The influence and relationship of dysbiosis in the urinary microbiota on patients with urolithiasis DOI Creative Commons
Hsiang‐Ying Lee, Chung‐Chi Lin,

Yung‐Shun Juan

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Abstract Background Urolithiasis is a disease with high prevalence and recurrence rate. There are various risk factors impacting on stone formation including intestinal micorbiome. This study aims to investigate the relationship between urine microbiota urolithiasis. Methods We collected mid-stream voided samples from urolithiasis patients control participants stored them in freezer at -80°C. All enrolled were requested provide information about their clinical characteristics. Each step of procedure, extraction genomic DNA samples; amplification by polymerase chain reaction (PCR); PCR product quantification, mixing, purification; library preparation; sequencing was performed quality (QC) measures. Alpha diversity indicative species complexity within individual samples, beta analysis used evaluate differences among terms complexity. Results 28 59 who reported no recent antibiotic usage. In analysis, there significant difference groups according ANOSIM statistical analysis. (P = 0.004). On comparing groups, it showed Alcaligenes, Bacteroides, Blautia, Ruminococcaceae_UCG, Cutibacterium, Alistipes, Lachnoclostridium present more group than patients. Conclusions Our current shows that dysbiosis may be related development Further research targeting specific microbes identify role diseases necessary might novel diagnostic biomarkers therapeutic options.

Language: Английский

The causal relationship between gut microbiota and biliary tract cancer: comprehensive bidirectional Mendelian randomization analysis DOI Creative Commons
Kui Wang,

Suijian Wang,

Xianzheng Qin

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: March 15, 2024

Background Growing evidence has shown that gut microbiome composition is associated with Biliary tract cancer (BTC), but the causality remains unknown. This study aimed to explore causal relationship between microbiota and BTC, conduct an appraisal of microbiome’s utility in facilitating early diagnosis BTC. Methods We acquired summary data for Genome-wide Association Studies (GWAS) pertaining BTC (418 cases 159,201 controls) from Biobank Japan (BBJ) database. Additionally, GWAS relevant (N = 18,340) were sourced MiBioGen consortium. The primary methodology employed analysis consisted Inverse Variance Weighting (IVW). Evaluations sensitivity carried out through utilization multiple statistical techniques, encompassing Cochrane’s Q test, MR-Egger intercept evaluation, global test MR-PRESSO, a leave-one-out methodological analysis. Ultimately, reverse Mendelian Randomization was conducted assess potential reciprocal causality. Results outcomes derived IVW substantiated presence Family Streptococcaceae (OR 0.44, P 0.034), Veillonellaceae 0.46, 0.018), Genus Dorea 0.29, 0.041) exerted protective influence against Conversely, Class Lentisphaeria 2.21, 0.017), Lachnospiraceae FCS020 Group 2.30, 0.013), Order Victivallales 0.017) adverse impact. To any effect, we used as exposure outcome, this revealed associations five different types microbiota. disclosed absence empirical indicators either heterogeneity or pleiotropy. Conclusion investigation represents inaugural identification indicative supporting beneficial detrimental relationships risk determined MR methodologies. These could hold significance formulation individualized therapeutic strategies at prevention survival enhancement.

Language: Английский

Citations

9
Unraveling the association of bacteria and urinary stones in patients with urolithiasis: an update review article DOI Creative Commons
Abdollah Razi, Azita Ghiaei,

Fahimeh Kamali Dolatabadi

et al.

Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11

Published: Aug. 30, 2024

Urinary stone disease (USD) is a prevalent urological condition, ranking as one of the most common urinary tract disorders globally. Various risk factors influence formation kidney stones, and recent research indicates rising prevalence urolithiasis worldwide, particularly in developing countries. While morbidity associated with stones has decreased years, long-term complications such recurrence, failure, uremia continue to burden patients. Understanding etiologies urolithiasis, including role bacteria, crucial they can contribute recurrence. The incidence infection (UTI) be attributed specific infectious factors, socio-demographic comorbid metabolic disorders. This review article explores emerging evidence suggesting involvement bacteria USD. It discusses potential microorganisms non-infection highlights association between UTIs urolithiasis. Furthermore, it surveys relationship recurrent bacterial biofilms UTIs. Considering various biochemical analysis presence essential for treating patients optimally. aims provide an updated understanding shedding light on pathophysiology formation, characteristics, microbiome stones.

Language: Английский

Citations

5
Impact of gut microbiota on nonalcoholic fatty liver disease: insights from a leave-one-out cross-validation study DOI Creative Commons
Tongtong Pan, Lihuang Su, Yiying Zhang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 8, 2024

Introduction Enteric dysbacteriosis is strongly associated with nonalcoholic fatty liver disease (NAFLD). However, the underlying causal relationship remains unknown. Thus, present study aimed to investigate between gut microbiota and NAFLD using Mendelian randomization (MR) analyze target genes potentially regulated by specific microbiota. Methods Bidirectional two-sample MR analysis was performed inverse variance weighted (IVW) supplemented MR-Egger, median, simple mode, mode methods. Data were pooled from association studies. The least absolute shrinkage, selection operator regression, Support Vector Machine algorithm used identify these intestinal flora in NAFLD. expression of verified methionine choline-deficient (MCD) diet-fed mice. Results IVW results confirmed a eight microbes Notably, order Actinomycetales, NB1n, family Actinomycetaceae, Oxalobacteraceae genus Ruminococcaceae UCG005 positively correlated, whereas Lactobacillaceae, Christensenellaceae R7 group , Intestinibacter negatively correlated onset. In NAFLD, bacteria four genes: colony-stimulating factor 2 receptor β, fucosyltransferase 2, 17-beta-hydroxysteroid dehydrogenase 14, microtubule affinity regulatory kinase 3 ( MAPK3 ). All genes, except MARK3 differentially expressed tissues MCD Discussion abundance species progression displayed based on genes. Our findings contributed advancement microecology-based diagnostic technologies targeted therapies for

Language: Английский

Citations

4
Gut microbiota and autoimmune neurologic disorders: a two-sample bidirectional Mendelian randomization study DOI Creative Commons
Mengyuan Zhang, Jie Fang,

Chamou Zheng

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 24, 2024

Increasing evidence has suggested that alterations in the gut microbiome are correlated with autoimmune neurologic disorders, yet causal relationship between them to be established.

Language: Английский

Citations

4
The influence and relationship of dysbiosis in the urinary microbiota on patients with urolithiasis DOI
Hsiang‐Ying Lee, Chung‐Chi Lin,

Yung‐Shun Juan

et al.

Urolithiasis, Journal Year: 2025, Volume and Issue: 53(1)

Published: March 19, 2025

Language: Английский

Citations

0
Genetic evidence on the causality between gut microbiota and various asthma phenotypes: a two-sample Mendelian randomization study DOI Creative Commons
Zi-Xuan Cheng,

Yixing Wu,

Zhijun Jie

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 13

Published: Jan. 11, 2024

Introduction Asthma is a multifarious disease that manifests in various phenotypes. Among the factors contribute to development of asthma, gut microbiota has recently emerged as compelling area investigation. This study aims investigate causal relationships between and distinct asthma Methods The genome-wide association (GWAS) summary statistics for 211 microbial taxa were used exposure. Five traits pertaining phenotypes (asthma, allergic childhood suggestive eosinophilic obesity-related asthma) included outcome. We conducted Mendelian randomization (MR) analysis sensitivity each bacterial Result discovered total 58 associations exhibited evidence causality. Out these, 4 remained significant even after applying multiple correction. An increased risk was causally associated with higher abundance genus Holdemanella (OR = 1.11; CI: 1.05-1.17; p 0.027), Oxalobacter 1.09; 1.04-1.15; 0.025) Butyricimonas 1.14; 1.06-1.22; 0.027). Order NB1n linked an 1.17; 1.07-1.29; 0.015). There limited overlap among potential Conclusion Our research provided genetic establishes phenotypes, supporting role It possible different play identified this require further

Language: Английский

Citations

3
Genetically predicted causality between gut microbiota, blood metabolites, and intracerebral hemorrhage: a bidirectional Mendelian randomization study DOI Creative Commons
Tianlong Zhang, Gang Liu,

Yina Cao

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 15, 2024

Background Recent research linked changes in the gut microbiota and serum metabolite concentrations to intracerebral hemorrhage (ICH). However, potential causal relationship remained unclear. Therefore, current study aims estimate effects of genetically predicted causality between microbiota, metabolites, ICH. Methods Summary data from genome-wide association studies (GWAS) ICH were obtained separately. Gut GWAS ( N = 18,340) acquired MiBioGen study, metabolites 7,824) TwinsUK KORA studies, summary-level for FinnGen R9 (ICH, 3,749 cases; 339,914 controls). A two-sample Mendelian randomization (MR) was conducted explore The random-effects inverse variance-weighted (IVW) MR analyses performed as primary results, together with a series sensitivity assess robustness results. Besides, reverse evaluate possibility causation. To validate relevant findings, we further selected UK Biobank analysis. Results analysis results revealed nominal p < 0.05) 17 microbial taxa, 31 Among higher level genus Eubacterium xylanophilum (odds ratio (OR): 1.327, 95% confidence interval (CI):1.154–1.526; Bonferroni-corrected 7.28 × 10 −5 ) retained strong risk after Bonferroni corrected test. Concurrently, Senegalimassilia (OR: 0.843, CI: 0.778–0.915; 4.10 associated lower risk. Moreover, correction, only two out initial metabolites. One Isovalerate 7.130, 2.648–19.199; 1.01 −4 showed very ICH, whereas other unidentified excluded Various yielded similar no heterogeneity or directional pleiotropy observed. Conclusion This significant influence on specific bacterial taxa engaged development identified. Further is required future delve deeper into mechanisms behind these findings.

Language: Английский

Citations

1
Can early gut microbiota screening reduce the incidence of cognitive impairment? A Mendelian randomization study DOI
Chongcheng Xi, Jie Zhang, Haihui Liu

et al.

Journal of Alzheimer s Disease, Journal Year: 2024, Volume and Issue: 102(1), P. 195 - 206

Published: Oct. 23, 2024

Gastrointestinal symptoms are now detected early in the clinical course of many dementia patients, and studies microbiome-gut-brain axis have confirmed bidirectional interactions between gut brain. However, causal relationship microbiota cognitive impairment has not been fully established. Therefore, this study conducted a Mendelian randomization to elucidate potential impairment.

Language: Английский

Citations

1
The influence and relationship of dysbiosis in the urinary microbiota on patients with urolithiasis DOI Creative Commons
Hsiang‐Ying Lee, Chung‐Chi Lin,

Yung‐Shun Juan

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Abstract Background Urolithiasis is a disease with high prevalence and recurrence rate. There are various risk factors impacting on stone formation including intestinal micorbiome. This study aims to investigate the relationship between urine microbiota urolithiasis. Methods We collected mid-stream voided samples from urolithiasis patients control participants stored them in freezer at -80°C. All enrolled were requested provide information about their clinical characteristics. Each step of procedure, extraction genomic DNA samples; amplification by polymerase chain reaction (PCR); PCR product quantification, mixing, purification; library preparation; sequencing was performed quality (QC) measures. Alpha diversity indicative species complexity within individual samples, beta analysis used evaluate differences among terms complexity. Results 28 59 who reported no recent antibiotic usage. In analysis, there significant difference groups according ANOSIM statistical analysis. (P = 0.004). On comparing groups, it showed Alcaligenes, Bacteroides, Blautia, Ruminococcaceae_UCG, Cutibacterium, Alistipes, Lachnoclostridium present more group than patients. Conclusions Our current shows that dysbiosis may be related development Further research targeting specific microbes identify role diseases necessary might novel diagnostic biomarkers therapeutic options.

Language: Английский

Citations

0