International Space Station DOI Open Access
Pratyay Sengupta,

Shobhan Karthick Muthamilselvi Sivabalan,

Nitin K. Singh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 7, 2023

Abstract Background The International Space Station (ISS) stands as a testament to human achievement in space exploration. Despite its highly controlled environment, characterised by microgravity, increased CO 2 levels, and elevated solar radiation, microorganisms occupy unique niche. These microbial inhabitants play significant role influencing the health well-being of astronauts on board. One microorganism particular interest our study is Enterobacter bugandensis , primarily found clinical specimens including gastrointestinal tract, also reported possess pathogenic traits, leading plethora infections. Results Distinct from their Earth counterparts, ISS E. strains have exhibited resistance mechanisms that categorize them within ESKAPE pathogen group, collection pathogens recognized for formidable antimicrobial treatments. During two-year Microbial Tracking 1 mission, 12 multidrug resistant were isolated various locations ISS. We carried out comprehensive understand genomic intricacies ISS-derived comparison terrestrial strains, with keen focus those associated unravel evolutionary trajectories pivotal genes, especially contributing functional adaptations potential resistance. A hypothesis central was singular nature stresses distinct any Earth, could be driving these adaptations. Extending investigation, we meticulously mapped prevalence distribution across over time. This temporal analysis provided insights into persistence, succession, patterns colonization space. Furthermore, leveraging advanced analytical techniques, metabolic modelling, delved coexisting communities alongside multiple missions spatial locations. exploration revealed intricate interactions, offering window ecosystem dynamics Conclusions Our illuminated not only ways interactions sculpt diversity but factors might contribute dominance succession environment. implications findings are two-fold. Firstly, they shed light behavior, adaptation, evolution extreme, environments. Secondly, underscore need robust preventive measures, ensuring safety mitigating risks threats.

Language: Английский

Validation List no. 214. Valid publication of new names and new combinations effectively published outside the IJSEM DOI Open Access
Aharon Oren, Markus Göker

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2023, Volume and Issue: 73(11)

Published: Dec. 1, 2023

Microbiology Society journals contain high-quality research papers and topical review articles. We are a not-for-profit publisher we support invest in the microbiology community, to benefit of everyone. This supports our principal goal develop, expand strengthen networks available members so that they can generate new knowledge about microbes ensure it is shared with other communities.

Language: Английский

Citations

18
Genomic, functional, and metabolic enhancements in multidrug-resistant Enterobacter bugandensis facilitating its persistence and succession in the International Space Station DOI Creative Commons
Pratyay Sengupta,

Shobhan Karthick Muthamilselvi Sivabalan,

Nitin K. Singh

et al.

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: March 23, 2024

Abstract Background The International Space Station (ISS) stands as a testament to human achievement in space exploration. Despite its highly controlled environment, characterised by microgravity, increased CO $$_{2}$$ 2 levels, and elevated solar radiation, microorganisms occupy unique niche. These microbial inhabitants play significant role influencing the health well-being of astronauts on board. One microorganism particular interest our study is Enterobacter bugandensis , primarily found clinical specimens including gastrointestinal tract, also reported possess pathogenic traits, leading plethora infections. Results Distinct from their Earth counterparts, ISS E. strains have exhibited resistance mechanisms that categorise them within ESKAPE pathogen group, collection pathogens recognised for formidable antimicrobial treatments. During 2-year Microbial Tracking 1 mission, 13 multidrug-resistant were isolated various locations ISS. We carried out comprehensive understand genomic intricacies ISS-derived comparison terrestrial strains, with keen focus those associated unravel evolutionary trajectories pivotal genes, especially contributing functional adaptations potential resistance. A hypothesis central was singular nature stresses distinct any Earth, could be driving these adaptations. Extending investigation, we meticulously mapped prevalence distribution across over time. This temporal analysis provided insights into persistence, succession, patterns colonisation space. Furthermore, leveraging advanced analytical techniques, metabolic modelling, delved coexisting communities alongside multiple missions spatial locations. exploration revealed intricate interactions, offering window ecosystem dynamics Conclusions Our illuminated not only ways interactions sculpt diversity but factors might contribute dominance succession environment. implications findings are twofold. Firstly, they shed light behaviour, adaptation, evolution extreme, environments. Secondly, underscore need robust preventive measures, ensuring safety mitigating risks threats.

Language: Английский

Citations

8
Cloning and Heterologous Expression of a Novel Thermo-Alkalistable GH-10 Xylanase (rXyn-GM) Retrieved from Tapovan Hot-Spring Soil Metagenome and its Characterization for Kinetic Parameters DOI
Garima Chauhan,

Nivedita Bisht,

Poonam Gautam

et al.

Indian Journal of Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Language: Английский

Citations

0
Genomic description of Microbacterium mcarthurae sp. nov., a bacterium collected from the International Space Station that exhibits unique antimicrobial-resistant and virulent phenotype DOI Creative Commons
Megan Hill,

Vanessa R. Minnis,

Anna Simpson

et al.

mSystems, Journal Year: 2025, Volume and Issue: unknown

Published: May 20, 2025

ABSTRACT A novel bacterial strain, designated as 1F8SW-P5 T , was isolated from the wall of crew quarters on International Space Station. Cells were Gram-staining-positive, strictly aerobic, non-spore-forming, chemoheterotrophic, and mesophilic rods exhibiting catalase-positive oxidase-negative reactivity. Strain shared highest 16S rRNA gene similarity with Microbacterium proteolyticum CECT 8356 (99.34%) gyrB algihabitans KSW2-21 (91.34%). Its strongest matches via average nucleotide identity DNA–DNA hybridization to hydrothermale CGMCC_1.12512 (84.36% 25.80%, respectively). formed a distinct lineage during phylogenetic phylogenomic analysis. The biochemical, phenotypic, chemotaxonomic, features substantiated affiliation new species for which we propose name mcarthurae type strain (=DSM 115934 =NRRL B-65667 ). Based metagenomic data collected Microbial Tracking mission series, M. identified all surfaces ( n = 8) over an 8-year period, increase in relative abundance time. This is potential concern, observed resistance tested fluoroquinolone antibiotics 6), two β-lactam antibiotics, one macrolide antibiotic, not predicted based isolate or plasmid genotype alone. Furthermore, found virulence, compared Escherichia coli when within Caenorhabditis elegans model. pathogenic profile highlights importance continued characterization spacecraft-associated microbes, previously unidentified antimicrobial virulence genes, implementation targeted mitigation strategies spaceflight. IMPORTANCE Crew members are at increased risk exposure infection by microbes Therefore, it imperative characterize that able colonize persist spacecraft, how those organisms change distribution time, their genotypic phenotypic expression traits (i.e., whether they encode exhibit associated antibiotic and/or virulence). Here, describe Station (ISS), . be distributed throughout ISS Additionally, this bacterium exhibits unique phenotype whole-genome sequencing, well suggesting need identification undescribed genes monitoring/mitigation

Language: Английский

Citations

0
Identification of a novel xanthan-binding module of a multi-modular Cohnella sp. xanthanase DOI Creative Commons

Rui Han,

Melanie Baudrexl, Christina Ludwig

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: March 26, 2024

A new strain of xanthan-degrading bacteria identified as Cohnella sp. has been isolated from a xanthan thickener for food production. The was able to utilize the only carbon source and reduce viscosity xanthan-containing medium during cultivation. Comparative analysis secretomes after growth on different media led identification xanthanase designated Csp Xan9, which recombinant production in Escherichia coli . Xan9 could efficiently degrade β-1,4-glucan backbone previous removal pyruvylated mannose residues ends native side chains by lyase treatment (XLT-xanthan). Compared with Paenibacillus nanensis , had module composition at N- C-terminal ends. main putative oligosaccharides released XLT-xanthan cleavage were tetrasaccharides octasaccharides. To explore functions regions enzyme, truncated variants lacking some non-catalytic modules ( Xan9-C, Xan9-N, Xan9-C-N) produced. Enzyme assays purified deletion derivatives, all contained catalytic glycoside hydrolase family 9 (GH9) module, demonstrated substantially reduced specific activity Xan9-C-N compared full-length Xan9. found represent novel carbohydrate-binding CBM66 binding affinity XLT-xanthan, shown polyacrylamide gel electrophoresis presence various polysaccharides. previously known function member is exo-type non-reducing fructose β-fructan polysaccharides inulin levan.

Language: Английский

Citations

1
Rhinocladiella similis: A Model Eukaryotic Organism for Astrobiological Studies on Microbial Interactions with Martian Soil Analogs DOI Creative Commons
Alef dos Santos, Júnia Schultz, Isabella Dal’Rio

et al.

JACS Au, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

The exploration of our solar system for microbial extraterrestrial life is the primary goal several space agencies. Mars has attracted substantial attention owing to its Earth-like geological history and potential niches life. This study evaluated suitability polyextremophilic fungal strain Rhinocladiella similis LaBioMMi 1217 as a model eukaryote astrobiology. Comprehensive genomic analysis, including taxonomic functional characterization, revealed R. genes conferring resistance Martian-like stressors, such osmotic pressure ultraviolet radiation. When cultured in synthetic Martian regolith (MGS-1), exhibited altered morphology produced unique metabolites, oxylipins, indolic acid derivatives, siderophores, which might be biosignatures. Notably, oxylipins were detected using laser desorption ionization mass spectrometry, technique slated use upcoming European Space Agency ExoMars mission. Our findings enhance understanding extremophilic metabolism under conditions, supporting black yeasts viable eukaryotic models astrobiological studies. Further research necessary validate these biosignatures explore broader applicability other environments.

Language: Английский

Citations

0
Description of Cohnella rhizoplanae sp. nov., isolated from the root surface of soybean (Glycine max) DOI Creative Commons
Peter Kämpfer, Stefanie P. Glaeser, John A. McInroy

et al.

Antonie van Leeuwenhoek, Journal Year: 2024, Volume and Issue: 118(2)

Published: Feb. 1, 2024

Language: Английский

Citations

0
Genomic, Functional, and Metabolic Enhancements in Multidrug-ResistantEnterobacter bugandensisFacilitating its Persistence and Succession in the International Space Station DOI Open Access
Pratyay Sengupta,

Shobhan Karthick Muthamilselvi Sivabalan,

Nitin K. Singh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 7, 2023

Abstract Background The International Space Station (ISS) stands as a testament to human achievement in space exploration. Despite its highly controlled environment, characterised by microgravity, increased CO 2 levels, and elevated solar radiation, microorganisms occupy unique niche. These microbial inhabitants play significant role influencing the health well-being of astronauts on board. One microorganism particular interest our study is Enterobacter bugandensis , primarily found clinical specimens including gastrointestinal tract, also reported possess pathogenic traits, leading plethora infections. Results Distinct from their Earth counterparts, ISS E. strains have exhibited resistance mechanisms that categorize them within ESKAPE pathogen group, collection pathogens recognized for formidable antimicrobial treatments. During two-year Microbial Tracking 1 mission, 12 multidrug resistant were isolated various locations ISS. We carried out comprehensive understand genomic intricacies ISS-derived comparison terrestrial strains, with keen focus those associated unravel evolutionary trajectories pivotal genes, especially contributing functional adaptations potential resistance. A hypothesis central was singular nature stresses distinct any Earth, could be driving these adaptations. Extending investigation, we meticulously mapped prevalence distribution across over time. This temporal analysis provided insights into persistence, succession, patterns colonization space. Furthermore, leveraging advanced analytical techniques, metabolic modelling, delved coexisting communities alongside multiple missions spatial locations. exploration revealed intricate interactions, offering window ecosystem dynamics Conclusions Our illuminated not only ways interactions sculpt diversity but factors might contribute dominance succession environment. implications findings are two-fold. Firstly, they shed light behavior, adaptation, evolution extreme, environments. Secondly, underscore need robust preventive measures, ensuring safety mitigating risks threats.

Language: Английский

Citations

0