Unraveling the interplay of the soil microbiome and (poly)phenol content in blueberry in response to disturbances

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 7, 2024

Aims Disturbances exert direct and indirect effects on plants through alterations of soil properties microbiota composition. This can induce stress, resulting in modifications plants’ phytochemical profile. turn affect the possibility for Indigenous people to engage cultural activities depending wild used as food or medicine. As a case study, we evaluated correlations between (poly)phenols Vaccinium angustifolium fruits, disturbances from mining hydroelectric activities, properties, microbiome Methods We collected fruit samples territories three communities eastern Canada. Fruits were analyzed their concentrations anthocyanins, proanthocyanidins other (poly)phenols. Soil microbial DNA was extracted reconstruct bacterial fungal communities. A secondary subset measure properties. Relationships soil, investigated using multivariate analyses. Results affected microbiome, but not (poly)phenol content. Two classes unaffected by disturbances, Bacilli Desulfitobacteriia, positively correlated with levels proanthocyanidines delphinidin-, cyanidin-, petunidin-3-glucoside fruits. Conclusion did content V. However, mine may contaminate fruits pollutants detrimental human health, which should be before drawing conclusions about effect plant nutritional medicinal Some seem enhance (poly)phenolic suggesting that strategy could developed enhancing this culturally salient species.

Language: Английский

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Microbiodiversity Landscape Present in the Mine-Tailings of the “Sierra de Huautla” Biosphere Reserve, Mexico

Water Air & Soil Pollution, Journal Year: 2024, Volume and Issue: 235(8)

Published: July 19, 2024

Abstract Large-scale mining activities generate significant amounts of waste that accumulates in the environment. These wastes, known as mine tailings, contain high levels heavy metals, posing risks to human health and causing severe damage ecosystems. In this study, we determined metal content tailings Sierra de Huautla Biosphere Reserve (REBIOSH), Mexico, investigated their effect on microbial composition. One sites historically contaminated with metals was sampled three different locations, labeled S1, S2, S3. A fourth site free (S4) also used a control. Our results showed As, Pb, Cd, Ag, potentially dangerous exceed thresholds set by international regulatory agencies. Metal contamination indices indicated moderate extreme enrichment for potential ecological risks. metagenomic study core specie-specific microbiome covered Sinimarinibacterium flocculans , Jiangella anatolica Thiobacillus denitrificans Fontimonas thermophile Sphingomonas koreensis . may be associated processing metals. comparative using ALDEx2 revealed less represented species like Variovorax paradoxus Usitatibacter rugosus palustris Sphingosinicella microcystinivorans Sphingobium yanoikuyae Stella humosa serve markers metal-contaminated environments. addition, detected rare or low-abundance belonging phylum Armatimonadota Candidatus Melainobacteriota Saccharimonadota Chlamydiota Deinococcota Elusimicrobiota Bacillota Rhodothermota Verrucomicrobiota which could play an important role ecosystems Also, found site-specific taxonomic representatives such Nitrososphaera gargensis Nitrospira nitrificans dominating S3 ecosystem; Ensifer aridi (S2 S1), N. (S2), while Reyranella soli dominate S1 soil. organisms crucial nitrogen access oligotrophic environments underscore adaptability life conditions. This is first comprehensive composition Mexican geography can provide future guidance management biological treatment wastes.

Language: Английский

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Production of birnessite-type manganese oxides by biofilms from oxygen-supplemented biological activated carbon (BAC) filters

Environmental Science Water Research & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The application of a novel enrichment approach revealed the potential manganese-oxidizing bacteria (MnOB) from full-scale biofilters treating wastewater to produce manganese oxides as crystalline, efficient birnessite catalysts.

Language: Английский

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Metagenome Assembled Genomes (MAG) Facilitate a Better Understanding of Microbially-mediated Heavy Metal Resistance in Soils from a Former Nuclear Materials Production Facility

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 23, 2023

Abstract Shotgun metagenomes is a repository of all the genes present in an environmental sample. With recent advancements bioinformatic techniques, it now possible to in-silico retrieve sequences that belong specific taxa, followed by assembly and annotation obtained are called as metagenome-assembled genome (MAG), which facilitates better understanding metabolic other traits without having culture microorganism. We applied MAG technique using nf-core/mag pipeline on shotgun metagenome from soil ecosystem has long-term co-contamination with radionuclides (mainly uranium), heavy metals (mercury, nickel etc.) organic compounds. Annotation MAGs was performed SPAdes MEGAHIT genomes were binned taxonomically classified GTDBTk CAT toolkits within nf-core/mag. Additional annotations done Prokka Prodigal dRep program used choose for further analysis. Initial analysis resulted total 254 met high-quality standard completeness > 95% contamination < 5%, accounting 26.67% (Fig SI-1). After bin refinement de-replication, 27 (18 Winter season 9 Summer season) reconstructed. These span across 6 bacterial phyla most predominant ones Proteobacteria, Bacteroidetes, Cyanobacteria regardless season. Overall, Arthrobacter found be one robust Over 1749 putatively involved crucial metabolism elements viz. nitrogen, phosphorous, sulfur 598 encoding enzymes resistance cadmium, zinc, chromium, arsenic copper. In summary, this project enhances our conferring uranium contaminated soils.

Language: Английский

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