Carbohydrate Research, Journal Year: 2024, Volume and Issue: 546, P. 109307 - 109307
Published: Nov. 6, 2024
Language: Английский
Microbial Genomics, Journal Year: 2025, Volume and Issue: 11(2)
Published: Feb. 5, 2025
Carbapenem-resistant Acinetobacter baumannii is recognized by the World Health Organization (WHO) as one of top priority pathogens. Despite its public health importance, genomic data clinical isolates from Malaysia remain scarce. In this study, whole-genome sequencing was performed on 126 A . collected main tertiary hospital in state Terengganu, Malaysia, over a 10-year period (2011–2020). Antimicrobial susceptibilities determined for 20 antibiotics belonging to 8 classes showed that 77.0% ( n =97/126) were categorized multidrug resistant (MDR), with all MDR being carbapenem resistant. Multilocus sequence typing analysis Terengganu A. into 34 Pasteur and 44 Oxford types (STs), ST2 Global Clone 2 lineage identified dominant ST =76/126; 60.3%). The could be subdivided six STs majority ST195 =35) ST208 =17). Various antimicrobial resistance genes bla OXA-23 -encoded carbapenemase predominant acquired gene =90/126; 71.4%). Plasmid-encoded rep nearly =122/126; 96.8%) Rep_3 family =121). virulence factors identified, highlighting pathogenic nature bacterium. Only 14/126 (11.1%) positive carriage CRISPR-Cas arrays none prevalent harbouring them. This study provided snapshot obtained single healthcare centre predominance closely related lineage, indicating entrenchment clone hospital.
Language: Английский
Citations
1
Journal of Bacteriology, Journal Year: 2024, Volume and Issue: 206(5)
Published: April 25, 2024
ABSTRACT Acinetobacter baumannii is highly resistant to antimicrobial agents, and XDR strains have become widespread. A. has developed resistance colistin, which considered the last resort against Gram-negative bacteria, mainly caused by lipooligosaccharide (LOS) phosphoethanolamine (pEtN) and/or galactosamine (GalN) modifications induced mutations that activate two-component system (TCS) pmrAB . Although PmrAB of been recognized as a drug factor, its function TCS, including regulatory genes response factors, not fully elucidated. In this study, clarify we elucidated (regulon) via transcriptome analysis using -activated mutant strains. We discovered responds low pH, Fe 2+ , Zn Al 3+ selectively recognizes rather than novel region ExxxE, in addition ExxE motif sequence, involved environmental response. Furthermore, participates modification LOS on bacterial surface metal ions such contributing attenuation toxicity development colistin polymyxin B This study demonstrates only regulates play an important role but also responses stimuli stimulation induces modification. reveals importance adaptation antibacterial emergence mechanisms IMPORTANCE Antimicrobial (AMR) pressing global issue human health. notably high World Health Organization’s list bacteria for new agents are urgently needed. Colistin one last-resort drugs used extensively drug-resistant (XDR) bacteria. However, increasingly primarily modifying activating PmrAB. comprehensively elucidates detailed mechanism well biological functions. Understanding molecular biology these molecules, serve factors recognition crucial developing fundamental solutions AMR problem.
Language: Английский
Citations
6
Viruses, Journal Year: 2024, Volume and Issue: 16(5), P. 771 - 771
Published: May 13, 2024
The genus Acinetobacter comprises both environmental and clinically relevant species associated with hospital-acquired infections. Among them, baumannii is a critical priority bacterial pathogen, for which the research development of new strategies antimicrobial treatment are urgently needed. spp. produce variety structurally diverse capsular polysaccharides (CPSs), surround cells thick protective layer. These surface structures primary receptors capsule-specific bacteriophages, that is, phages carrying tailspikes CPS-depolymerizing/modifying activities. Phage tailspike proteins (TSPs) exhibit hydrolase, lyase, or esterase activities toward corresponding CPSs certain structure. In this study, data on all lytic infecting genomes deposited in NCBI GenBank database by January 2024 were summarized. 149 identified TSPs encoded 143 phages, specificity (K specificity) 46 has been experimentally determined predicted previously. 63 CPSs, produced various K types, was study using bioinformatic analysis. A comprehensive phylogenetic analysis confirmed prediction revealed possibility genetic exchange gene regions to CPS-recognizing/degrading parts different between morphologically taxonomically distant groups phages.
Language: Английский
Citations
5
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: June 19, 2024
Abstract Many bacterial pathogens, including the human exclusive pathogen Salmonella Typhi, express capsular polysaccharides as a crucial virulence factor. Here, through S . Typhi whole genome sequence analyses and functional studies, we found list of single point mutations that make hypervirulent. We discovered mutation in Vi biosynthesis enzymes control polymerization or acetylation is enough to result different capsule variants Typhi. All variant strains are pathogenic, but hyper particularly hypervirulent, demonstrated by high morbidity mortality rates observed infected mice. The hypo have primarily been identified Africa, whereas distributed worldwide. Collectively, these studies increase awareness about existence establish solid foundation for numerous future on variants, offer valuable insights into strategies combat capsulated bacteria.
Language: Английский
Citations
5
Tetrahedron, Journal Year: 2025, Volume and Issue: unknown, P. 134537 - 134537
Published: Feb. 1, 2025
Language: Английский
Citations
0
The Journal of Organic Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: March 25, 2025
Herein, we communicate a concise synthetic strategy for the first total synthesis of O-antigenic tetrasaccharide repeating unit Acinetobacter lwoffii EK30A polysaccharide. The structurally unique possesses three consecutive 1,2-cis-glycosidic linkages and two rare sugar units, i.e., d-FucpNAc d-Quip4NAc. All functionalized monosaccharides were efficiently synthesized from naturally abundant inexpensive d-galactose to make route affordable convenient. was using highly stereoselective, convergent (1 + 2 1) carefully optimizing high-yielding glycosylation strategy.
Language: Английский
Citations
0
Carbohydrate Research, Journal Year: 2025, Volume and Issue: unknown, P. 109478 - 109478
Published: April 1, 2025
Language: Английский
Citations
0
Viruses, Journal Year: 2025, Volume and Issue: 17(5), P. 623 - 623
Published: April 26, 2025
Acinetobacter baumannii and Klebsiella pneumoniae are significant nosocomial pathogens worldwide. In this study, the general characterization of A. K. isolates obtained from blood intensive care unit patients multidisciplinary scientific practical center emergency medicine January to September 2024 was performed. Prophage regions prophage-derived tailspike polysaccharide-depolymerizing or -modifying enzymes within these were identified characterized in detail using a refined workflow. The protocol, encompassing comprehensive survey all predicted bacterial proteins, revealed an average 6.0 prophage per genome, including putatively derived filamentous phages, 4.8 isolate. Analysis putative indicated that most related previously isolated, yet unclassified, temperate phages infecting pneumoniae. However, certain sequences likely originated representing novel groups comparatively distant known phages.
Language: Английский
Citations
0
Data, Journal Year: 2024, Volume and Issue: 9(2), P. 22 - 22
Published: Jan. 26, 2024
The infections caused by various bacterial pathogens both in clinical and community settings represent a significant threat to public healthcare worldwide. growing resistance antimicrobial drugs acquired species causing healthcare-associated has already become life-threatening danger noticed the World Health Organization. Several groups or lineages of isolates, usually called ‘the clones high risk’, often drive spread within particular species. Thus, it is vitally important reveal track such mechanisms which they acquire antibiotic enhance their survival skills. Currently, analysis whole-genome sequences for isolates interest increasingly used these purposes, including epidemiological surveillance development prevention measures. However, availability uniformity data derived from genomic bottleneck investigations. With this dataset, we present results epidemiology 17,546 genomes dangerous pathogen, Acinetobacter baumannii. Important typing information, multilocus sequence (MLST)-based types (STs), intrinsic blaOXA-51-like gene variants, capsular (KL) oligosaccharide (OCL) types, CRISPR-Cas systems, cgMLST profiles are presented, as well assignment nine known international risk. presence genes also reported. These will be useful researchers field A. baumannii epidemiology, analysis, measure development.
Language: Английский
Citations
2
Carbohydrate Research, Journal Year: 2024, Volume and Issue: 544, P. 109249 - 109249
Published: Aug. 24, 2024
Language: Английский
Citations
2