Characterisation of the bacteriomes harboured by major wireworm pest species in the Canadian Prairies DOI Creative Commons
Ivan Drahun,

Keagan Morrison,

Elise A. Poole

et al.

Insect Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 9, 2024

Abstract Nearly all insects harbour bacterial communities that can have a profound effect on their life history, including regulating and shaping host metabolism, development, immunity fitness. The bacteriomes of several coleopterans been described; however, very little has reported for wireworms. These long‐lived larvae click beetles (Coleoptera: Elateridae) are major agricultural pests variety crops grown in the Canadian Prairies. Consequently, goal this study was to characterise five most significant pest species within region: Limonius californicus , Hypnoidus abbreviatus H. bicolor Aeolus mellillus Dalopius spp. To do this, we collected from southern Manitoba fields (pre‐seeding) carried out 16S rRNA sequencing individual specimens. Our results indicate wireworms diverse taxon‐rich communities, with over 400 genera identified predominately phyla Proteobacteria, Actinobacteriota, Bacteroidota Firmicutes. However, each had nine or fewer comprising >80% bacteriome. Network analyses revealed some community structuring consistent among species, which may culminate shaping/regulating biology. Moreover, microbial signatures were influenced by both ontogeny (early vs. late stage larvae) reproductive strategy (sexual parthenogenetic), myriad other factors likely contributing diversity impossible resolve our study. Overall, metagenomics represents first Prairies findings could assist development sustainable management strategies these important pests.

Language: Английский

Evidence for the independent evolution of a rectal complex within the beetle superfamily Scarabaeoidea DOI Creative Commons
Robin Beaven, Barry Denholm,

Maria Fremlin

et al.

Arthropod Structure & Development, Journal Year: 2025, Volume and Issue: 84, P. 101406 - 101406

Published: Jan. 1, 2025

Language: Английский

Citations

0
Effects of municipal wastewater effluents on the invertebrate microbiomes of an aquatic-riparian food web DOI Creative Commons
Emilie Diesbourg, Karen A. Kidd, Brittany G. Perrotta

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 125948 - 125948

Published: Feb. 1, 2025

Language: Английский

Citations

0
Thermal Stress and Its Effects on the Gut Microbiome of Parthenium Beetles DOI

P.K. Singh,

Prema Haldhar,

Tamal Das

et al.

Archives of Insect Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 118(4)

Published: April 8, 2025

ABSTRACT The gut microbiota plays a vital role in nutrient and energy utilization, as well the host's ability to adapt its immune system environmental changes. As biological control agent for invasive Parthenium weed, beetle Zygogramma bicolorata ( Z. ) Pallister is often exposed fluctuating temperatures, which may induce stress natural habitat. This study utilized 16S amplicon sequencing explore impact of temperature on microbiome under cold (15°C), (27°C), hot (35°C) conditions. A total 11 bacterial phyla 149 genera were identified, with Firmicutes, Proteobacteria, Cyanobacteria being most abundant. Temperature treatments significantly influenced diversity microbiota, evidenced by alpha measures. Principal coordinate analysis further revealed substantial variations composition across different Additionally, PICRUSt2 suggested that linked metagenomic functions related amino acid carbohydrate transport, inorganic ion metabolism, cellular processes. Our findings suggest thermal alters beetles, offering new insights into how these beetles have ecologically adapted fluctuations, while also highlighting potential microbes maintaining health stress.

Language: Английский

Citations

0
Evidence for the independent evolution of a rectal complex within the beetle superfamily Scarabaeoidea DOI Creative Commons
Robin Beaven, Barry Denholm,

Maria Fremlin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 29, 2024

Abstract Rectal or cryptonephridial complexes have evolved repeatedly in arthropods, including beetles where they occur ∼190,000 species of Cucujiformia and Bostrichoidea, Lepidoptera ∼160,000 species. Sections the Malpighian/renal tubules coat outer surface rectum, acting as powerful recycling systems gut contents, recovering water specific solutes. There are hints that a rectal complex independently within another beetle group, Scarabaeoidea. Here we report our observations Scarabaeoidea, which support this view. We did not find related Staphylinoidea, Lucanidae, basal group observe Melolontha melolontha (Melolonthini), Pachnoda marginata Cetonia aurata (Cetoniinae), consistent with previous reports from these groups. Intriguingly found adult, but M. larvae, larvae adults Cetoniinae, indicating dramatic pupal remodelling organ systems. Insights into structure Scarabaeoidea compared well-studied Cucujiformia. Finally discuss possible functions future approaches to address question.

Language: Английский

Citations

1
Unearthing Lactococcus lactis and Scheffersomyeces symbionts from edible wood-boring beetle larvae as a bio-resource for industrial applications DOI Creative Commons
Shadrack Kibet, Cynthia M. Mudalungu, Njogu M. Kimani

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 30, 2024

Abstract Background Gut microbiota have several advantages in influencing the host nutrition, metabolism, immunity and growth. However, understanding of gut key edible wood-boring beetle larvae remain largely undefined. In present study, characteristics two species ( Titocerus jaspideus Passalus punctiger ) from indigenous forested areas were investigated. Results Over 50% Amplicon Sequence Variants (ASVs) constituted Firmicutes T. . The dominant phyla both Bacteroidota (4.20–19.79%) Proteobacteria (15.10–23.90%). Lactococcus lactis was most abundant core prokaryote guts fungi identified insects belong to phylum Obazoa (66%) Ascomycota (> 15%). Scheffersomyeces sp. eukaryote recorded. diversity insect did not vary significantly. Most prokaryotic genes expressed predominantly associated with biosynthesis metabolism. Conclusion Our findings demonstrated that are microbes wood boring desirable probiotic properties promising use food product fermentation for improved growth performance, barrier health, intestinal flora balance immune protection human animals. Further studies highlight latest medical-based applications L. as live-delivery vector administration therapeutics against communicable non-communicable diseases warranted.

Language: Английский

Citations

1
The vector–symbiont affair: a relationship as (im)perfect as it can be DOI
Carla Polycarpo, Ana Beatriz Walter‐Nuno,

Leonan Azevedo-Reis

et al.

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 63, P. 101203 - 101203

Published: May 3, 2024

Language: Английский

Citations

0
Characterisation of the bacteriomes harboured by major wireworm pest species in the Canadian Prairies DOI Creative Commons
Ivan Drahun,

Keagan Morrison,

Elise A. Poole

et al.

Insect Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 9, 2024

Abstract Nearly all insects harbour bacterial communities that can have a profound effect on their life history, including regulating and shaping host metabolism, development, immunity fitness. The bacteriomes of several coleopterans been described; however, very little has reported for wireworms. These long‐lived larvae click beetles (Coleoptera: Elateridae) are major agricultural pests variety crops grown in the Canadian Prairies. Consequently, goal this study was to characterise five most significant pest species within region: Limonius californicus , Hypnoidus abbreviatus H. bicolor Aeolus mellillus Dalopius spp. To do this, we collected from southern Manitoba fields (pre‐seeding) carried out 16S rRNA sequencing individual specimens. Our results indicate wireworms diverse taxon‐rich communities, with over 400 genera identified predominately phyla Proteobacteria, Actinobacteriota, Bacteroidota Firmicutes. However, each had nine or fewer comprising >80% bacteriome. Network analyses revealed some community structuring consistent among species, which may culminate shaping/regulating biology. Moreover, microbial signatures were influenced by both ontogeny (early vs. late stage larvae) reproductive strategy (sexual parthenogenetic), myriad other factors likely contributing diversity impossible resolve our study. Overall, metagenomics represents first Prairies findings could assist development sustainable management strategies these important pests.

Language: Английский

Citations

0